Overexpression of the pectin lyase gene of Pseudomonas marginalis in Escherichia coli and purification of the active enzyme

Biotechnology and Applied Biochemistry

Volumn 37, Issue 2, 2003, Pages 187-194

  Papi, Rigini ^a   Kyriakidis, Dimitrios ^a  

^a ARISTOTLE UNIVERSITY OF THESSALONIKI   (Greece)

Author keywords

Affinity chromatography; Hexahistidine tagged protein; Pectin lyase inhibitor; Plasmid DNA retardation

Indexed keywords

DNA; ENZYMES; ESCHERICHIA COLI; GENES;

PROTEIN INHIBITORS;

BIOCHEMISTRY;

AGAROSE; ANTIBODY; DITHIOTHREITOL; DNA; HISTIDINE; NICKEL; NICOTINAMIDE ADENINE DINUCLEOTIDE; NITRILOTRIACETIC ACID; PECTIN LYASE; PROTEIN; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE;

AFFINITY CHROMATOGRAPHY; ARTICLE; DNA BINDING; DNA PROTEIN COMPLEX; ENZYME INHIBITION; ENZYME PURIFICATION; ESCHERICHIA COLI; GENE; GENE EXPRESSION; IMMUNOBLOTTING; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; POLYMERASE CHAIN REACTION; PSEUDOMONAS MARGINALIS;

ENZYME ACTIVATION; ESCHERICHIA COLI; GENE EXPRESSION REGULATION, BACTERIAL; GENE EXPRESSION REGULATION, ENZYMOLOGIC; POLYSACCHARIDE-LYASES; PSEUDOMONAS; RECOMBINANT PROTEINS;

ESCHERICHIA COLI; PROKARYOTA; PSEUDOMONAS; PSEUDOMONAS MARGINALIS;

EID: 0037385381     PISSN: 08854513     EISSN: None     Source Type: Journal    
DOI: 10.1042/BA20020055     Document Type: Article

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