Expression of grass carp growth hormone in the yeast Pichia pastoris

Acta Genetica Sinica

Volumn 30, Issue 4, 2003, Pages 301-306

  Wang, Wei ^a   Sun, Yong Hua ^a   Wang, Ya Ping ^a   Zhu, Zuo Yan ^a  

^a INSTITUTE OF HYDROBIOLOGY   (China)

Author keywords

ELISA; Expression; Grass carp growth hormone; Yeast pichia pastoris

Indexed keywords

GLYCERALDEHYDE 3 PHOSPHATE DEHYDROGENASE; GROWTH HORMONE; POLYCLONAL ANTIBODY;

ARTICLE; BODY GROWTH; ENZYME LINKED IMMUNOSORBENT ASSAY; GENE EXPRESSION; GENE INSERTION; GRASS; IMMUNOPRECIPITATION; MOLECULAR WEIGHT; NONHUMAN; PHYSICAL DEVELOPMENT; PICHIA PASTORIS; PROMOTER REGION; RABBIT;

ANIMALS; BINDING, COMPETITIVE; BLOTTING, WESTERN; CARPS; GENE EXPRESSION; GENETIC VECTORS; GROWTH HORMONE; PICHIA; RECEPTORS, SOMATOTROPIN; RECOMBINANT PROTEINS;

CTENOPHARYNGODON IDELLA; CYPRINUS CARPIO; INSERTION SEQUENCES; ORYCTOLAGUS CUNICULUS; PICHIA; PICHIA PASTORIS;

EID: 0037397287     PISSN: 03794172     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (10)

1. Zhu Z, He L, Chen T T. Primary-structural and evolutionary analyses of the growth-hormone gene from grass carp (ctenopharyngodon idellus). Eur J Biochem, 1992, 207:643-648.
2. Cui Z, Zhu Z, Cui Y, Li G, Xu K. Food consumption and energy budget in MThGH-transgenic F2 red carp (Cyprinus carpio L. red var.). Chinese Science Bulletin, 1996, 41:591-596.
3. Fu C, Cui Y, Hung S S O, Zhu Z. Growth and utilization by F4 human growth hormone transgenic carp fed diets with different protein levels. Journal of Fish Biology, 1998, 53:115-129.
4. WANG Wei, WANG Ya-Ping, ZHU Zuo-Yan. Prokaryotic expression of recombinant grass carp growth hormone. Acta Genetica Sinica, 2001, 28(4):306-312.
5. Tsai H J, Chen H M. Secretory synthesis of active recombinant fish growth hormone by insect cells using a baculovirus vector. Can J Fish Aquat Sci, 1994, 51:1-7.
6. Cereghino J L, Cregg J M. Heterologous protein expression in the methylotrophic yeast Pichia pastoris. FEMS Microbiology Reviews, 2000, 24:45-66.
7. Waterham H R, Digan M E, Koutz P J, Lair S V, Cregg J M. Isolation of the Pichia pastoris glyceraldehyde-3-phosphate dehydrogenase gene and regulation and use of its prometer. Gene, 1997, 186:37-44.
8. Kujan J, Herskowitz I. Structure of a yeast pheromone gene (MF-alpha): a putative alpha-factor precursor contains four tandem copies of mature alpha-factor. Cell, 1982, 30:933-943.
9. Martinez R A, Martinez P A, Lacadena J, Mancheno J M, Onaderra M, Lopez O C, Gavilanes J G. Secretion of recombinant pro- and mature fungal sarcin ribotoxion by the methylotrophic yeast Pichia pastoris: the Lys-Arg motify is required for maturation. Protein Expr Purif, 1998, 12:315-322.
10. Brierley R A. Secretion of recombinant human insulin-like growth factor I (IGF-I). Methods Mol Biol, 1998, 103: 149-177.