Evaluation of pefabloc as a serine protease inhibitor during human-islet isolation

Transplantation

Volumn 75, Issue 4, 2003, Pages 462-466

  Rose, Natisha L ^a   Palcic, Monica M ^a   Helms, Lisa M H ^a   Lakey, Jonathan R T ^a  

^a UNIVERSITY OF ALBERTA   (Canada)

Author keywords

[No Author keywords available]

Indexed keywords

4 (2 AMINOETHYL)BENZENESULFONYL FLUORIDE; CHYMOTRYPSIN; ELASTASE; PROTEINASE; SERINE PROTEINASE INHIBITOR; TRYPSIN;

ARTICLE; CADAVER DONOR; CELL ISOLATION; CLINICAL ARTICLE; CONTROLLED STUDY; CRYOPRESERVATION; DRUG EFFECT; ENZYME ACTIVATION; ENZYME ACTIVITY; ENZYME INHIBITION; EVALUATION; HUMAN; HUMAN TISSUE; PANCREAS ISLET TRANSPLANTATION; PRIORITY JOURNAL; TIME SERIES ANALYSIS; TISSUE PRESERVATION;

ADULT; CELL SEPARATION; EVALUATION STUDIES; HUMANS; ISLETS OF LANGERHANS; ISLETS OF LANGERHANS TRANSPLANTATION; MIDDLE AGED; SERINE ENDOPEPTIDASES; SERINE PROTEINASE INHIBITORS; SULFONES;

EID: 0037468450     PISSN: 00411337     EISSN: None     Source Type: Journal    
DOI: 10.1097/01.TP.0000046537.47139.CE     Document Type: Article

References (19)

1. Shapiro AM, Lakey JR, Ryan EA, et al. Islet transplantation in seven patients with type 1 diabetes mellitus using a glucocorticoid-free immunosuppressive regimen. N Engl J Med 2000; 343: 230.
2. Heiser A, Ulrichs K, Muller-Ruchholtz W. Prophylactic trypsin inhibition during the islet isolation procedure guarantees reproducible high porcine islet yields. Xenotransplantation 1994; 1: 66.
3. Lakey JR, Helms LM, Kin T, et al. Serine protease inhibition during islet isolation increases islet yield from human pancreases with prolonged ischemia. Transplantation 2001; 72: 565.
4. White SA, Djaballah H, Hughes DP, et al. A preliminary study of the activation of endogenous pancreatic exocrine enzymes during automated porcine islet isolation. Cell Transplant 1999; 8: 265.
5. Traverso LW, Abou-ZamZam AM. Activation of pancreatic proteolytic enzymes by commercial collagenases. Transplantation 1978; 25: 226.
6. Heiser A, Ulrichs K, Muller-Ruchholtz W. Isolation of porcine pancreatic islets: low trypsin activity during the isolation procedure guarantees reproducible high islet yield. J Clin Lab Anal 1994; 8: 407.
7. Niederau C, Fronhoffs K, Klonowski H, et al. Active pancreatic digestive enzymes show striking differences in their potential to damage isolated rat pancreatic acinar cells. J Lab Clin Med 1995; 125: 265.
8. Basir I, van der Burg MP, Scheringer M, et al. Improved outcome of pig islet isolation by Pefabloc inhibition of trypsin. Transplant Proc 1997; 29: 1939.
9. Cavanagh TJ, Fetterhoff TJ, Lonergan SC, et al. Collagenase selection. In: Lanza RP, Chick WL, eds. Pancreatic islet transplantation volume I: Procurement of pancreatic islets. Austin, TX, R. G. Landes 1994, p. 39-51.
10. Kneteman NM, Lakey JR, Warnock GL, et al. Human islet isolation after prolonged cold storage. Diabetes Nutr Metab 1992; 5S: 33.
11. Lakey JR, Warnock GL, Shapiro AM, et al. Intraductal collagenase delivery into the human pancreas using syringe loading or controlled perfusion. Cell Transplantation 1999; 8: 285.
12. London NJ, Chadwick D, Johnson PR, et al. Approaches to islet purification. In: Lanza RP, Chick WL, eds. Pancreatic islet transplantation volume I: Procurement of pancreatic islets. Austin, TX. R. G. Landes 1994, p. 25.
13. Ricordi C, Lacy PE, Finke EH, et al. Automated method for isolation of human pancreatic islets. Diabetes 1988; 37: 413.
14. Svendsen B, Blombäck B, Blombäck M, et al. Substrates for determination of trypsin, thrombin and thrombin-like enzymes. Folia Hematol Leipzig 1972; 98S: 446.
15. Del Mar EG, Largman C, Brodrick JW, et al. A sensitive new substrate for chymotrypsin. Anal Biochem 1979; 99: 316.
16. Del Mar EG, Largman C, Brodrick JW, et al. Substrate specificity of human pancreatic elastase 2. Biochemistry 1980; 19: 468.
17. Lakey JR, Warnock GL, Rajotte RV, et al. Variables in organ donors that affect the recovery of human islets of Langerhans. Transplantation 1996; 61: 1047.
18. Wolters GH, van Suylichem PT, van Deijnen JH, et al. Factors influencing the isolation process of islets of Langerhans. Horm Metab Res 1990; 25: 20.
19. Muhs BE, Patel S, Yee H, et al. Increased matrix metalloproteinase expression and activation following experimental acute pancreaitis. J Surg Res 2001; 101: 21.