High-throughput confirmation of differential display PCR results using reverse Northern blotting

Journal of Neuroscience Methods

Volumn 123, Issue 1, 2003, Pages 47-54

  Dilks, Daniel W ^a   Ring, Robert H ^a   Khawaja, Xavier Z ^a   Novak, Thomas J ^a,b   Aston, Christopher ^a  

^a PFIZER INC   (United States)

^b ROCHE BIOSCIENCE   (United States)

Author keywords

Antidepressant drugs; Biomek; Differential display PCR; High throughput; Reverse Northern blot

Indexed keywords

ANTIDEPRESSANT AGENT; DESIPRAMINE; FLUOXETINE; VENLAFAXINE;

ANIMAL CELL; ARTICLE; CELL CULTURE; CLONE; CONTROLLED STUDY; DIFFERENTIAL DISPLAY; DNA MICROARRAY; DNA PURIFICATION; DRUG EFFECT; GENE EXPRESSION; GLIOMA; NONHUMAN; NORTHERN BLOTTING; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; ROBOTICS; TECHNIQUE;

ANIMALS; ANTIDEPRESSIVE AGENTS; ARTIFACTS; BLOTTING, NORTHERN; CARRIER PROTEINS; CELLS, CULTURED; DATA DISPLAY; DRUG EVALUATION, PRECLINICAL; HUMANS; MEMBRANE GLYCOPROTEINS; MEMBRANE TRANSPORT PROTEINS; MOLECULAR SEQUENCE DATA; NERVE TISSUE PROTEINS; NEUROGLIA; NOREPINEPHRINE PLASMA MEMBRANE TRANSPORT PROTEINS; OLIGONUCLEOTIDE ARRAY SEQUENCE ANALYSIS; RATS; RECEPTORS, ADRENERGIC; RECEPTORS, SEROTONIN; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA, MESSENGER; SEROTONIN PLASMA MEMBRANE TRANSPORT PROTEINS; SYMPORTERS; TRANSCRIPTION, GENETIC;

EID: 0037441736     PISSN: 01650270     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0165-0270(02)00343-6     Document Type: Article

References (16)

1. Altschul S.F., Madden T.L., Schaffer A.A., Zhang J., Zhang Z., Miller W., Lipman D.J. Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res. 25:1997;3389-3402.
2. Callard D., Lescure B., Mazzolini L. A method for the elimination of false positives generated by the mRNA differential display technique. Biotechniques. 16:1994;1096-1103.
3. Corton J.C., Gustaffson J.A. Increased efficiency in screening large numbers of cDNA fragments generated by differential display. Biotechniques. 22:1997;802-810.
4. Duman R.S., Malberg J., Thome J. Neural plasticity to stress and antidepressant treatment. Biol. Psychol. 46:1999;1181-1191.
5. Liang P., Pardee A.B. Differential display of eukaryotic messenger RNA by means of the polymerase chain reaction. Science. 257:1992;967-971.
6. Macas J., Nouzova M., Galbraith D.W. Adapting the Biomek 2000 laboratory automation workstation for printing DNA microarrays. Biotechniques. 25:1998;106-110.
7. Mikulits W., Dolznig H., Hofbauer R., Mullner E.W. Reverse strand priming: a versatile cDNA radiolabeling method for differential hybridization on nucleic acid arrays. Biotechniques. 26:1999;846-850.
8. Nestler E.J., Terwilliger R.Z., Duman R.S. Chronic antidepressant administration alters the subcellular distribution of cyclic AMP-dependent protein kinase in rat frontal cortex. J. Neurochem. 53:1989;1644-1647.
9. Poirier G.M., Pyati J., Wan J.S., Erlander M.G. Screening differentially expressed cDNA clones obtained by differential display using amplified RNA. Nucleic Acids Res. 25:1997;913-914.
10. Sambrook J., Fritsch E.F., Maniatis T. Molecular cloning: a laboratory manual. second ed:1989;Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
11. Shieh P.B., Hu S.C., Bobb K., Timmusk T., Ghosh A. Identification of a signaling pathway involved in calcium regulation of BDNF expression. Neuron. 20:1998;727-740.
12. Strakhova M.I., Skolnick P. Can differential display methodologies make an impact on biological psychiatry? Int. J. Neuropsychopharmacol. 4:2001;75-82.
13. Thome J., Sakai N., Shin K.-N., Steffen C., Zhang Y.-J., Impey S., Storm D., Duman R.S. cAMP response element-mediated gene transcription is upregulated by chronic antidepressant treatment. J. Neurosci. 20:2000;4030-4036.
14. Troen B.R., Chauhan S.S., Ray D., Gottesman M.M. Downstream sequences mediate induction of the mouse cathepsin L promoter by phorbol esters. Cell Growth Differ. 2:1991;23-31.
15. Whitney L.W., Becker K.G. Radioactive 33-P probes in hybridization to glass cDNA microarrays using neural tissues. J. Neurosci. Methods. 106:2001;9-13.
16. Zhang H., Zhang R., Liang P. Differential screening of gene expression difference enriched by differential display. Nucleic Acids Res. 24:1996;2425-2454.