Quantitation of CYP1A1 and 1B1 mRNA in polycyclic aromatic hydrocarbon-treated human T-47D and HepG2 cells by a modified bDNA assay using fluorescence detection

Analytical Biochemistry

Volumn 312, Issue 2, 2003, Pages 162-166

  Wu, Susan J ^a   Spink, David C ^a   Spink, Barbara C ^a   Kaminsky, Laurence S ^a  

^a WADSWORTH CENTER   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CELLS; CYTOLOGY; FLUORESCENCE; PHOSPHATASES; PHOTOBLEACHING; POLYCYCLIC AROMATIC HYDROCARBONS; RNA;

ALKALINE PHOSPHATASE; ANALYTICAL SYSTEMS; BENZO[A]PYRENE(B[A]P); ENZYME REGULATION; FLUORESCENCE DETECTION; LABOR INTENSIVE; REVERSE-TRANSCRIPTASE POLYMERASE CHAIN REACTIONS; SIGNAL AMPLIFICATIONS;

POLYMERASE CHAIN REACTION;

2' (2 BENZOTHIAZOL) 6' HYDROXYBENZOTHIAZOLE PHOSPHATE; ALKALINE PHOSPHATASE; ANTHRACENE DERIVATIVE; BENZOTHIAZOLE DERIVATIVE; CYTOCHROME P450 1A1; CYTOCHROME P450 1B1; DIBENZ[A,H]ANTHRACENE; DNA; MESSENGER RNA; POLYCYCLIC AROMATIC HYDROCARBON; RNA; UNCLASSIFIED DRUG;

ARTICLE; BLEACHING; CELL STRAIN HEPG2; CHEMOLUMINESCENCE; CONTROLLED STUDY; DNA DETERMINATION; ENZYME ASSAY; ENZYME INDUCTION; ENZYME REGULATION; ENZYME SUBSTRATE; FLUORESCENCE; FLUOROMETRY; GENE EXPRESSION; GENE TECHNOLOGY; HUMAN; HUMAN CELL; METHODOLOGY; NUCLEIC ACID PROBE; PRIORITY JOURNAL; QUANTITATIVE ANALYSIS; REPRODUCIBILITY; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION;

EID: 0037439497     PISSN: 00032697     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0003-2697(02)00444-X     Document Type: Article

References (11)

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