Affinity thermoprecipitation and recovery of biotinylated biomolecules via a mutant streptavidin-smart polymer conjugate

Bioconjugate Chemistry

Volumn 14, Issue 3, 2003, Pages 575-580

  Malmstadt, Noah ^a   Hyre, David E ^a   Ding, Zhongli ^a   Hoffman, Allan S ^a   Stayton, Patrick S ^a  

^a University of Washington   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ACRYLIC MONOMERS; COENZYMES; CONJUGATED POLYMERS; EFFICIENCY; HYDROGELS; MACROMOLECULES;

CO-PRECIPITATES; IMMUNOGLOBULIN G; POLY(N-ISOPROPYLACRYLAMIDE); POLYMER CONJUGATE; SMART POLYMERS; STREPTAVIDIN; TARGET MOLECULE; TEMPERATURE RESPONSIVE POLYMERS; THERMAL STIMULI; WILD TYPES;

OLIGONUCLEOTIDES;

BIOTIN; IMMUNOGLOBULIN G; OLIGONUCLEOTIDE; POLY(N ISOPROPYLACRYLAMIDE); STREPTAVIDIN;

ARTICLE; BINDING AFFINITY; BINDING KINETICS; BIOTINYLATION; CONJUGATION; INTERMETHOD COMPARISON; MACROMOLECULE; MOLECULAR DYNAMICS; MOLECULAR INTERACTION; MOLECULAR MECHANICS; PHASE TRANSITION; PRECIPITATION; SEPARATION TECHNIQUE; SITE DIRECTED MUTAGENESIS; THERMAL STIMULATION;

EID: 0037508878     PISSN: 10431802     EISSN: None     Source Type: Journal    
DOI: 10.1021/bc020055l     Document Type: Article

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