Identification of amino acid residues critical for catalysis and stability in Aspergillus niger family 1 pectin lyase A

Biochemical Journal

Volumn 370, Issue 1, 2003, Pages 331-337

  Sánchez Torres, Paloma ^a,b   Visser, Jaap ^a,c   Benen, Jacques A E ^a  

^a WAGENINGEN UNIVERSITY   (Netherlands)

^b INSTITUTO DE AGROQUÍMICA Y TECNOLOGÍA DE ALIMENTOS CSIC   (Spain)

^c FGT Consultancy   (Netherlands)

Author keywords

Conformation; Fluorescence; Mutagenesis; Subsite; Three dimensional structure

Indexed keywords

CATALYSIS; ENZYMES; MUTAGENESIS; PH EFFECTS;

AMINO ACID RESIDUES;

AMINO ACIDS;

AMINO ACID; ARGININE; ASPARTIC ACID; FUNGAL ENZYME; GLUTAMINE; LYSINE; MUTANT PROTEIN; PECTIN LYASE; POLYGALACTURONIC ACID; PROTON;

AMINO ACID SUBSTITUTION; ARTICLE; ASPERGILLUS NIGER; CATALYSIS; CIRCULAR DICHROISM; CONFORMATIONAL TRANSITION; CONTROLLED STUDY; ENZYME ASSAY; ENZYME INACTIVATION; ENZYME STABILITY; ENZYME STRUCTURE; ENZYME SUBSTRATE; FLUORESCENCE; NONHUMAN; PH; PRIORITY JOURNAL; PROTEIN FAMILY; PROTEIN INTERACTION; SITE DIRECTED MUTAGENESIS;

AMINO ACIDS; ASPERGILLUS NIGER; BASE SEQUENCE; CATALYSIS; CATALYTIC DOMAIN; CIRCULAR DICHROISM; CLONING, MOLECULAR; DNA PRIMERS; ENZYME STABILITY; HYDROGEN-ION CONCENTRATION; KINETICS; MUTAGENESIS, SITE-DIRECTED; POLYSACCHARIDE-LYASES; PROTEIN CONFORMATION; SPECTROMETRY, FLUORESCENCE;

ASPERGILLUS; ASPERGILLUS NIGER;

EID: 0037443133     PISSN: 02646021     EISSN: None     Source Type: Journal    
DOI: 10.1042/BJ20021071     Document Type: Article

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