Overexpression of a serine alkaline protease gene in Bacillus licheniformis and its impact on the metabolic reaction network

Enzyme and Microbial Technology

Volumn 32, Issue 6, 2003, Pages 706-720

  Çalik, Pinar ^a   Tomlin, Gregory C ^b   Oliver, Stephen G ^b   Özdamar, Tunçer H ^c  

^a MIDDLE EAST TECHNICAL UNIVERSITY   (Turkey)

^b UNIVERSITY OF MANCHESTER   (United Kingdom)

^c ANKARA UNIVERSITY   (Turkey)

Author keywords

Bacillus licheniformis; Metabolic flux analysis; Recombinant; Serine alkaline protease; SubC gene

Indexed keywords

BIOREACTORS; GENES; GLUCOSE; GROWTH KINETICS; MATHEMATICAL MODELS; METABOLISM;

METABOLIC FLUX DISTRIBUTIONS;

CLONING;

AMINO ACID; CARBOXYLIC ACID; GLUCOSE; PENTOSE PHOSPHATE; PROTEINASE; SERINE ALKALINE PROTEASE; TRICARBOXYLIC ACID; UNCLASSIFIED DRUG;

ARTICLE; BACILLUS LICHENIFORMIS; BACTERIAL GENE; BACTERIAL GENETICS; BACTERIAL METABOLISM; BIOPROCESS; BIOREACTOR; CONTROLLED STUDY; DATA ANALYSIS; DATA BASE; ENZYME ENGINEERING; ENZYME SYNTHESIS; GENE EXPRESSION; GENETIC CODE; GLYCOLYSIS; INTRACELLULAR TRANSPORT; MATHEMATICAL COMPUTING; MICROBIAL KINETICS; MOLECULAR CLONING; NONHUMAN; QUANTITATIVE GENETICS; REACTOR OPERATION; RECOMBINANT GENE; THEORETICAL MODEL; WILD TYPE;

BACILLUS LICHENIFORMIS;

EID: 0037457696     PISSN: 01410229     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0141-0229(03)00030-9     Document Type: Article

References (34)

1. Çalik P., Çalik G., Özdamar T.H. Oxygen transfer effects in serine alkaline protease fermentation by Bacillus licheniformis: use of citric acid as the carbon source. Enzyme Microb. Technol. 23:1998;451-461.
2. Çalik P., Çalik G., Takaç S., Özdamar T.H. Metabolic flux analysis for serine alkaline protease fermentation by Bacillus licheniformis in a defined medium: effects of the oxygen transfer rate. Biotechnol. Bioeng. 64:1999;151-167.
3. Çalik P., Çalik G., Özdamar T.H. Oxygen transfer strategy and its regulation effects in serine alkaline protease production by Bacillus licheniformis. Biotechnol. Bioeng. 69:2000;301-311.
4. Çalik P., Çalik G., Takaç S., Özdamar T.H. Metabolic flux analysis for serine alkaline protease production. Enzyme Microb. Technol. 27(10):2000;793-805.
5. Çalik P., Takaç S., Çalik G., Özdamar T.H. Serine alkaline protease overproduction capacity of Bacillus licheniformis. Enzyme Microb. Technol. 64:2000;45-60.
6. Çalik P., Özdamar T.H. Effects of carbon sources on metabolic capacity of Bacillus licheniformis for serine alkaline protease, neutral protease and α-amylase production. Biochem. Eng. J. 8(1):2001;61-81.
7. Maniatis T, Fritsch EF, Sambrook J. Molecular cloning - a laboratory manual. USA: Cold Spring Harbor Laboratory; 1982.
8. Bullock W.O., Fernandez J.M., Short J.M. XL1-Blue - a high-efficiency plasmid transforming recA Escherichia coli strain with beta-galactosidase selection. Biotechniques. 5:1987;376.
9. Yanisch-Perron C., Viera J., Messing J. Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors. Gene. 33:1985;103-119.
10. Çalik P. Bioprocess development for serine alkaline protease production, Ph.D. thesis, Ankara University, Ankara, 1998.
11. Posprech A., Neumann B. A versatile quick-prep of genomic DNA from Gram-positive bacteria. Trends Genet. 11(6):1995;217-218.
12. Vehmaanperä J. Transformation of Bacillus amyloliquefaciens by electroporation. FEMS Microbiol. Lett. 61:1989;165-170.
13. Jacobs M. Expression of the subtilisin Carlsberg-encoding gene in Bacillus licheniformis and Bacillus subtilis. Gene. 152:1995;69-74.
14. Sikorski R.S., Hieter P. A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae. Genetics. 122:1989;19-27.
15. Janniere L., Braund C., Ehrlich D. Structurally stable Bacillus subtilis cloning vectors. Gene. 87:1990;53-61.
16. Miller G.L. Use of dinitrosalicylic acid reagent for determination of reducing sugar. Anal. Chem. 31:1959;426-428.
17. Bidlinmeyer B.A., Cohen S.A., Tarvin T.L. Rapid analysis of amino acids using pre-column derivatization. J. Chromatogr. 336:1984;93-104.
18. Çalik P., Özdamar T.H. Mass flux balance based model and metabolic pathway engineering analysis for serine alkaline synthesis by Bacillus licheniformis. Enzyme Microb. Technol. 24:1999;621-635.
19. Çalik P., Özdamar T.H. Metabolic flux analysis for industrial microorganisms: a review. Rev. Chem. Eng. 18(6):2002;553-596.
20. Lopez J.M., Marks C.L., Freese E. The decrease of guanine nucleotides initiates sporulation of Bacillus subtilis. Biochim. Biophys. Acta. 587(2):1979;238-252.
21. Perego M., Spiegelman G.B., Hoch J.A. Structure of the gene for the transition state regulator, abrB: regulator synthesis is controlled by the spo0A sporulation gene in Bacillus subtilis. Mol. Microbiol. 2(6):1988;689-699.
22. Ferrari E., Henner D.J., Perego M., Hoch G.B. Transcription of Bacillus subtilis subtilisin and expression of subtilisin in sporulation mutants. J. Bacteriol. 170(1):1988;289-295.
23. Vasantha N., Freese E. Enzyme changes during Bacillus subtilis sporulation caused by deprivation of guanine nucleotides. J. Bacteriol. 144(3):1980;1119-1125.
24. Msadek T., Kunst F., Henner D., Klier A., Rapoport G., Dedonder R. Signal transduction pathway controlling synthesis of a class of degradative enzymes in Bacillus subtilis: expression of the regulatory genes and analysis of mutations in degS and degU. J. Bacteriol. 172:1990;824-834.
25. Bierbaum G., Giesecke U.E., Wandrey C. Analysis of nucleotide pools during protease production by Bacillus licheniformis. Appl. Microbiol. Biotechnol. 35:1991;725-730.
26. Ferrari E, Jarnagin AS, Schmidt BF. Commercial production of extracellular enzymes. In: Sonenshein AL, Hoch JA, Losick R, editors. Bacillus subtilis and other Gram-positive bacteria: biochemistry, physiology, and molecular genetics. Washington, DC: American Society for Microbiology; 1993. p. 917-37.
27. Wang L.F., Doi R.H. Complex character of senS, a novel gene regulating expression of extracellular-protein genes of Bacillus subtilis. J. Bacteriol. 172(4):1991;1939-1947.
28. Gaur N.K., Oppenheim J., Smith I. The Bacillus subtilis sin gene, a regulator of alternate developmental processes, codes for a DNA-binding protein. J. Bacteriol. 173:1991;678-686.
29. Perego M., Hoch G.B., Hoch J.A. Sequence analysis and regulation of the hpr locus, a regulatory gene for protease production and sporulation in Bacillus subtilis. J. Bacteriol. 170(6):1988;2560-2567.
30. Honjo M., Nakayama A., Fukazawa K., Kawamura K., Ando K., Hori M.et al. A novel Bacillus subtilis gene involved in negative control of sporulation and degradative-enzyme production. J. Bacteriol. 172(4):1990;1783-1790.
31. Priest F.G. Extracellular enzyme synthesis in the genus Bacillus. Bacteriol. Rev. 41:1977;71-753.
32. Frankena J., van Verseveld H.W., Stouthamer A.H. A continuous culture study of the bioenergetic aspects of growth and production of exocellular protease in Bacillus licheniformis. Appl. Microbiol. Biotechnol. 22:1985;169-176.
33. Frankena J., Koningstein G.M., Verseveld H.W., Stouthamer A.H. Effect of different limitations in chemostat cultures on growth and production of exocellular protease by Bacillus licheniformis. Appl. Microbiol. Biotechnol. 24:1986;106-112.
34. Çalik P., Çalik G., Özdamar T.H. Bioprocess development for serine alkaline protease production: a review. Rev. Chem. Eng. 17(Suppl. S):2001;1-62.