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note
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2. Hg(II) could not be incorporated using this protocol, but was incorporated using 50 mM sodium borate, 50 mM NaCl, pH 9, followed by exchange into 50 mM Tris/Na, 50 mM NaCl, pH 7.5, metal:protein = 0.8. Cd(II) was not incorporated under either condition.
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-
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14
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0012477548
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note
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3 was added to PcoC in 10 mM HEPES/Na, pH 7.5, 250 mM NaCl, followed by repeated dilution/concentration into 100 mM sodium phosphate, pH 7.2. Final metal:protein = 0.8.
-
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15
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0012381215
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note
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2. The protein concentration 1.8 mM; metal:protein = 0.9.
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16
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0012384404
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2 were freely variable parameters.
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