Kinetic locking-on and auxiliary tactics for bioaffinity purification of NADP+-dependent dehydrogenases using N6-linked immobilized NADP+ derivatives: Studies with mammalian and microbial glutamate dehydrogenases

Biotechnology and Bioengineering

Volumn 81, Issue 3, 2003, Pages 356-369

  McMahon, Mary ^a   Tynan, Julie ^a   Mulcahy, Patricia ^a  

^a South East Technological University   (Ireland)

Author keywords

Bovine liver; Candida utilis; Glucose 6 phosphate dehydrogenase; Glutamate dehydrogenase; Kinetic locking onstrategy; N6 linked immobilized NADP+

Indexed keywords

CELLS; CHROMATOGRAPHY; ENZYME KINETICS; ENZYMES; EXTRACTION; PURIFICATION;

BIOAFFINITY;

BIOTECHNOLOGY;

ADENOSINE DIPHOSPHATE; ALKALINE PHOSPHATASE; CELL EXTRACT; GLUTAMATE DEHYDROGENASE; LIGAND; NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE; NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE DERIVATIVE; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE DEHYDROGENASE; UNCLASSIFIED DRUG;

AFFINITY CHROMATOGRAPHY; ANIMAL TISSUE; ARTICLE; CANDIDA UTILIS; CATTLE; ELECTROPHORESIS; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME ASSAY; ENZYME INHIBITION; ENZYME PURIFICATION; ENZYME SPECIFICITY; ENZYME SYNTHESIS; NONHUMAN; PROTEIN DETERMINATION; SYNTHESIS; TECHNIQUE;

ANIMALS; CANDIDA; CATTLE; CHROMATOGRAPHY, AFFINITY; ENZYME ACTIVATION; ENZYMES, IMMOBILIZED; GLUTAMATE DEHYDROGENASE; GLUTAMATE DEHYDROGENASE (NADP+); LIVER; NADP; SPECIES SPECIFICITY;

BOVINAE; CANDIDA; MAMMALIA; PICHIA JADINII;

EID: 0037419703     PISSN: 00063592     EISSN: None     Source Type: Journal    
DOI: 10.1002/bit.10547     Document Type: Article

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