Open-state disulfide crosslinking between Mycobacterium tuberculosis mechanosensitive channel subunits

Biophysical Journal

Volumn 84, Issue 4, 2003, Pages 2357-2365

  Shapovalov, George ^a,b   Bass, Randal ^a   Rees, Douglas C ^a   Lester, Henry A ^a  

^a CALIFORNIA INSTITUTE OF TECHNOLOGY   (United States)

^b California Institute of Technology   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

MERCAPTOETHANOL;

ARTICLE; BACTERIAL MEMBRANE; CROSS LINKING; DISULFIDE BOND; MEMBRANE CHANNEL; MEMBRANE CONDUCTANCE; MEMBRANE ELECTROPHYSIOLOGY; MUTAGENESIS; MYCOBACTERIUM TUBERCULOSIS; NONHUMAN; PATCH CLAMP; PHENOTYPE;

BACTERIA (MICROORGANISMS); MYCOBACTERIUM; MYCOBACTERIUM TUBERCULOSIS;

EID: 0037381990     PISSN: 00063495     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0006-3495(03)75041-3     Document Type: Article

References (22)

1. Benitah, J. P., G. F. Tomaselli, and E. Marban. 1996. Adjacent pore-lining residues within sodium channels identified by paired cysteine mutagenesis. Proc. Natl. Acad. Sci. USA. 93:7392-7396.
2. Betanzos, M., C. S. Chiang, H. R. Guy, and S. Sukharev. 2002. A large iris-like expansion of a mechanosensitive channel protein induced by membrane tension. Nat. Struct. Biol. 9:704-710.
3. Blount, P., S. I. Sukharev, P. C. Moe, B. Martinac, and C. Kung. 1999. Mechanosensitive channels of bacteria. Methods Enzymol. 294:458-482.
4. Butler, S. L., and J. J. Falke. 1996. Effects of protein stabilizing agents on thermal backbone motions: a disulfide trapping study. Biochemistry. 35:10595-10600.
5. Careaga, C. L., and J. J. Falke. 1992. Thermal motions of surface α-helices in the D-galactose chemosensory receptor. Detection by disulfide trapping. J. Mol. Biol. 226:1219-1235.
6. Chang, G., R. H. Spencer, A. T. Lee, M. T. Barclay, and D. C. Rees. 1998. Structure of the MscL homolog from Mycobacterium tuberculosis: a gated mechanosensitive ion channel. Science. 282:2220-2226.
7. Cruickshank, C. C., R. F. Minchin, A. C. Le Dain, and B. Martinac. 1997. Estimation of the pore size of the large-conductance mechanosensitive ion channel of Escherichia coli. Biophys. J. 73:1925-1931.
8. Elmore, D. E., and D. A. Dougherty. 2001. Molecular dynamics simulations of wild-type and mutant forms of the Mycobacterium tuberculosis MscL channel. Biophys. J. 81:1345-1359.
9. Falke, J. J., A. F. Dernburg, D. A. Sternberg, N. Zalkin, D. L. Milligan, and D. E. Koshland, Jr. 1988. Structure of a bacterial sensory receptor. A site-directed sulfhydryl study. J. Biol. Chem. 263:14850-14858.
10. Kong, Y., Y. Shen, T. E. Warth, and J. Ma. 2002. Conformational pathways in the gating of Escherichia coli mechanosensitive channel. Proc. Natl. Acad. Sci. USA. 99:5999-6004.
11. Martinac, B., M. Buechner, A. H. Delcour, J. Adler, and C. Kung. 1987. Pressure-sensitive ion channel in Escherichia coli. Proc. Natl. Acad. Sci. USA. 84:2297-2301.
12. Maurer, J. A., and D. A. Dougherty. 2001. A high-throughput screen for MscL channel activity and mutational phenotyping. Biochim. Biophys. Acta. 1514:165-169.
13. Maurer, J. A., D. E. Elmore, H. A. Lester, and D. A. Dougherty. 2000. Comparing and contrasting Escherichia coli and Mycobacterium tuberculosis mechanosensitive channels (MscL). New gain of function mutations in the loop region. J. Biol. Chem. 275:22238-22244.
14. Moe, P. C., G. Levin, and P. Blount. 2000. Correlating a protein structure with function of a bacterial mechanosensitive channel. J. Biol. Chem. 275:31121-31127.
15. Pantoliano, M. W., R. C. Ladner, P. N. Bryan, M. L. Rollence, J. F. Wood, and T. L. Poulos. 1987. Protein engineering of subtilisin BPN′: enhanced stabilization through the introduction of two cysteines to form a disulfide bond. Biochemistry. 26:2077-2082.
16. Perozo, E., D. M. Cortes, P. Sompornpisut, A. Kloda, and B. Martinac. 2002. Open channel structure of MscL and the gating mechanism of mechanosensitive channels. Nature. 418:942-948.
17. Perry, L. J., and R. Wetzel. 1986. Unpaired cysteine-54 interferes with the ability of an engineered disulfide to stabilize T4 lysozyme. Biochemistry. 25:733-739.
18. Srinivasan, N., R. Sowdhamini, C. Ramakrishnan, and P. Balaram. 1990. Conformations of disulfide bridges in proteins. Int. J. Pept. Protein Res. 36:147-155.
19. Sukharev, S. 2002. Purification of the small mechanosensitive channel of Escherichia coli (mscs): the subunit structure, conduction, and gating characteristics in liposomes. Biophys. J. 83:290-298.
20. Sukharev, S., M. Betanzos, C. S. Chiang, and H. R. Guy. 2001a. The gating mechanism of the large mechanosensitive channel MscL. Nature. 409:720-724.
21. Sukharev, S., S. R. Durell, and H. R. Guy. 2001b. Structural models of the MscL gating mechanism. Biophys. J. 81:917-936.
22. Yoshimura, K., A. Batiza, M. Schroeder, P. Blount, and C. Kung. 1999. Hydrophilicity of a single residue within MscL correlates with increased channel mechanosensitivity. Biophys. J. 77:1960-1972.