Mutagenesis of cysteine 280 of the reverse transcriptase of human immunodeficiency virus type-1: The effects on the ribonuclease H activity

Journal of Molecular Biology

Volumn 327, Issue 1, 2003, Pages 19-30

  Sevilya, Ziv ^a   Loya, Shoshana ^a   Duvshani, Amit ^a   Adir, Noam ^b   Hizi, Amnon ^a  

^a TEL AVIV UNIVERSITY   (Israel)

^b TECHNION ISRAEL INSTITUTE OF TECHNOLOGY   (Israel)

Author keywords

DNA polymerase; HIV 1; Reverse transcriptase; RNase H

Indexed keywords

CYSTEINE; PROTEIN P51; PROTEIN P66; PROTEIN SUBUNIT; RIBONUCLEASE H; RNA DIRECTED DNA POLYMERASE; UNCLASSIFIED DRUG;

ARTICLE; CONTROLLED STUDY; DNA STRAND; ENZYME ACTIVITY; HUMAN IMMUNODEFICIENCY VIRUS 1; KINETICS; MICHAELIS CONSTANT; MUTAGENESIS; NONHUMAN; PRIORITY JOURNAL; STEADY STATE;

HUMAN IMMUNODEFICIENCY VIRUS; HUMAN IMMUNODEFICIENCY VIRUS 1;

EID: 0037436406     PISSN: 00222836     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0022-2836(03)00052-4     Document Type: Article

References (32)

1. Coffin J.M., Hughes S.H., Varmus H.E. Retroviruses. 1997;Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
2. Goff S.P. Retroviral reverse transcriptases: synthesis, structure, and function. J. AIDS. 3:1990;817-831.
3. Skalka A.M., Goff S.P. Reverse Transcriptase. 1993;Cold Spring Harbor Laboratory, Cold Spring Harbor, NY.
4. De Clercq E. New developments in anti-HIV chemotherapy. Biochem. Biophys. Acta. 1587:2002;258-275.
5. Veronese F.D., Copeland T.D., Devico A.L., Rahman R., Gallo R.C. Characterization of highly immunogenic p66/p51 as the reverse transcriptase of HTLV-III/LAV. Science. 231:1986;1291-1298.
6. Kohlstaedt L.A., Wang J., Friedman J.M., Rice P.A., Steitz T.A. Crystal structure at 3.5 Å resolution of HIV-1 reverse transcriptase complexed with an inhibitor. Science. 256:1992;1783-1790.
7. Jacobo-Molina A., Ding J., Nami R.G., Clark A.D.J., Lu X., Tantillo C., et al. Crystal structure of human immunodeficiency virus type 1 reverse transcriptase complexed with double-stranded DNA at 3.0 Å resolution shows bent DNA. Proc. Natl. Acad. Sci. USA. 90:1993;6230-6234.
8. Huang H., Chopra R., Verdine G.L., Harrison S.C. Structure of a covalently-trapped catalytic complex of HIV-1 reverse transcriptase: implications for drug resistance. Science. 282:1998;1669-1675.
9. Ding J., Das K., Hsiou Y., Sarafianos S.G., Clark A.G., Jacobo-Molina A., et al. Structure and functional implications of the polymerase active site region in a complex of HIV-1 RT with a double stranded DNA template-primer and an antibody Fab fragment at 2.8 Å resolution. J. Mol. Biol. 284:1998;1095-1111.
10. Georgiadis M.M., Jessen S.M., Ogata C.M., Telesnitsky A., Goff S.P., Hendrickson W.A. Mechanistic implications from the structure of a catalytic fragment of Moloney murine leukemia virus reverse transcriptase. Structure. 3:1995;879-892.
11. Sawaya M.R., Pelletier H., Kumar A., Wilson S.H., Kraut J. Crystal structure of rat DNA polymerase β. Evidence for a common polymerase mechanism. Science. 264:1994;1930-1935.
12. Ollis D.L., Brick P., Hamlin R., Xunong N.G., Steitz T.A. Structure of large fragment of Escherichia coli DNA polymerase I complexed with dTMP. Nature. 313:1985;762-766.
13. Jeruzalmi D., Steitz T.A. Structure of T7 RNA polymerase complexed to the transcriptional inhibitor T7 lysozyme. EMBO J. 17:1998;4101-4113.
14. Li Y., Korolev S., Waksman K. Crystal structures of open and closed forms of binary and ternary complexes of the large fragment of Thermus aquaticus DNA polymerase I: structural basis for nucleotide incorporation. EMBO J. 17:1998;7514-7525.
15. Doublie S., Tabor S., Long A.M., Richardson C.C., Ellenberger T. Crystal structure of a bacteriophage T7 DNA replication complex at 2.2 Å resolution. Nature. 391:1998;251-258.
16. Hansen J.L., Long A.M., Schultz S.C. Structure of the RNA-dependent RNA polymerase of poliovirus. Structure. 5:1997;1109-1122.
17. Hizi A., McGill C., Hughes S.H. Expression of soluble, enzymatically active, human immunodeficiency virus reverse transcriptase in Escherichia coli and analysis of mutants. Proc. Natl Acad. Sci. USA. 85:1988;1218-1222.
18. Hizi A., Tal R., Hughes S.H. Mutational analysis of the DNA polymerase and ribonuclease H activities of human immunodeficiency virus type 2 reverse transcriptase expressed in Escherichia coli. Virology. 180:1991;339-346.
19. Jacobo-Molina A., Arnold E. HIV reverse transcriptase structure-function relationships. Biochemistry. 30:1991;6351-6356.
20. Shaharabany M., Hizi A. The catalytic functions of chimeric reverse transcriptases of human immunodeficiency viruses type 1 and type 2. J. Biol. Chem. 267:1992;3647-3678.
21. Sevilya Z., Loya S., Hughes S.H., Hizi A. The ribonuclease H activity of the reverse transcriptase of human immunodeficiency viruses type 1 and type 2 is affected by the thumb subdomain of the small subunits. J. Mol. Biol. 311:2001;957-971.
22. Sevilya Z., Loya S., Adir N., Hizi A. The ribonuclease H activity of the reverse transcriptases of human immunodeficiency viruses type-1 and type-2 is modulated by residue 294 of the small subunit. Nucl. Acids Res. 31:2003;. In press.
23. Hizi A., Tal R., Shaharabany M., Loya S. Catalytic properties of the reverse transcriptases of human immunodeficiency viruses type 1 and type 2. J. Biol. Chem. 266:1991;6230-6239.
24. Hizi A., Shaharabany M., Tal R., Hughes S.H. The effects of cysteine mutations on the reverse transcriptases of human immunodeficiency virus types 1 and 2. J. Biol. Chem. 267:1992;1293-1297.
25. Loya S., Tal R., Hizi A., Hughes S.H. The effects of cysteine mutations on the catalytic activities of the reverse transcriptase of human immunodeficiency virus type-1. J. Biol. Chem. 267:1992;13879-13883.
26. Loya S., Gao H.-Q., Avidan O., Boyer P.L., Hughes S.H., Hizi H. Subunit-specific mutagenesis of the cysteine 280 residue of the reverse transcriptase of human immunodeficiency virus type 1: effects on sensitivity to a specific inhibitor of the RNase H activity. J. Virol. 71:1997;5668-5672.
27. Gao H.Q., Sarafianos S.G., Arnold E., Hughes S.H. Similarities and differences in the RNase H activities of human immunodeficiency virus type 1 reverse transcriptase and Moloney murine leukemia virus reverse transcriptase. J. Mol. Biol. 294:1999;1097-1113.
28. Wisniewski M., Balakrishan M., Palaniappan C., Fay P.J., Bambara R.A. Unique progressive cleavage mechanism of HIV reverse transcriptase RNase H. Proc. Natl Acad. Sci. USA. 97:2000;11978-11983.
29. Palaniappan C., Fuentes G.M., Rodriguez-Rodriguez L., Fay P.J., Bambara R.A. Helix structure and ends of RNA/DNA hybrids direct the cleavage specificity of HIV-1 reverse transcriptase RNase H. J. Biol. Chem. 271:1996;2063-2070.
30. Boyer P.L., Ferris A.L., Hughes S.H. Cassette mutagenesis of the reverse transcriptase of human immunodeficiency virus type 1. J. Virol. 66:1992;1031-1039.
31. Hizi A., Hughes S.H., Shaharabany M. Mutational analysis of the ribonuclease H activity of human immunodeficiency virus 1 reverse transcriptase. Virology. 175:1990;575-580.
32. Sarafianos S.G., Das K., Tantillo C., Clark A.D. Jr, Ding J., Whitcomb J., et al. Crystal structure of HIV-1 reverse transcriptase in complex with a polypurine tract RNA·DNA. EMBO J. 20:2001;1449-1461.