Light microscopy and electron microscopy of Nephroselmis spinosa sp. nov. (Prasinophyceae, Chlorophyta)

Journal of Phycology

Volumn 39, Issue 3, 2003, Pages 590-599

  Suda, Shoichiro ^a,b  

^a MARINE BIOTECHNOLOGY INSTITUTE   (Japan)

^b UNIVERSITY OF THE RYUKYUS   (Japan)

Author keywords

Morphology; Nephroselmis spinosa; Prasinophyceae; Scale morphology; Taxonomy; Ultrastructure

Indexed keywords

TAXONOMY; ULTRASTRUCTURE;

ALGAE; CHLOROPHYTA; EUKARYOTA; NEPHROSELMIS ASTIGMATICA; NEPHROSELMIS OLIVACEA; NEPHROSELMIS SPINOSA; PHASEOLUS (ANGIOSPERM); PRASINOPHYCEAE;

EID: 0037671550     PISSN: 00223646     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1529-8817.2003.01194.x     Document Type: Article

References (21)

1. Andersen, R. A., Barr, D. J. S., Lynn, D. H., Melkonian, M., Moestrup, Ø. & Sleigh, M. A. 1991. Terminology and nomenclature of the cytoskeletal elements associated with the flagellar/ciliary apparatus in protists. Protoplasma 164:1-8.
2. Butcher, R. W. 1959. An introductory account of the smaller algae of British Coastal Waters. Part I. Introduction and Chlorophyceae. Fish. Invest. 4:1-74.
3. Inouye, I. & Pienaar, R. N. 1984. Light and electron microscope observations on Nephroselmis astigmatica sp. nov. (Prasinophyceae). Nord. J. Bot. 4:409-23.
4. Manton, I., Rayns, D. G., Ettl, H. & Parke, M. 1965. Further observations on green flagellates with scaly flagella: the genus Heteromastix Korschikov. J. Mar. Biol. Assoc. UK 45:241-55.
5. Marin, B., Matzke, C. & Melkonian, M. 1993. Flagellar hairs of Tetraselmis (Prasinophyceae): ultrastructural types and intrageneric variation. Phycologia 32:213-22.
6. Marin, B. & Melkonian, M. 1994. Flagellar hairs in prasinophytes (Chlorophyta): ultrastructure and distribution on the flagellar surface. J. Phycol. 30:659-78.
7. Melkonian, M. 1989. Flagellar apparatus ultrastructure in Mesostigma viride (Prasinophyceae). Pl. Syst. Evol. 164:93-122.
8. Moestrup, Ø. 1983. Studies on Nephroselmis and its allies (Prasinophyceae). I. The question of the genus Bipedinomonas. Nord. J. Bot. 3:609-27.
9. Moestrup, Ø. & Ettl, H. 1979. A light and electron microscopical study of Nephroselmis olivacea (Prasinophyceae). Opera Bot. 49:1-39.
10. Moestrup, Ø. & Hori, T. 1989. Ultrastructure of the flagellar apparatus of in Pyramimonas octopus (Prasinophyceae). II. Flagellar roots, connecting fibers and numbering of individual flagella in green algae. Protoplasma 148:41-56.
11. Nakayama, T., Kawachi, M. & Inouye, I. 2000. Taxonomy and the phylogenetic position of a new prasinophycean alga, Crustmastix didyma gen. & sp. nov. (Chlorophyta). Phycologia 39:337-48.
12. Provasoli, L. 1968. Media and prospects for the cultivation of marine algae. In Watanabe, A. & Hattori, A. [Eds.] Culture and Collections of Algae. Proceedins of the U.S.-Japanese Conference, Hakone, 1966, Japanese Society of Plant Physiology, Tokyo, pp. 63-75.
13. Reynolds, E. S. 1963. The use of lead citrate at high pH as an electronopaque stain in electron microscopy. J. Cell Biol. 17:208-12.
14. Schiller, J. 1995. Die planktonischen Vegetationen des adriatischen Meers. B. Chrysomonadina, Heterokontae, Cryptomonadina, Eugleninae, Volvocales. I. Systematischer Teil. Arch. Protistenk. 63:59-193.
15. Spurr, A. R. 1969. A low-viscosity epoxy resin embedding medium for electron microscopy. J. Ultrastruct. Res. 96:31-43.
16. Suda, S., Watanabe, M. M. & Inouye, I. 1989. Evidence for sexual reproduction in the primitive green alga Nephroselmis olivacea (Prasinophyceae). J. Phycol. 25:596-600.
17. Sym, S. D. & Pienaar, R. N. 1993. The class Prasinophyceae. In Round, F. E. & Chapman, D. J. [Eds.] Progress in Phycological Research. Vol. 9. Biopress Ltd., Bristol, pp. 281-376.
18. Thomsen, H. A. 1986. A survey of the smallest eucaryotic organisms of the marine phytoplankton. Can. Bull. Fish. Aquat. Sci. 214:121-58.
19. Tseng, C. K., Chen, J., Zhang, Z. & Zhang, H. 1994. Light and electron microscope observations on Nephroselmis gaoae sp. nov. (Prasinophyceae). Chin. J. Oceanol. Limnol. 12:201-7.
20. Tsutsui, K., Kumon, H., Ichikawa, H. & Tawara, J. 1976. Preparative methods for suspended biological materials for SEM by using polycationic substance layer. J. Electro. Microsc. 25:163-8.
21. Watanabe, M. M. & Hiroki, M., Eds. 1997. NIES Collection. List of Strains, 5th ed. Microalgae and Protozoa. Microbial Culture Collection, National Institute for Environmental Studies, Tsukuba, 140 pp.