A specific method for determination of pectin esterase in blood oranges

Enzyme and Microbial Technology

Volumn 32, Issue 1, 2003, Pages 174-177

  Spagna, Giovanni ^a   Barbagallo, Riccardo N ^a   Ingallinera, Barbara ^a  

^a UNIVERSITY OF CATANIA   (Italy)

Author keywords

Blood oranges; Enzyme properties; Pectin esterase determination

Indexed keywords

ASSAYS; ENZYMES; EXTRACTION; PH EFFECTS; SURFACE ACTIVE AGENTS;

CITRUS JUICES;

FRUIT JUICES;

ESTERASE; SURFACTANT;

ARTICLE; ORANGE (FRUIT); PH; PHYSICAL EXAMINATION; PROTEIN DETERMINATION; TECHNIQUE;

CITRUS;

EID: 0037413382     PISSN: 01410229     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0141-0229(02)00271-5     Document Type: Article

References (11)

1. Pilnik W, Voragen AGJ. Enzymes in Food Processing. 3rd Ed. London, UK: Academic Press; 1993, p. 363-93.
2. Wong DWS. Food Enzymes: Structure and Mechanism. 1st Ed. New York: Chapman & Hall; 1995, p. 7-125.
3. Pennisi L. Trattato di Agrumicoltura, Edagricole. 1st ed. Italy: BO; 1985, p. 481-542.
4. Phaff H.J. α-1,4-Polygalacturonide glycanohyrolase (endo-polygalacturonase) from Saccharomyces fragilis. Methods Enzymol. 8:1966;628-641.
5. Hagerman A.E., Austin P.J. Continuos spectrophotometric assay for plant pectin methylesterase. J. Agric. Food Chem. 34:1986;440-444.
6. Kertesz ZI. The pectic substances. 1st ed. New York: Interscience-Wiley; 1951, p. 118.
7. Wood P.J., Siddiqui I.R. Determination of methanol and its application to measurement of pectic ester content and pectic methyl esterase activity. Anal. Biochem. 39:1971;418-428.
8. Pifferi P.G., Malacarne A., Lanzarini G., Casoli U. Spectrophotometric method for the determination of pectin methylesterase activity by Besthorn's hydrazone. Chem. Mikrobiol. Technol. Lebensm. 9:1985;65-69.
9. Pays M., Malangeau P., Bourdon R. Dosage colorimétrique de divers aldéhydes en présence de l'hydrazone de la 3-méthylbenzothiazoline 2-one. Ann. Pharm. Fr. 24:1966;763-768.
10. Kohn R., Markovic O., Machova E. Deesterification mode of pectin by pectin esterases of Aspergillus foetidus, tomatoes and alfalfa. Col. Czech. Chem. Comm. 48:1983;791-797.
11. Günata Z., Blodeel C., Vallier M.J., Lepoutre J.I., Sapise J.C., Watanabe N. An endoglycosidase from grape berry shin of cv. M. alexandria hydrolyzing potentially aromatic bisaccharide glycosides. J. Agric. Food Chem. 46:1998;2748-2753.