Application of crossover-PCR-mediated deletion-insertion mutagenesis to analysis of the bdhA-xdhA2-xdhB2 mixed-function operon of Sinorhizobium meliloti

Archives of Microbiology

Volumn 179, Issue 4, 2003, Pages 301-304

  Sukdeo, Nicole ^a   Charles, Trevor C ^a  

^a UNIVERSITY OF WATERLOO   (Canada)

Author keywords

Crossover PCR; PHB cycle; Purine degradation

Indexed keywords

3 HYDROXYBUTYRATE DEHYDROGENASE; XANTHINE OXIDASE;

ARTICLE; BACTERIAL GENETICS; BACTERIAL STRAIN; CROSSING OVER; ENZYME ACTIVITY; GENE DELETION; GENE INSERTION; GENE SEQUENCE; GENETIC CODE; GENOMICS; GRAM NEGATIVE BACTERIUM; MUTAGENESIS; NONHUMAN; OPERON; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; SINORHIZOBIUM MELILOTI;

ALPHAPROTEOBACTERIA; BACTERIA (MICROORGANISMS); INSERTION SEQUENCES; NEGIBACTERIA; SINORHIZOBIUM; SINORHIZOBIUM MELILOTI;

EID: 0037398045     PISSN: 03028933     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00203-003-0532-9     Document Type: Article

References (23)

1. Aneja P, Charles TC (1999) Poly-3-hydroxybutyrate degradation in Rhizobium (Sinorhizobium) meliloti: isolation and characterization of a gene encoding 3-hydroxybutyrate dehydrogenase. J Bacteriol 181:849-857
2. Ausubel FM, Brent, R, Kingston RE, Moore DD, Seidman JG, Smith JA, Struhl K (1997) Current protocols in molecular biology. Wiley, New York
3. Cai G-Q, Driscoll BT, Charles TC (2000) Requirement for the enzymes acetoacetyl coenzyme A synthetase and poly-3-hydroxybutyrate (PHB) synthase for growth of Sinorhizobium meliloti on PHB cycle intermediates. J Bacteriol 182:2113-2118
4. Charles TC, Finan TM (1991) Analysis of a 1600-kilobase Rhizobium meliloti megaplasmid using defined deletions generated in vivo. Genetics 127:5-20
5. Charles TC, Cai G-Q, Aneja P (1997) Megaplasmid and chromosomal loci for the PHB degradation pathway in Rhizobium (Sinorhizobium) meliloti. Genetics 146:1211-1220
6. Entcheva P, Phillips DA, Streit WR (2002) Functional analysis of Sinorhizobium meliloti genes involved in biotin synthesis and transport. Appl Environ Microbiol 68:2843-2848
7. Finan TM, Kunkel B, DeVos GF, Signer ER (1986) Second symbiotic megaplasmid in Rhizobium meliloti carrying exopolysaccharide and thiamine synthesis genes. J Bacteriol 167:66-72
8. 2-fixing endosymbiont Sinorhizobium meliloti. Proc Natl Acad Sci USA 98:9889-9894
9. Galibert F, Finan TM, Long SR, Pühler A, Abola P, Ampe F, Barloy-Hubler F et al (2001) The composite genome of the legume symbiont Sinorhizobium meliloti. Science 293:668-72
10. Heinz EB, Phillips DA, Streit WR (1999) BioS, a biotin-induced, stationary-phase, and possible LysR-type regulator in Sinorhizobium meliloti. Mol Plant Microbe Interact 12:803-812
11. Hofmann K, Heinz EB, Charles TC, Hoppert M, Liebl W, Streit WR (2000) Sinorhizobium meliloti strain 1021 bioS and bdhA gene transcriptions are both affected by biotin available in defined medium. FEMS Microbiol Lett182:41-44
12. Horton RM, Hunt HD, So SN (1989) Engineering hybrid genes without the use of restriction enzymes: gene splicing by overlap extension. Gene 77:61-68
13. Leimkühler S, Klipp W (1999) Role of XDHC in molybdenum cofactor insertion into xanthine dehydrogenase of Rhodobacter capsulatus. J Bacteriol 181:2745-2751
14. Leimkühler S, Kern M, Solomon PS, McEwan AG, Schwarz G, Mendel RR, Klipp W (1998) Xanthine dehydrogenase from the phototrophic purple bacterium Rhodobacter capsulatus is more similar to its eukaryotic counterparts than to prokaryotic molybdenum enzymes. Mol Microbiol 27:853-869
15. Link AJ, Phillips D, Church GM (1997) Methods for generating precise deletions and insertions in the genome of wild-type Escherichia coli: application to open reading frame characterization. J Bacteriol 179:6228-37
16. Meade HM, Long SR, Ruvkun GB, Brown SE, Ausubel FM (1982) Physical and genetic characterization of symbiotic and auxotrophic mutants of Rhizobium meliloti induced by transposon mutagenesis. J Bacteriol 149:114-122
17. Moreno-Hagelsieb G, Collado-Vides J (2002) A powerful non-homology method for the prediction of operons in prokaryotes. Bioinformatics 18:329S-336
18. Rinas U, Hellmuth K, Kang R, Seeger A, Schlieker H (1995) Entry of Escherichia coli into stationary phase is indicated by endogenous and exogenous accumulation of nucleobases. Appl Environ Microbiol 61:4147-4151
19. Schafer A, Tauch A, Jager W, Kalinowski J, Thierbach G, Pühler A (1994) Small mobilizable multi-purpose cloning vectors derived from the Escherichia coli plasmids pK18 and pK19: selection of defined deletions in the chromosome of Corynebacterium glutamicum. Gene 145:69-73
20. Streit WR, Phillips DA (1997) A biotin-regulated locus, bioS, in a possible survival operon of Rhizobium meliloti. Mol Plant Microbe Interact 10:933-937
21. Streit WR, Joseph CM, Phillips DA (1996) Biotin and other water-soluble vitamins are key growth factors for alfalfa root colonization by Rhizobium meliloti 1021. Mol Plant Microbe Interact 9:330-338
22. Tsolis R (2002) Comparative genome analysis of the α-proteobacteria: relationships between plant and animal pathogens and host specificity. Proc Natl Acad Sci USA 99:12503-12505
23. Watson RJ, Heys R, Martin T, Savard M (2001) Sinorhizobium meliloti cells require biotin and either cobalt or methionine for growth. Appl Environ Microbiol 67:3767-3770