The KlFUS1 gene is required for proper haploid mating and its expression is enhanced by the active form of the Gα (Gpa1) subunit involved in the pheromone response pathway of the yeast Kluyveromyces lactis

FEMS Microbiology Letters

Volumn 219, Issue 1, 2003, Pages 105-113

  Lloret, Alejandro ^a   Saviñón Tejeda, Alma ^a   Ongay Larios, Laura ^a   Tenorio, Eda P ^a   Coria, Roberto ^a  

^a INSTITUTO DE FISIOLOGÍA CELULAR   (Mexico)

Author keywords

Fus1; G protein; Kluyveromyces lactis; Mating; Signal transduction

Indexed keywords

GUANINE NUCLEOTIDE BINDING PROTEIN; HYBRID PROTEIN; MEMBRANE PROTEIN; PROTEIN SUBUNIT; SERINE; SEX PHEROMONE; THREONINE; TRANSCRIPTION FACTOR;

AMINO ACID SEQUENCE; AMINO TERMINAL SEQUENCE; ARTICLE; CARBOXY TERMINAL SEQUENCE; CELL DIVISION; CELL ELONGATION; CELL MEMBRANE; CONJUGATION; CYTOPLASM; FUNGAL STRAIN; GENE; GENE EXPRESSION; GENE OVEREXPRESSION; GENETIC CODE; HAPLOIDY; KLUYVEROMYCES; MATING SYSTEM; MOLECULAR CLONING; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROTEIN GLYCOSYLATION; PROTEIN LOCALIZATION; PROTEIN STRUCTURE; SACCHAROMYCES CEREVISIAE;

KLUYVEROMYCES LACTIS; SACCHAROMYCES CEREVISIAE;

EID: 0037436088     PISSN: 03781097     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0378-1097(02)01202-8     Document Type: Article

References (43)

1. Gustin M.C., Alberyn J., Alexander M., Davenport K. MAP kinase pathways in the yeast Saccharomyces cerevisiae. Microbiol. Mol. Biol. Rev. 62:1998;1264-1300.
2. Dietzel C., Kurjan J. The yeast SCG1 gene: a Gα-like protein implicated in the a- and α-factor response pathway. Cell. 50:1987;1001-1010.
3. Whiteway M., Hougan L., Dignard D., Thomas D.Y., Bell L., Saari G.C., Grant F.J., O'Hara P., MacKay V.L. The Ste4 and Ste18 genes of yeast encode potential β and γ subunits of the mating factor receptor-coupled G protein. Cell. 56:1989;467-477.
4. Herskowitz I. MAP kinase pathways in yeast: for mating and more. Cell. 80:1995;187-197.
5. Dolan J.W., Kirkman C., Fields S. The yeast Ste12 protein binds to the DNA sequence mediating pheromone induction. Proc. Natl. Acad. Sci. USA. 86:1989;5703-5707.
6. Kronstad J.W., Holly J.A., Mackay V.L. A yeast operator overlaps an upstream activation site. Cell. 50:1987;369-377.
7. Van Arsdell, S.W. and Thorner, J. (1987) Hormonal regulation of gene expression in yeast. In: Transcriptional Control Mechanism (Granner, D., Rosenfeld, M.G. and Chang, S., Eds.), pp. 325-332. Alan R. Liss, New York.
8. Dolan J.W., Fields S. Overproduction of the yeast Ste12 protein leads to constitutive transcriptional induction. Genes Dev. 4:1990;492-502.
9. Song O., Dolan J.W., Yuan Y.O., Fields S. Pheromone-dependent phosphorylation of the yeast STE12 protein correlates with transcriptional activation. Genes Dev. 5:1991;741-750.
10. Jarvis E.E., Clark K.L., Sprague G.F. The yeast transcription factor PRFTF, a homolog of the mammalian serum response factor, is encoded by the MCM1 gene. Genes Dev. 3:1989;936-945.
11. Yuan Y.O., Stroke I.L., Fields S. Coupling of cell identity to signal response in yeast: interaction between the α1 and Ste12 proteins. Genes Dev. 7:1993;1584-1597.
12. Jackson C.L., Hartwell L.H. Courtship in S. cerevisiae: both cell types choose mating partners by responding to the strongest pheromone signal. Cell. 63:1990;1039-1051.
13. Valtz N., Peter M., Herskowitz J. Far1 is required for oriented polarization of yeast cells in response to mating pheromones. J. Cell Biol. 128:1995;779-792.
14. Brizzio V., Gammie A.E., Nijbroek G., Michaelis S., Rose M.D. Cell fusion during yeast mating requires high levels of a-factor mating pheromone. J. Cell Biol. 135:1996;1727-1739.
15. Berlin V., Brill J., Trueheart J., Boeke J., Fink G.R. Genetic screens and selections for cell and nuclear fusion mutants. Methods Enzymol. 194:1991;774-792.
16. Elion E.A., Grisafi P.L., Fink G.R. Fus3 encodes a cdc2/CDC28 related kinase required for the transition from mitosis into conjugation. Cell. 60:1990;649-664.
17. Trueheart J., Boeke J.D., Fink G.R. Two genes required for cell fusion during yeast conjugation: evidence for a pheromone-induced surface protein. Mol. Cell. Biol. 7:1987;2316-2328.
18. Bardwell L., Cook J.G., Inouye J.C., Thorner J. Signal propagation and regulation in the mating pheromone response pathway of the yeast Saccharomyces cerevisiae. Dev. Biol. 166:1994;363-379.
19. Elion E.A., Satterberg B., Kranz J.E. FUS3 phosphorilates multiple components of the mating signal transduction cascade: evidence for STE12 and FAR1. Mol. Biol. Cell. 4:1993;495-510.
20. Saviñón-Tejeda A., Ongay-Larios L., Valdés-Rodríguez J., Coria R. The KlGpa1 gene encodes a G-protein α subunit that is a positive control element in the mating pathway of the budding yeast Kluyveromyces lactis. J. Bacteriol. 183:2001;229-234.
21. Brake A., Irvine B., Masiarz F., Shultz K. Structure of genes encoding precursors of two Kluyveromyces lactis transported proteins. Yeast. 4:1988;S436.
22. Bolotin-Fukuhara M., Toffano-Nioche C., Artiguenave F., Duchateau-Nguyen G., Lemaire M., Marmeisse R., Montrocher R., Robert C., Termier M., Wincker P., Wesolowski-Louvel M. Genomic exploration of the hemiascomycetous yeast: 11. Kluyveromyces lactis. FEBS Lett. 487:2000;66-70.
23. Saviñón-Tejeda A., Ongay-Larios L., Ramírez J., Coria R. Isolation of a gene encoding a G protein α subunit involved in the regulation of cAMP levels in the yeast Kluyveromyces lactis. Yeast. 12:1996;1125-1133.
24. Wach A., Brachat A., Pohlmann R., Philippsen P. New heterologous modules for classical or PCR-based gene disruptions in Saccharomyces cerevisiae. Yeast. 10:1994;5251-5258.
25. Ongay-Larios L., Saviñón-Tejeda A., Williamson M.J. Jr., Durán-Avelar M.J., Coria R. The Leu-132 of the Ste4(Gβ) subunit is essential for proper coupling of the G protein with the Ste2 α factor receptor during the mating pheromone response in yeast. FEBS Lett. 467:2000;22-26.
26. Sambrook, J., Fritsch, E.F. and Maniatis, T. (1989) Molecular Cloning: A Laboratory Manual, 2nd edn. Cold Spring Harbor Laboratory Press, Plainview, NY.
27. Sherman, F., Fink, G.R. and Hicks, J. (1986) Methods in Yeast Genetics. Cold Spring Harbor Laboratory Press, Plainview, NY.
28. Amberg D.C., Basart E., Botstein D. Defining protein interactions with yeast actin in vivo. Nat. Struct. Biol. 2:1995;28-35.
29. Petersen J., Weilguny D., Egel R., Nielsen O. Characterization of fus1 of Schizosaccharomyces pombe: a developmentally controlled function needed for conjugation. Mol. Cell. Biol. 15:1995;3697-3707.
30. Kyte J., Doolittle R.F. A simple method for displaying the hydropathic character of a protein. J. Mol. Biol. 157:1982;105-132.
31. Trueheart J., Fink G.R. The yeast cell fusion protein FUS1 is O-glycosylated and spans the plasma membrane. Proc. Natl. Acad. Sci. USA. 86:1989;9916-9920.
32. McCaffrey G., Clay F.J., Kelsay K., Sprague G.F. Jr. Identification and regulation of a gene required for cell fusion during mating of the yeast Saccharomyces cerevisiae. Mol. Cell. Biol. 7:1987;2680-2690.
33. Elion E.A., Trueheart J., Fink G.R. Fus2 localizes near the site of cell fusion and is required for both cell fusion and nuclear alignment during zygote formation. J. Cell Biol. 130:1995;1283-1296.
34. Wésolowski-Louvel, M., Breunig, K.D. and Fukuhara, H. (1996) Kluyveromyces lactis: Genetics, Biochemistry and Molecular Biology of Non-conventional Yeast. Springer-Verlag, Berlin.
35. Dohlman H.G., Song J., Ma D., Courchesne W.E., Thorner J. Sst2, a negative regulator of pheromone signaling in the yeast Saccharomyces cerevisiae: expression, localization, and genetic interaction and physical association with Gpa1 (the G-protein alpha subunit). Mol. Cell. Biol. 16:1996;5194-5209.
36. Dohlman H.G. G proteins and pheromone signaling. Annu. Rev. Physiol. 64:2002;129-152.
37. Rodaway A.R.F., Sternberg M.J.E., Bentley D.L. Similarity in membrane proteins. Nature. 342:1989;624.
38. Mayer B.J., Baltimore D. Signaling through SH2 and SH3 domains. Trends Cell Biol. 3:1993;8-13.
39. Hagen D.C., McCaffrey G., SpragueJr G.F. Pheromone response elements are necessary and sufficient for basal and pheromone-induced transcription of the FUS1 gene of Saccharomyces cerevisiae. Mol. Cell. Biol. 11:1991;2952-2961.
40. Gammie A.E., Brizzio V., Rose M.D. Distinct morphological phenotypes of cell fusion mutants. Mol. Biol. Cell. 9:1998;1395-1410.
41. Llorente B., Durrens P., Malpertuy A., Aigle M., et al. Genomic exploration of the Hemiascomycetous yeast: 20. Evolution of gene redundancy compared to Saccharomyces cerevisiae. FEBS Lett. 487:2000;122-133.
42. Lengeler K.B., Davidson R.C., D'Souza C., Harashima T., Shen W.-C., Wang P., Pan X., Waugh M., Heitman J. Signal transduction cascades regulating fungal development and virulence. Microbiol. Mol. Biol. Rev. 64:2000;746-785.
43. Obara T., Nakafuku M., Yamamoto M., Kaziro Y. Isolation and characterization of a gene encoding a G-protein alpha subunit from Schizosaccharomyces pombe: involvement in mating and sporulation pathways. Proc. Natl. Acad. Sci. USA. 88:1991;5877-5881.