The regulatory elements of the Mycobacterium tuberculosis gene Rv3881c function efficiently in Escherichia coli

FEMS Microbiology Letters

Volumn 218, Issue 2, 2003, Pages 365-370

  Satchidanandam, Vijaya ^a   Amara, Rama Rao ^a,b   Uchil, Pradeep Devappa ^a   Singh, Varsha ^a  

^a INDIAN INSTITUTE OF SCIENCE   (India)

^b EMORY UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

Mycobacterium tuberculosis; Promoter; Ribosome binding site; Rv3881c

Indexed keywords

ARTICLE; BACTERIAL GENE; BINDING SITE; ESCHERICHIA COLI; GENE EXPRESSION; GENE FUNCTION; GENETIC TRANSCRIPTION; MYCOBACTERIUM BOVIS; MYCOBACTERIUM KANSASII; MYCOBACTERIUM SZULGAI; MYCOBACTERIUM TUBERCULOSIS; NONHUMAN; OPEN READING FRAME; PRIORITY JOURNAL; PROMOTER REGION;

CORYNEBACTERINEAE; ESCHERICHIA COLI; MYCOBACTERIUM; MYCOBACTERIUM BOVIS; MYCOBACTERIUM BOVIS BCG; MYCOBACTERIUM KANSASII; MYCOBACTERIUM SZULGAI; MYCOBACTERIUM TUBERCULOSIS;

EID: 0037469274     PISSN: 03781097     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0378-1097(02)01185-0     Document Type: Article

References (17)

1. Ohara N., Yamada T. Recombinant BCG vaccines. Vaccine. 19:2001;4089-4098.
2. Horwitz M.A., Harth G., Dillon B.J., Maslesa-Galic' S. Recombinant Bacillus Calmette-Guerin (BCG) vaccines expressing the Mycobacterium tuberculosis 30-kDa major secretory protein induce greater protective immunity against tuberculosis than conventional BCG vaccines in a highly susceptible animal model. Proc. Natl. Acad. Sci. USA. 97:2000;13853-13858.
3. Stover C.K., de la Cruz V.F., Fuerst T.R., Burlein J.E., Benson L.A., Bennett L.T., Bansal G.P., Young J.F., Lee M.H., Hatfull G.F. New use of BCG for recombinant vaccines. Nature. 351:1991;456-460.
4. DasGupta S.K., Bashyam M.D., Tyagi A.K. Cloning and assessment of mycobacterial promoters by using a plasmid shuttle vector. J. Bacteriol. 175:1993;5186-5192.
5. Mehra V., Sweetser D., Young R. Efficient mapping of protein antigenic determinants. Proc. Natl. Acad. Sci. USA. 83:1986;7013-7017.
6. Shinnick T.M. The 65-kilodalton antigen of Mycobacterium tuberculosis. J. Bacteriol. 169:1987;1080-1088.
7. Thole J.E., Dauwerse H.G., Das P.K., Groothuis D.G., Schouls L.M., van Embden J.D. Cloning of Mycobacterium bovis BCG DNA and expression of antigens in Escherichia coli. Infect. Immun. 50:1985;800-806.
8. Amara R.R., Satchidanandam V. Analysis of a genomic DNA expression library of Mycobacterium tuberculosis using tuberculosis patient sera: evidence for modulation of host immune response. Infect. Immun. 64:1996;3765-3771.
9. Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C., Harris D., et al. Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence. Nature. 393:1998;537-544.
10. Amara R.R., Satchidanandam V. Differential immunogenicity of novel Mycobacterium tuberculosis antigens derived from live and dead bacilli. Infect. Immun. 65:1997;4880-4882.
11. DasGupta S.K., Jain S., Kaushal D., Tyagi A.K. Expression systems for study of mycobacterial gene regulation and development of recombinant BCG vaccines. Biochem. Biophys. Res. Commun. 246:1998;797-804.
12. Prasher D.C., Eckenrode V.K., Ward W.W., Prendergast F.G., Cormier M.J. Primary structure of the Aequorea victoria green-fluorescent protein. Gene. 111:1992;229-233.
13. Amara R.R., Shanti S., Satchidanandam V. Characterization of novel immunodominant antigens of Mycobacterium tuberculosis. Microbiology. 144:1998;1197-1203.
14. Gonzalez-y-Merchand J.A., Colston M.J., Cox R.A. The rRNA operons of Mycobacterium smegmatis and Mycobacterium tuberculosis: comparison of promoter elements and of neighbouring upstream genes. Microbiology. 142:1996;667-674.
15. Sambrook, J., Fritsch, E.F. and Maniatis, T. (1989) Molecular Cloning: A Laboratory Manual, 2nd edn. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
16. Bashyam M.D., Kaushal D., Dasgupta S.K., Tyagi A.K. A study of mycobacterial transcriptional apparatus: identification of novel features in promoter elements. J. Bacteriol. 178:1996;4847-4853.
17. Bashyam M.D., Tyagi A.K. Identification and analysis of 'extended -10′ promoters from mycobacteria. J. Bacteriol. 180:1998;2568-2573.