An improved 16S rRNA based PCR method for the specific detection of Salmonella enterica

International Journal of Food Microbiology

Volumn 80, Issue 1, 2003, Pages 67-75

  Trkov, Marija ^a   Avguštin, Gorazd ^a  

^a UNIVERSITY OF LJUBLJANA   (Slovenia)

Author keywords

16S rRNA; Molecular detection; PCR; Salmonella

Indexed keywords

RNA 16S;

ARTICLE; BACTERIAL STRAIN; BACTERIUM DETECTION; CONTROLLED STUDY; ENTEROBACTER CLOACAE; NONHUMAN; POLYMERASE CHAIN REACTION; RNA SEQUENCE; SALMONELLA ENTERICA; SEQUENCE ANALYSIS; SEROTYPE; STRAIN DIFFERENCE;

BASE SEQUENCE; DNA PRIMERS; DNA, BACTERIAL; FOOD MICROBIOLOGY; POLYMERASE CHAIN REACTION; RNA, RIBOSOMAL, 16S; SALMONELLA ENTERICA; SENSITIVITY AND SPECIFICITY; SEQUENCE ALIGNMENT; SEQUENCE HOMOLOGY, NUCLEIC ACID;

ENTEROBACTER; ENTEROBACTER CLOACAE; PROTEOBACTERIA; SALMONELLA; SALMONELLA BONGORI; SALMONELLA ENTERICA;

EID: 0037437439     PISSN: 01681605     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0168-1605(02)00138-1     Document Type: Article

References (20)

1. Aabo S., Rasmussen O.F., Rossen L., Sorensen P.D., Olsen J.E. Salmonella identification by the polymerase chain reaction. Mol. Cell. Probes. 7:1993;171-178.
2. Amann R.I., Ludwig W., Schleifer K.H. Phylogenetic identification and in situ detection of individual microbial cells without cultivation. Microbiol. Rev. 59:1995;143-169.
3. Baird-Parker A.C. Foodborne illness. Foodborne salmonellosis. Lancet. 336:1990;1231-1235.
4. Davey H.M., Kell D.B. Flow cytometry and cell sorting of heterogeneous microbial populations: the importance of single-cell analysis. Microbiol. Rev. 60:1996;641-696.
5. Fogel G.B., Collins C.R., Li J., Brunk C.F. Prokaryotic genome size and ssu rDNA copy number: estimation of microbial relative abundance from a mixed population. Microb. Ecol. 38:1999;93-113.
6. Higgins D.G., Bleasby A.J., Fuchs R. Clustal V: improved software for multiple sequence alignment. CABIOS. 8:1992;189-191.
7. Lane D.J. 16S/23S rRNA sequencing. Stackebrant E., Goodfellov M. Nucleic Acids Techniques in Bacterial Systematics. 1991;115-148 Wiley, Chichester.
8. Le Minor L., Popoff M.Y. Designation of Salmonella enterica sp. nom. rev., as the type and only species of the genus Salmonella. Int. J. Syst. Bacteriol. 37:1987;465-468.
9. Lewis M.J. Salmonella. Greenwood D., Slack R.C.B., Peutherer J.F. Medical Microbiology. 15th edn. 1997;252-261 Churchill Livingstone, London.
10. Lin C.K., Tsen H.Y. Development and evaluation of two novel oligonucleotide probes based on 16S rRNA sequence for the identification of Salmonella in foods. J. Appl. Bacteriol. 78:1995;507-520.
11. Lin C.K., Tsen H.Y. Use of two 16S DNA targeted oligonucleotides as PCR primers for the specific detection of Salmonella in foods. J. Appl. Bacteriol. 80:1996;659-666.
12. Maidak B.L., Cole J.R., Lilburn T.G., Parker C.T. Jr., Saxman P.R., Stredwick J.M., Garrity G.M., Li B., Olsen G.J., Pramanik S., Schmidt T.M., Tiedje J.M. The RDP (Ribosomal Database Project) continues. Nucleic Acids Res. 28:2000;173-174.
13. Olsen G.J., Lane D.J., Giovannoni S.J., Pace N.R., Stahl D.A. Microbial ecology and evolution: a ribosomal RNA approach. Annu. Rev. Microbiol. 40:1986;337-365.
14. Olsen J.E., Aabo S., Nielsen E.O., Nielsen B.B. Isolation of a Salmonella-specific DNA hybridization probe. APMIS, Acta Pathol. Microbiol. Immunol. Scand. 99:1991;114-120.
15. Olsen J.E., Aabo S., Hill W., Notermans D., Wernars K., Granum P.E., Popovic T., Rasmussen P.E., Olsvik O. Probes and polymerase chain reaction for detection of food-borne bacterial pathogenes. Int. J. Food Microbiol. 28:1995;1-78.
16. Scheu P.M., Berghof K., Stahl U. Detection of pathogenic and spoilage micro-organisms in food with the polymerase chain reaction. Food Microbiol. 15:1998;13-31.
17. Tauxe R. Salmonella: a postmodern pathogen. J. Food Prot. 54:1991;563-568.
18. Thomson J.D., Higgins D.G., Gibson T.J. Clustal W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, positions-specific gap penalties and weight matrix choice. Nucleic Acids Res. 22:1994;4673-4680.
19. Trkov M., Majerikova I., Jeršek B., Stefanovicova A., Rijpens N., Kuchta T. Detection of Salmonella in food in 30 hours using enrichment and polymerase chain reaction. Food Microbiol. 16:1999;393-399.
20. Weisburg W.G., Barns S.M., Pelletier D.A., Lane D.J. 16S ribosomal DNA amplification for phylogenetic study. J. Bacteriol. 173:1991;697-703.