Ara12 subtilisin-like protease from Arabidopsis thaliana: Purification, substrate specificity and tissue localization

Biochemical Journal

Volumn 370, Issue 1, 2003, Pages 57-67

  Hamilton, John M U ^a   Simpson, David J ^b   Hyman, Stefan C ^c   Ndimba, Bongani K ^a   Slabas, Antoni R ^a  

^a DURHAM UNIVERSITY   (United Kingdom)

^b CARLSBERG LABORATORY   (Denmark)

^c UNIVERSITY OF LEICESTER   (United Kingdom)

Author keywords

Apoplast; Pyrolysin; Serine endoprotease

Indexed keywords

BACTERIA; CELL CULTURE; HYDROPHOBICITY; IMMUNOLOGY; RNA; SUSPENSIONS (FLUIDS); TISSUE;

BINDING PROTEIN;

ENZYMES;

ALANINE; ARGININE; LEUCINE; PHENYLALANINE; PROTEIN; PROTEIN ARA12; PROTEINASE; SERINE PROTEINASE INHIBITOR; SUBTILISIN; UNCLASSIFIED DRUG;

ANION EXCHANGE; ARABIDOPSIS; ARTICLE; BACTERIAL CELL; CHROMATOGRAPHY; ENZYME SPECIFICITY; ESCHERICHIA COLI; IMMUNOGOLD LABELING; IMMUNOLOGICAL TECHNIQUE; MORPHOGENESIS; NONHUMAN; PH; PRIORITY JOURNAL; PROTEIN DEGRADATION; PROTEIN EXPRESSION; PURIFICATION; RNA ANALYSIS; THERMOSTABILITY; TISSUE DISTRIBUTION;

ARABIDOPSIS; ARABIDOPSIS PROTEINS; BASE SEQUENCE; BLOTTING, WESTERN; CALCIUM; CHROMATOGRAPHY, GEL; DITHIOTHREITOL; DNA PRIMERS; ELECTROPHORESIS, POLYACRYLAMIDE GEL; HYDROGEN-ION CONCENTRATION; IMMUNOHISTOCHEMISTRY; PROTEASE INHIBITORS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; SPECTROMETRY, MASS, MATRIX-ASSISTED LASER DESORPTION-IONIZATION; SUBSTRATE SPECIFICITY; SUBTILISINS; TEMPERATURE;

ARABIDOPSIS; ARABIDOPSIS THALIANA; BACTERIA (MICROORGANISMS); ESCHERICHIA COLI;

EID: 0037442529     PISSN: 02646021     EISSN: None     Source Type: Journal    
DOI: 10.1042/BJ20021125     Document Type: Article

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