Does 13C-or 15N-labeling affect Cu(I)-thiolate cluster arrangement in yeast copper-metallothionein?

BioMetals

Volumn 16, Issue 3, 2003, Pages 379-382

  Hartmann, Hans Jürgen ^a   Kaup, Yoka ^a   Weser, Ulrich ^a  

^a UNIVERSITY OF TÜBINGEN   (Germany)

Author keywords

13C 15N labeling; Cu(I) thiolate cluster; Yeast Cu(I) metallothionein

Indexed keywords

CADMIUM; CARBON 13; COPPER DERIVATIVE; COPPER THIOLATE; METALLOTHIONEIN; NITROGEN 15; THIOL DERIVATIVE; UNCLASSIFIED DRUG; ZINC; CARBON; CARRIER PROTEIN; COPPER; COPPER METALLOTHIONEIN; CUP1 1 PROTEIN, S CEREVISIAE; CUP1-1 PROTEIN, S CEREVISIAE; ISOTOPE; NITROGEN;

ARTICLE; CHEMICAL STRUCTURE; CIRCULAR DICHROISM; CONTROLLED STUDY; CULTURE MEDIUM; FUNGUS GROWTH; ISOTOPE LABELING; LUMINESCENCE; NONHUMAN; PROTEIN ISOLATION; SACCHAROMYCES CEREVISIAE; SPECTROSCOPY; STRUCTURE ANALYSIS; YEAST CELL; ATOMIC ABSORPTION SPECTROMETRY; CHEMISTRY; ISOLATION AND PURIFICATION; METABOLISM; SPECTROPHOTOMETRY;

FUNGI; SACCHAROMYCES; SACCHAROMYCES CEREVISIAE;

CARBON ISOTOPES; CARRIER PROTEINS; CIRCULAR DICHROISM; COPPER; ISOTOPES; LUMINESCENT MEASUREMENTS; METALLOTHIONEIN; NITROGEN ISOTOPES; SACCHAROMYCES CEREVISIAE; SPECTROPHOTOMETRY; SPECTROPHOTOMETRY, ATOMIC;

EID: 0037412021     PISSN: 09660844     EISSN: None     Source Type: Journal    
DOI: 10.1023/A:1022508825125     Document Type: Article

References (10)

1. Atlas RM. 1993 In: Parks LC., ed. Handbook of Microbiological Media. Boca Raton: CRC Press.
2. 7-metallothionein. Eur J Biochem 267, 1008-1018.
3. Byrd J, Berger RM, McMillin DR, Wright CF, Hamer D, Winge DR. 1988 Characterization of the copper-thiolate cluster in yeast metallothionein and two truncated mutants. J Biol. Chem 263, 6688-6694.
4. Hathaway BF. 1987 In: Wilkinson G, Gillard RD, McCleverty JA eds. Comprehensive coordination chemistry (late transition elements) The synthesis reactions, properties & applications of coordination compounds. Oxford: Pergamon Press; Vol. 5(53); 533-774.
5. Narula SS, Mehra RK, Winge DR, Armitage IM. 1991 Establishment of the metal-to- cysteine connectivities in silver-substituted yeast metallothionein. J. Am. Chem. Soc. 113, 9354-9358.
6. 1H NMR assignments reflecting conformational heterogeneity at the C terminus. Biochemistry 32, 6773-6787.
7. Peterson CW, Narula SS, Armitage IM. 1996 3D solution structure of copper and silver-substituted yeast metallothioneins. FEBS Lett. 379, 85-93.
8. Weser U, Hartmann H-J. 1991 The purification of yeast copper-metallothionein. Methods Enzymol. 205, 274-278.
9. Winge DR, Nielson DB, Gray WR, Hamer DH. 1985 Yeast metallothionein. Sequence and metal-binding properties. J. Biol. Chem. 260, 14464-14470.
10. Zhu XK, Guo Y, Williams RJP, O'Nions RK, Matthews A, Belshaw NS, Canters GW, de Waal EC, Weser U, Burgess BK, Salvato B. 2002 Mass fractionation processes of transition metal isotopes. Earth Planet. Sci. Lett. 200, 47-62.