DNA microarray analysis of the expression of the genes encoding the major enzymes in ethanol production during glucose and xylose co-fermentation by metabolically engineered Saccharomyces yeast

Enzyme and Microbial Technology

Volumn 33, Issue 1, 2003, Pages 19-28

  Sedlak, Miroslav ^a   Edenberg, Howard J ^b   Ho, Nancy W Y ^a  

^a PURDUE UNIVERSITY   (United States)

^b INDIANA UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

Glucose; Glycolytic pathway; Microarray; Production of ethanol; Saccharomyces cerevisiae; Xylose

Indexed keywords

BIOMASS; DNA; ENZYME KINETICS; ETHANOL; FERMENTATION; METABOLISM; YEAST;

CO-FERMENTATION; GENE ENCODING;

GENETIC ENGINEERING;

ALCOHOL; FUNGAL ENZYME; GLUCOSE; UNCLASSIFIED DRUG; XYLITOL DEHYDROGENASE; XYLOSE; XYLOSE REDUCTASE; XYLULOKINASE;

ALCOHOL PRODUCTION; ARTICLE; CHROMOSOME; DNA MICROARRAY; ENZYME ACTIVITY; ENZYME ANALYSIS; FERMENTATION; FUNGAL BIOMASS; FUNGAL GENETICS; FUNGAL STRAIN; GENE EXPRESSION PROFILING; GENETIC ENGINEERING; METABOLIC ENGINEERING; NONHUMAN; PLASMID; SACCHAROMYCES CEREVISIAE;

SACCHAROMYCES; SACCHAROMYCES CEREVISIAE;

EID: 0037735189     PISSN: 01410229     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0141-0229(03)00067-X     Document Type: Article

References (10)

1. Ho N.W.Y., Chen Z., Brainard A. Genetically engineered Saccharomyces yeast capable of effective cofermentation of glucose and xylose. Appl. Environ. Microbiol. 64:1998;1852-1859.
2. Ho NWY, Tsao GT. Recombinant Yeasts for Effective Fermentation of Glucose and Xylose U.S. Patent Application #08/148, 581, awarded August 1998, Patent no. 5,789,210.
3. Ho NWY, Chen Z, Brainard AP, Sedlak M. Successful design and development of genetically engineered Saccharomyces yeasts for effective cofermentation of glucose and xylose from cellulosic biomass to fuel ethanol. In: Tsao GT, editor. Advances in biochemical engineering/biotechnology. Berlin: Springer; 1999.
4. Ho NWY, Chen Z, Brainard AP, Sedlak M. Genetically engineered Saccharomyces yeasts for conversion of cellulosic biomass to environmentally friendly transportation fuel ethanol. In: ACS Symposium Series 767, American Chemical Society; 2000. p. 142-59.
5. Ho NWY, Chen ZD. Stable recombinant yeasts capable of effective fermentation of both glucose and xylose. PCT Patent No. WO97/42307.
6. Li R-J, Kim D-H, Castanotto D, Westaway S, Rossi JJ. RNA preparation from yeast cells. In: Krieg PA, editor. A laboratory guide to RNA: isolation analysis, and synthesis. Wiley-Liss, Inc.; 1996. p. 43-50.
7. Maitra P.K., Lobo Z. A kinetic study of glycolytic enzyme synthesis in yeast. J. Biol. Chem. 246:1971;475-488.
8. Bradford M.M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Chem. 72:1976;248-254.
9. Kotter P., Ciriacy M. Xylose fermentation by Saccharomyces cerevisiae. Appl. Microbiol. Biotechnol. 38:1993;776-783.
10. Walfridsson M., Anderlund M., Bao X., Hahn-Hagerdal B. Expression of different levels of enzymes from the Pichia stipitis XYL1 and XYL2 genes in Saccharomyces cerevisiae and its effects on product formation during xylose utilization. Appl. Microbiol. Biotechnol. 48:1997;218-224.