Combining histology and biochemical measurements of connective tissue components in small samples of lung: Application to bleomycin-induced fibrosis in the mouse

Experimental Lung Research

Volumn 29, Issue 3, 2003, Pages 179-194

  Starcher, Barry ^a   Kuhn, Charles ^b  

^a UNIVERSITY OF TEXAS HEALTH CENTER   (United States)

^b BROWN UNIVERSITY   (United States)

Author keywords

Desmosine; Elastin; Fibrosis; Lung

Indexed keywords

BLEOMYCIN; DESMOSINE; ELASTIN; PROTEIN;

ANIMAL EXPERIMENT; ANIMAL MODEL; ANIMAL TISSUE; ARTICLE; CHEMICAL ANALYSIS; CONNECTIVE TISSUE; CONTROLLED STUDY; FEMALE; HISTOLOGY; LUNG ALVEOLUS; LUNG FIBROSIS; LUNG INJURY; MEASUREMENT; MOUSE; NONHUMAN; PRIORITY JOURNAL;

ADMINISTRATION, INHALATION; ADMINISTRATION, INTRANASAL; ANIMALS; ANTIBIOTICS, ANTINEOPLASTIC; BLEOMYCIN; COLLAGEN; DESMOSINE; DOSE-RESPONSE RELATIONSHIP, DRUG; ELASTIN; FEMALE; HYDROXYPROLINE; MICE; MICE, MUTANT STRAINS; PULMONARY ALVEOLI; PULMONARY FIBROSIS; TRACHEA;

EID: 0037391597     PISSN: 01902148     EISSN: None     Source Type: Journal    
DOI: 10.1080/01902140303771     Document Type: Article

References (11)

1. Pierce JA, Hocott JB: Studies on the collagen and elastin content of the human lung. J Clin Invest. 1960;39:8-14.
2. King GS, Mohan VS, Starcher BC: Radioimmunoassay for desmosine. Connect Tissue Res. 1980;7:263-267.
3. Soskel NT: High-performance liquid chromatographic quantitation of desmosine plus isodesmosine in elastin and whole tissue hyrolysates. Anal Biochem. 1987;160:98-104.
4. Gunja-Smith Z: An enzyme-linked immunosorbent assay to quantitate the elastin crosslink desmosine in tissue and urine samples. Anal Biochem. 1985;147:258-264.
5. Chen JR, Takahashi M, Kushida K, Suzuke M, Suzuki K, Horiuchi K, Nagano A: Direct detection of crosslinks of collagen and elastin in the hydrolysates of human yellow ligament using single-column high performance liquid chromatography. Anal Biochem. 2000;278:99-105.
6. Vigilo S, Iadarola P, Lupi A, Trisolini R, Tinelli C, Balbi B, Grassi V, Worlitzsch D, Doring G, Meloni F, Meyer KC, Dowson L, Hill SL, Stockley RA, Luisetti M: MEKC of desmosine and isodesmosine in urine of chronic destructive lung disease patients. Eur Respir J. 2000;15:1039-1045.
7. D'Andrea MR, Rogahn CJ, Damiano BP, Andrade-Gordon P: A combined histochemical and double immunohistochemical labeling protocol for simultaneous evaluation of four cellular markers in restenotic arteries. Biotech Histochem. 1999;74:172-180.
8. Rakerbrandt F, Crawford DC, Havard D, Coleman D, Woodcock JP: Relationship between ultrasound texture classification images and histology of atherosclerotic plaque. Ultrasound Med Biol. 2000;26:1393-1402.
9. Starcher B, Williams I: A method for intratracheal instillation of endotoxin into the lungs of mice. Lab Anim. 1989;23:234-240.
10. Starcher B, Conrad M: Role for neutrophil elastase in the progression of solar elastosis: Connect Tissue Res. 1995;31:133-140.
11. Starcher B: A ninhydrin based assay to quantitate the total protein content of tissue samples. Anal Biochem. 2001;292:125-129.