Enzyme histochemistry on human placental trophoblasts: The effect of fixation delay on enzyme activity

Histochemistry and Cell Biology

Volumn 113, Issue 4, 2000, Pages 287-292

  Matsubara, Shigeki ^a   Takizawa, Toshihiro ^a   Sato, Ikuo ^a  

^a JICHI MEDICAL UNIVERSITY   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

ACID PHOSPHATASE; ALKALINE PHOSPHATASE; CYTOCHROME C OXIDASE; GLUCOSE 6 PHOSPHATASE;

ARTICLE; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME HISTOCHEMISTRY; HUMAN; HUMAN CELL; PLACENTA; PRIORITY JOURNAL; TISSUE FIXATION; TROPHOBLAST;

EID: 0037488815     PISSN: 09486143     EISSN: None     Source Type: Journal    
DOI: 10.1007/s004180000134     Document Type: Article

References (22)

1. Alberts B, Bray B, Lewis J, Raff M, Roberts K, Watson JD (1994a) Vesicular traffic in the secretory and endocytic pathways. In: Alberts B, Bray B, Lewis J, Raff M, Roberts K, Watson JD (eds) Molecular biology of the cell, 3rd edn. Garland, New York, pp 599-651
2. Alberts B, Bray B, Lewis J, Raff M, Roberts K, Watson JD (1994b) Energy conversion: mitochondria and chloroplasts. In: Alberts B, Bray B, Lewis J, Raff M, Roberts K, Watson JD (eds) Molecular biology of the cell, 3rd edn. Garland, New York, pp 653-720
3. Asaka Y, Watanabe J, Kanai K, Kanamura S (1991) High glucose-6-phosphatase activity in non-pigmented epithelial cells of rabbit ciliary body. J Histochem Cytochem 39:1113-1120
4. Fujioka A (1993) Fixation. In: Ogawa K, Barka T (eds) Electron microscopic cytochemistry and immunocytochemistry in biomedicine. CRC Press, Boca Raton, pp 9-16
5. Hayat MA (1973) Specimen preparation. In: Hayat MA (ed) Electron microscopy of enzymes. Van Nostrand Reinhold, New York, pp 1-43
6. Hoof VO van, Deng JT, Broe ME de (1997) How do plasma membranes reach the circulation? Clin Chim Acta 266:23-31
7. Ito T, Ando T, Mayahara H, Miyajima H, Ogawa K (1991) Postmortem changes in the rat kidney. II. Histopathological, electron microscopical, and enzyme histochemical studies of postmortem changes at 0°C. Acta Histochem Cytochem 24:153-166
8. Jones CJ, Fox H (1980) An ultrastructural and ultrahistochemical study of the human placenta in maternal pre-eclampsia. Placenta 1:61-76
9. Kaufmann P (1985) Influence of ischemia and artificial perfusion on placental ultrastructure and morphometry. Contrib Gynecol Obstet 13:18-26
10. Matsubara S, Tamada T (1988) Presence of nerve fibers in the human umbilical cord. An electron microscopical histochemical proof. Acta Obstet Gynaecol Jpn 40:1775-1776
11. Matsubara S, Tamada T, Saito T (1987a) Ultracytochemical localizations of adenylate cyclase, guanylate cyclase and cyclic 3′,5′-nucleotide phosphodiesterase activity on the trophoblast in the human placenta. Histochemistry 87:505-509
12. Matsubara S, Tamada T, Saito T (1987b) Ultracytochemical localizations of alkaline phosphatase and acid phosphatase activities in the human term placenta. Acta Histochem Cytochem 20:283-294
13. Matsubara S, Sato I, Saito T (1997a) Ultrastructural localization and cytochemical characteristics of human placental ADP-degrading activity in normal and preeclamptic pregnancy. Trophoblast Res 9:121-129
14. Matsubara S, Minakami H, Sato I, Saito T (1997b) Decrease in cytochrome c oxidase activity detected cytochemically in the placental trophoblast of patients with pre-eclampsia. Placenta 18:255-259
15. Matsubara S, Yamada T, Minakami H, Sato I (1998a) Stimulated polymorphonuclear leukocytes in vaginal secretions from patients with preterm labor. Gynecol Obstet Invest 45:35-40
16. Matsubara S, Minakami H, Yamada T, Koike T, Izumi A, Takizawa T, Sato I (1998b) Stimulated Hofbauer cells in the placental villi from patients with second-trimester abortions. Acta Histochem Cytochem 31:447-452
17. Matsubara S, Takizawa T, Sato I (1999a) Glucose-6-phosphatase is present in normal and preeclamptic placental trophoblasts: ultrastructural enzyme-histochemical evidence. Placenta 20:81-85
18. Matsubara S, Yamada T, Minakami H, Watanabe T, Takizawa T, Sato I (1999b) Polymorphonuclear leukocytes in the fetal membranes are activated in patients with preterm delivery: ultrastructural and enzyme-histochemical evidence. Placenta 20: 185-188
19. Pavelka M, Pavelka R, Gerstner A (1979) Ultrastructure of the syncytiotrophoblast of the human placenta in EPH-gestosis. Gynecol Obstet Invest 10:177-185
20. Pearse AGE (1980) The chemistry and practice of fixation. In: Pearse AGE (ed) Histochemistry, theoretical and applied. Churchill Livingstone, Edinburgh, pp 97-158
21. Robinson JM, Karnovsky MJ (1983) Ultrastructural localization of some phosphatases with cerium. J Histochem Cytochem 31: 1197-1208
22. Seligman AM, Karnovsky MJ, Wasserkrug HL, Hanker JS (1968) Nondroplet ultrastructural demonstration of cytochrome oxidase activity with a polymerizing osmiophilic reagent, diaminobenzidine (DAB). J Cell Biol 38:1-14