Thermodynamic coupling of the loop and stem in unusually stable DNA hairpins closed by CG base pairs

Journal of the American Chemical Society

Volumn 125, Issue 8, 2003, Pages 2032-2033

  Moody, Ellen M ^a   Bevilacqua, Philip C ^a  

^a PENNSYLVANIA STATE UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

DNA;

ARTICLE; BASE PAIRING; CHEMICAL BOND; CHEMICAL STRUCTURE; MOLECULAR DYNAMICS; MOLECULAR INTERACTION; THERMODYNAMICS;

BASE PAIRING; CYTOSINE; DNA; GUANINE; NUCLEIC ACID CONFORMATION; THERMODYNAMICS;

EID: 0037467110     PISSN: 00027863     EISSN: None     Source Type: Journal    
DOI: 10.1021/ja029831q     Document Type: Article

References (23)

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11. Lowercase denotes nucleotides in the stem, and uppercase denotes the loop; N = A, C, G, or T; and B = C, G, or T. All oligonucleotides have the same three beginning (gga) and ending (tcc) nucleotides, so, in general, only loop and closing base pair nucleotides are provided.
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14. M values for CG to GC or AT closing base pair changes (Denkenberger, T. M.; Bevilacqua, P. C., unpublished data).
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17. 2EDTA], pH 7 for 3-4 h using a molecular weight cutoff of 1000. Oligonucleotides containing DAP were synthesized by the Nucleic Acids Facility on the Pennsylvania State University campus using reagents purchased from Glen Research.
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20. M < ±1.5 °C.
21. 37° for a CG to GC change of +3.06 kcal/mol.
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