Nonradioactive methods for the assay of phosphoinositide 3-kinases and phosphoinositide phosphatases and selective detection of signaling lipids in cell and tissue extracts

Analytical Biochemistry

Volumn 313, Issue 2, 2003, Pages 234-245

  Gray, Alexander ^a   Olsson, Henric ^b   Batty, Ian H ^a   Priganica, Larisa ^b   Downes, C Peter ^a  

^a UNIVERSITY OF DUNDEE   (United Kingdom)

^b ASTRAZENECA R AND D   (Sweden)

Author keywords

[No Author keywords available]

Indexed keywords

CELLS; CYTOLOGY; DIAGNOSIS; PHOSPHATASES; PHOSPHOLIPIDS; PROBES; TISSUE;

ENZYME CATALYZED REACTION; HIGH THROUGHPUT SCREENING; PHOSPHOINOSITIDE BINDING; PHOSPHOINOSITIDE PHOSPHATASE; PLATELET-DERIVED GROWTH FACTORS; PLECKSTRIN HOMOLOGY DOMAINS; REFERENCE COMPOUNDS; TUMOR SUPPRESSOR PROTEINS;

CELL SIGNALING;

2 MORPHOLINO 8 PHENYLCHROMONE; CELL EXTRACT; FORMYLMETHIONYLLEUCYLPHENYLALANINE; LIPID; PHOSPHATASE; PHOSPHATIDYLINOSITIDE; PHOSPHATIDYLINOSITOL 3 KINASE; PHOSPHATIDYLINOSITOL 3,4,5 TRISPHOSPHATE; PHOSPHATIDYLINOSITOL 3,4,5 TRISPHOSPHATE 3 PHOSPHATASE; PHOSPHOINOSITIDE PHOSPHATASE; PLATELET DERIVED GROWTH FACTOR; STAUROSPORINE; TISSUE EXTRACT; TUMOR SUPPRESSOR PROTEIN; UNCLASSIFIED DRUG; WORTMANNIN;

ACCURACY; ANALYTIC METHOD; ANIMAL CELL; ARTICLE; BINDING AFFINITY; CELL STRAIN 3T3; CELL STRAIN HL 60; CONTROLLED STUDY; ENZYME ASSAY; ENZYME BINDING; HIGH THROUGHPUT SCREENING; HUMAN; HUMAN CELL; IC 50; MOUSE; NONHUMAN; OXIDATIVE STRESS; PRIORITY JOURNAL; PROTEIN ANALYSIS; SCREENING; SEQUENCE HOMOLOGY; TIME RESOLVED FLUORESCENCE RESONANCE ENERGY TRANSFER; VALIDATION PROCESS;

EID: 0037442448     PISSN: 00032697     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0003-2697(02)00607-3     Document Type: Article

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