A simultaneous colorimetric assay of free and protein-coupled polyethylene glycol

Analytical Biochemistry

Volumn 313, Issue 2, 2003, Pages 335-337

  Li, Shukuan ^a   Yang, Zhijian ^a   Sun, Xinghua ^a   Tan, Yuying ^a   Yagi, Shigeo ^a   Hoffman, Robert M ^a  

^a ANTICANCER INC   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BIOCHEMISTRY;

COLORIMETRIC ASSAYS;

CHEMISTRY;

FLUORESCAMINE; MACROGOL; METHIONINE ALPHA,GAMMA LYASE; RECOMBINANT ENZYME; UNCLASSIFIED DRUG;

ACCURACY; ALKALINITY; COLORIMETRY; CONJUGATION; ENZYME ANALYSIS; ENZYME GLYCOSYLATION; HYDROLYSIS; MATRIX ASSISTED LASER DESORPTION IONIZATION TIME OF FLIGHT MASS SPECTROMETRY; MOLECULAR MODEL; MOLECULAR WEIGHT; NOTE; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; REPRODUCIBILITY;

EID: 0037442723     PISSN: 00032697     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0003-2697(02)00593-6     Document Type: Article

References (5)

1. Nag A., Mitra G., Ghosh P.C. A colorimetric assay for estimation of polyethylene glycol and polyethylene glycolated protein using ammonium ferrothiocyanate. Anal. Biochem. 237:1996;224-231.
2. Tan Y., Xu M., Tan X.-Z., Tan X.-Y., Wang X., Saikawa S., Nagahama T., Sun X., Lenz M., Hoffman R.M. Overexpression and large-scale production of recombinant L-methionine-α-deamino-γ-mercaptomethane-lyase for novel anticancer therapy. Protein Expression Purif. 9:1997;233-245.
3. Tan Y., Sun X., Su M., An Z., Tan X.-Z., Tan X.-Y., Han Q., Miljkovic D.A., Yang M., Hoffman R.M. Polyethylene glycol conjugation of recombinant methioninase for cancer therapy. Protein Expression Purif. 12:1998;45-52.
4. Habeeb A.F. Determination of free amino groups in proteins by trinitrobenzenesulfonic acid. Anal. Biochem. 14:1966;328-336.
5. Stocks S.J., Jones A.J., Ramey C.W., Brooks D.E. A fluorometric assay of the degree of modification of protein primary amines with polyethylene glycol. Anal. Biochem. 154:1986;232-234.