The wbiA locus is required for the 2-O-acetylation of lipopolysaccharides expressed by Burkholderia pseudomallei and Burkholderia thailandensis

FEMS Microbiology Letters

Volumn 218, Issue 2, 2003, Pages 323-328

  Brett, Paul J ^a   Burtnick, Mary N ^a   Woods, Donald E ^a  

^a Health Sciences Centre Calgary   (Canada)

Author keywords

Burkholderia species; O antigen; trans Acylase; Virulence determinant

Indexed keywords

LIPOPOLYSACCHARIDE;

ACETYLATION; ANTIGEN EXPRESSION; ARTICLE; BURKHOLDERIA; BURKHOLDERIA PSEUDOMALLEI; BURKHOLDERIA THAILANDENSIS; CONTROLLED STUDY; GENE ACTIVITY; MOLECULE; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL;

ACETYLATION; AMINO ACID SEQUENCE; BURKHOLDERIA; BURKHOLDERIA PSEUDOMALLEI; DNA, BACTERIAL; EPITOPES; GENES, BACTERIAL; HUMANS; LIPOPOLYSACCHARIDES; LOCUS CONTROL REGION; MAGNETIC RESONANCE SPECTROSCOPY; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; O ANTIGENS; SEQUENCE ALIGNMENT;

BURKHOLDERIA; BURKHOLDERIA PSEUDOMALLEI; BURKHOLDERIA THAILANDENSIS;

EID: 0037469348     PISSN: 03781097     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0378-1097(02)01176-X     Document Type: Article

References (23)

1. Dance, D.A.B. (1996) in: Melioidosis (Cook, G.C.E., Ed.), pp. 925-930. W.B. Saunders, London.
2. Woods D.E., DeShazer D., Moore R.A., Brett P.J., Burtnick M.N., Reckseidler S.L., Senkiw M.D. Current studies on the pathogenesis of melioidosis. Microbes Infect. 1:1999;157-162.
3. Charuchaimontri C., Suputtamongkol Y., Nilakul C., Chaowagul W., Chetchotisakd P., Lertpatanasuwun N., Intaranongpai S., Brett P.J., Woods D.E. Antilipopolysaccharide II: an antibody protective against fatal melioidosis. Clin. Infect. Dis. 29:1999;813-818.
4. Bryan L., Wong S., Woods D., Dance D., Chaoagul W. Passive protection of diabetic rats with antisera specific for the polysaccharide portion of lipopolysaccharide from Pseudomonas pseudomallei. Can. J. Infect. Dis. 5:1994;170-178.
5. DeShazer D., Brett P.J., Woods D.E. The type II O-antigenic polysaccharide moiety of Burkholderia pseudomallei lipopolysaccharide is required for serum resistance and virulence. Mol. Microbiol. 30:1998;1081-1100.
6. Ho M., Schollaardt T., Smith M.D., Perry M.B., Brett P.J., Chaowagul W., Bryan L.E. Specificity and functional activity of anti-Burkholderia pseudomallei polysaccharide antibodies. Infect. Immun. 65:1997;3648-3653.
7. Brett P.J., Woods D.E. Structural and immunological characterization of Burkholderia pseudomallei O-polysaccharide-flagellin protein conjugates. Infect. Immun. 64:1996;2824-2828.
8. Knirel Y.A., Paramonov N.A., Shashkov A.S., Kochetkov N.K., Yarullin R.G., Farber S.M., Efremenko V.I. Structure of the polysaccharide chains of Pseudomonas pseudomallei lipopolysaccharides. Carbohydr. Res. 233:1992;185-193.
9. Perry M.B., MacLean L.L., Schollaardt T., Bryan L.E., Ho M. Structural characterization of the lipopolysaccharide O antigens of Burkholderia pseudomallei. Infect. Immun. 63:1995;3348-3352.
10. Brett P.J., DeShazer D., Woods D.E. Burkholderia thailandensis sp. nov., a Burkholderia pseudomallei-like species. Int. J. Syst. Bacteriol. 48:1998;317-320.
11. Burtnick M.N., Brett P.J., Woods D.E. Molecular and physical characterization of Burkholderia mallei O-antigens. J. Bacteriol. 184:2002;849-852.
12. Sambrook, J., Fritsch, E. and Maniatis, T. (1989) Molecular Cloning: A Laboratory Manual, 2nd edn. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
13. Altschul S.F., Madden T.L., Schaffer A.A., Zhang J., Zhang Z., Miller W., Lipman D.J. Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res. 25:1997;3389-3402.
14. Moore R.A., DeShazer D., Reckseidler S., Weissman A., Woods D.E. Efflux-mediated aminoglycoside and macrolide resistance in Burkholderia pseudomallei. Antimicrob. Agents Chemother. 43:1999;465-470.
15. DeShazer D., Brett P.J., Carlyon R., Woods D.E. Mutagenesis of Burkholderia pseudomallei with Tn5-OT182: isolation of motility mutants and molecular characterization of the flagellin structural gene. J. Bacteriol. 179:1997;2116-2125.
16. Hitchcock P.J., Brown T.M. Morphological heterogeneity among Salmonella lipopolysaccharide chemotypes in silver-stained polyacrylamide gels. J. Bacteriol. 154:1983;269-277.
17. Towbin H., Staehelin T., Gordon J. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc. Natl. Acad. Sci. USA. 76:1979;4350-4354.
18. Tsai C.M., Frasch C.E. A sensitive silver stain for detecting lipopolysaccharides in polyacrylamide gels. Anal. Biochem. 119:1982;115-119.
19. Dubois M., Gilles K.A., Hamilton J.K., Rebers P.A., Smith F. Colorimetric method for determination of sugars and related substances. Anal. Chem. 28:1956;350-356.
20. Slauch J.M., Lee A.A., Mahan M.J., Mekalanos J.J. Molecular characterization of the oafA locus responsible for acetylation of Salmonella typhimurium O-antigen: OafA is a member of a family of integral membrane trans-acylases. J. Bacteriol. 178:1996;5904-5909.
21. Simon R., Priefer U., Puhler A. A broad host range mobilization system for in vivo genetic eningeering: transposon mutagenesis in Gram-negative bacteria. Bio/Technology. 1:1983;784-791.
22. Burtnick M., Bolton A., Brett P., Watanabe D., Woods D. Identification of the acid phosphatase (acpA) gene homologues in pathogenic and non-pathogenic Burkholderia spp. facilitates TnphoA mutagenesis. Microbiology. 147:2001;111-120.
23. Schweizer, H., Klassen, T. and Hoang, T. (1996) Improved methods for gene analysis and expression in Pseudomonas spp. In: Molecular Biology of Pseudomonads (Nakazawa, T., Furukawa, K., Haas, D. and Silver, S., Eds.). American Society for Microbiology, Washington, DC.