Substrate recognition and molecular mechanism of fatty acid hydroxylation by cytochrome P450 from Bacillus subtilis: Crystallographic, spectroscopic, and mutational studies

Journal of Biological Chemistry

Volumn 278, Issue 11, 2003, Pages 9761-9767

  Lee, Dong Sun ^a   Yamada, Akari ^a   Sugimoto, Hiroshi ^a   Matsunaga, Isamu ^b   Ogura, Hisashi ^b   Ichihara, Kosuke ^c   Adachi, Shin ichi ^a   Park, Sam Yong ^a,d   Shiro, Yoshitsugu ^a  

^a RIKEN HARIMA INSTITUTE   (Japan)

^b OSAKA CITY UNIVERSITY GRADUATE SCHOOL OF MEDICINE   (Japan)

^c Osaka Seikei College   (Japan)

^d YOKOHAMA CITY UNIVERSITY   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

CRYSTAL STRUCTURE; CRYSTALLOGRAPHY; HYDROPHOBICITY; HYDROXYLATION; SUBSTRATES;

MOLECULAR MECHANISM;

FATTY ACIDS;

ARGININE; CARBOXYLIC ACID; CHLORIDE PEROXIDASE; CYTOCHROME P450; FATTY ACID; GLUTAMIC ACID; GUANIDINE DERIVATIVE; HEME DERIVATIVE; HISTIDINE; HYDROGEN PEROXIDE; LONG CHAIN FATTY ACID; MYRISTIC ACID; CYSTEINE; LIGAND; OXYGEN; RECOMBINANT PROTEIN;

ARTICLE; BACILLUS SUBTILIS; CRYSTAL STRUCTURE; CRYSTALLOGRAPHY; ELECTRICITY; ENZYME ACTIVE SITE; ENZYME SUBSTRATE COMPLEX; HYDROPHOBICITY; HYDROXYLATION; MOLECULAR RECOGNITION; MOLECULAR WEIGHT; MUTATION; NONHUMAN; PRIORITY JOURNAL; SPECTROSCOPY; BINDING SITE; CHEMICAL MODEL; CHEMICAL STRUCTURE; CHEMISTRY; ENZYME SPECIFICITY; ENZYMOLOGY; ESCHERICHIA COLI; METABOLISM; NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY; PROTEIN BINDING; PROTEIN CONFORMATION; PROTEIN FOLDING; PROTEIN TERTIARY STRUCTURE; RAMAN SPECTROMETRY; SITE DIRECTED MUTAGENESIS; SPECTROPHOTOMETRY; X RAY CRYSTALLOGRAPHY;

BACILLUS SUBTILIS; POSIBACTERIA;

ARGININE; BACILLUS SUBTILIS; BINDING SITES; CRYSTALLOGRAPHY, X-RAY; CYSTEINE; CYTOCHROME P-450 ENZYME SYSTEM; ESCHERICHIA COLI; FATTY ACIDS; GLUTAMIC ACID; HISTIDINE; LIGANDS; MAGNETIC RESONANCE SPECTROSCOPY; MODELS, CHEMICAL; MODELS, MOLECULAR; MUTAGENESIS, SITE-DIRECTED; MYRISTIC ACID; OXYGEN; PROTEIN BINDING; PROTEIN CONFORMATION; PROTEIN FOLDING; PROTEIN STRUCTURE, TERTIARY; RECOMBINANT PROTEINS; SPECTROPHOTOMETRY; SPECTRUM ANALYSIS, RAMAN; SUBSTRATE SPECIFICITY;

EID: 0037646516     PISSN: 00219258     EISSN: None     Source Type: Journal    
DOI: 10.1074/jbc.M211575200     Document Type: Article

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