Inert site in a protein zinc cluster: Isotope exchange by high resolution mass spectrometry

Journal of the American Chemical Society

Volumn 125, Issue 11, 2003, Pages 3226-3227

  Blindauer, Claudia A ^a   Polfer, Nick C ^a   Keiper, Stella E ^a   Harrison, Mark D ^b   Robinson, Nigel J ^b   Langridge Smith, Pat R R ^a   Sadler, Peter J ^a  

^a UNIVERSITY OF EDINBURGH   (United Kingdom)

^b NEWCASTLE UNIVERSITY   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

METALLOTHIONEIN; PROTEIN ZINC COMPLEX; UNCLASSIFIED DRUG; ZINC COMPLEX;

ALPHA HELIX; ARTICLE; BINDING SITE; CHEMICAL REACTION; CYCLOTRON; FOURIER TRANSFORMATION; ION EXCHANGE; MASS SPECTROMETRY; METAL BINDING; MOLECULAR STABILITY; OXIDATION; REDUCTION;

CYCLOTRONS; FOURIER ANALYSIS; MASS SPECTROMETRY; MODELS, MOLECULAR; ZINC;

EID: 0037454329     PISSN: 00027863     EISSN: None     Source Type: Journal    
DOI: 10.1021/ja0284409     Document Type: Article

References (32)

1. See, for example: Metal Sites in Proteins and Models; Hill, H. A. O., Sadler, P. J., Thomson, A. J., Eds.; Springer-Verlag: Berlin, Heidelberg, 1999.
2. Babini, E.; Borsari, M.; Capozzi, F.; Eltis, L. D.; Luchinat, C. J. Biol. Inorg. Chem. 1999, 4, 692-700.
3. Gray, H. B.; Malmström, B. G.; Williams, R. J. P. J. Biol. Inorg. Chem. 2000, 5, 551-559.
4. (a) O'Halloran, T. V.; Culotta, V. C. J. Biol. Chem. 2000, 275, 25057-25060.
5. (b) Cobine, P. A.; George, G. N.; Jones, C. E.; Wickramasinghe, W. A.; Solioz, M.; Dameron, C. T. Biochemistry 2002, 41, 5822-5829.
6. (c) Drum, D. E.; Li, T.-K.; Vallee, B. L. Biochemistry 1969, 8, 3792-3797.
7. Palmiter, R. D. Proc. Natl. Acad. Sci. U.S.A. 1998, 95, 8428-8430.
8. Romero-Isart, N.; Vašák, M. J. Inorg. Biochem. 2002, 88, 388-396.
9. (a) Maret, W.; Larsen, K. S.; Vallee, B. L. Proc. Natl. Acad. Sci. U.S.A. 1997, 94, 2233-2237.
10. (b) Ejnik, J.; Munoz, A.; Gan, T.; Shaw, C. F., III; Petering, D. H. J. Biol. Inorg. Chem. 1999, 4, 784-790.
11. (c) Otvos, J. D.; Liu, X.; Li, H.; Shen, G.; Basti, M. In Metallothionein III; Suzuki, K. T., Imura, N., Kimura, M., Eds.; Birkhäuser Verlag; Basel, Boston, Berlin, 1993; pp 57-109.
12. Blindauer, C. A.; Harrison, M. D.; Robinson, A. K.; Parkinson, J. A.; Bowness, P. W.; Sadler, P. J.; Robinson, N. J. Mol. Microbiol. 2002, 45, 1421-1432.
13. Blindauer, C. A.; Harrison, M. D.: Parkinson, J. A.; Robinson, A. K.; Cavet, J. S.; Robinson, N. J.; Sadler, P. J. Proc. Natl. Acad. Sci. U.S.A. 2001, 98, 9593-9598.
14. Recombinant SmtA contains 55 amino acids (lacks N-terminal Met). Numbering scheme based on sequence deposited in Swissprot (www. expasy.ch). Similar zinc clusters are present in other cyanobacteria and pseudomonads (ref 8), and the key features of the inert Zn site are conserved in GatA from E. coli.
15. (a) Gehrig, P. M.; You, C.; Dallinger, R.; Gruber, C.; Brouwer, M.; Kägi, J. H. R.; Hunziker, P. E. Protein Sci. 2000, 9, 395-402.
16. (b) Hathout, Y.; Fabris, D.; Fenselau, C. Int. J. Mass Spectrom. 2001, 204, 1-6.
17. (c) Jensen, L. T.; Peltier, J. M.; Winge, D. R. J. Biol. Inorg. Chem. 1998, 3, 627-631.
18. (d) Merrifield, M. E.; Huang, Z.; Kille, P.; Stillman, M. J. J. Inorg. Biochem. 2002, 88, 153-172.
19. Amster, I. J. J. Mass Spectrom. 1996, 31, 1325-1337.
20. (a) Johnson, K. A.; Verhagen, M. F. J. M.; Brereton, P. S.; Adams, M. W. W.; Amster, I. J. Anal. Chem. 2000, 72, 1410-1418.
21. (b) He, F.; Hendrickson, C. L.; Marshall, A. G. J. Am. Soc. Mass Spectrom. 2000, 11, 120-126.
22. (c) Taylor, P. K.; Parks, B. Y. A.; Kurtz, D. M.; Amster, I. J. J. Biol. Inorg. Chem. 2001, 6, 201-206.
23. 70Zn 0.6%.
24. 70Zn 0.05% supplied by Oak Ridge National Laboratory, TN, as ZnO and dissolved in HCl).
25. 4Ac as eluant. The eluate (3.5 mL) was concentrated to ca. 23 μM in protein using an Amicon YM3 filter before FT-ICR-MS analysis. The final sample solutions (10 μM) also contained ubiquitin (5 μM), 30% MeOH, and 0.05% formic acid.
26. Krizek, B. A.; Merkle, D. L.; Berg, J. M. Inorg. Chem. 1993, 32, 937-940.
27. Kägi, J. H. R. In Metallothionein III; Suzuki, K. T., Imura, N., Kimura, M., Eds.; Birkhäuser Verlag: Basel, Boston, Berlin, 1993; pp 29-56.
28. Nettesheim, D. G.; Engeseth, H. R.; Otvos, J. D. Biochemistry 1985, 24, 6744-6751.
29. 2), was incubated with EDTA at 298 K.
30. Omichinski, J. G.; Clore, G. M.; Schaad, O.; Felsenfeld, G.; Trainor, C.; Appella, E.; Stahl, S. J.; Gronenborn, A. M. Science 1993, 261, 438-446.
31. Perez-Alvarado, G. C.; Miles, C.; Michelsen, J. W.; Louis, H. A.; Winge, D. R.: Beckerle, M. C.; Summers, M. F. Nat. Struct. Biol. 1994, 1, 388-398.
32. Outten, C. E.; O'Halloran, T. V. Science 2001, 292, 2488-2492.