A possible purification method of DNAs' fragments from humic matters in soil extracts using novel stimulus responsive polymer adsorbent

Journal of Pharmaceutical and Biomedical Analysis

Volumn 30, Issue 6, 2003, Pages 1919-1922

  Hosoya, Ken ^a   Kubo, Takuya ^a   Tanaka, Nobuo ^a   Haginaka, Jun ^b  

^a KYOTO INSTITUTE OF TECHNOLOGY   (Japan)

^b MUKOGAWA WOMEN'S UNIVERSITY   (Japan)

Author keywords

Polymer stationary phases; Purification of DNA; Stimulus responsive adsorbents

Indexed keywords

ADSORBENT; ANION; CATION; DNA; DNA FRAGMENT; POLYMER;

ADSORPTION; ALKALINITY; ANION EXCHANGE; ARTICLE; CATION EXCHANGE; DNA PURIFICATION; EXTRACT; HUMIC SUBSTANCE; METHODOLOGY; PH; PRIORITY JOURNAL; PRODUCT RECOVERY; ROOM TEMPERATURE; SOIL; SOIL ANALYSIS; STIMULUS RESPONSE; TEMPERATURE DEPENDENCE; ISOLATION AND PURIFICATION; TEMPERATURE;

ADSORPTION; DNA; HUMIC SUBSTANCES; POLYMERS; SOIL; TEMPERATURE;

EID: 0037437709     PISSN: 07317085     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0731-7085(02)00535-6     Document Type: Article

References (8)

1. Torsvik V., Goksøyr J., Daae F.L. High diversity in DNA of soil bacteria. Appl. Environ. Microbiol. 56:1990;782-787.
2. Torsvik V., Salte K., Sørheim R., Goksøyr J. Comparison of phenotypic diversity and DNA heterogeneity in a population of soil bacteria. Appl. Environ. Microbiol. 56:1990;776-781.
3. Fægri A., Torsvik V.L., Goksöyr J. Bacterial and fungal activities in soil: separation of bacteria and fungi by a rapid fractionated centrifugation technique. Soil Biol. Biochem. 9:1977;105-112.
4. Torsvik V.L., Goksøyr J. Determination of bacterial DNA in soil. Soil Biol. Biochem. 10:1978;7-12.
5. Jurcsik I. Humic Substances in the Global Environmental and Implication Human Health. 1994;1331 Elsevier.
6. Yoshizako K., Hosoya K., Kimata K., Araki T., Tanaka N. A polymeric surface functionality immobilized on uniformly sized macroporous polymer beads. J. Polym. Sci. Part A. 35:1997;2747-2757.
7. Smigol V., Svec F., Hosoya K., Wang Q., Fréchet J.M.J. Monodisperse polymer beads as packing material for high-performance liquid chromatography. Angew. Makromol. Chem. 195:1992;151-164.
8. Okano T. Stimulus responsive drug delivery system (DDS). Farumashia. 29:1993;1276-1281.