Detection of Bifidobacterium species and Gardnerella vaginalis in the vagina using PCR and denaturing gradient gel electrophoresis (DGGE)

International Journal of Gynecology and Obstetrics

Volumn 81, Issue 1, 2003, Pages 61-63

  Burton, J P ^a,b   Dixon, J L ^a,b   Reid, G ^a,b  

^a LAWSON HEALTH RESEARCH INSTITUTE   (Canada)

^b UNIVERSITY OF WESTERN ONTARIO   (Canada)

Author keywords

Bifidobacterium; DGGE; Gardnerella vaginalis; PCR

Indexed keywords

BACTERIAL DNA;

AMPLICON; ARTICLE; BACTERIAL STRAIN; BACTERIUM IDENTIFICATION; BACTERIUM ISOLATE; BIFIDOBACTERIUM; BIFIDOBACTERIUM ADOLESCENTIS; BIFIDOBACTERIUM ANIMALIS; BIFIDOBACTERIUM BIFIDUM; BIFIDOBACTERIUM BREVE; BIFIDOBACTERIUM CATENULATUM; BIFIDOBACTERIUM LONGUM; BIFIDOBACTERIUM LONGUM INFANTIS; BIFIDOBACTERIUM PSEUDOCATENULATUM; BIFIDOBACTERIUM SCARDOVI; DENATURING GRADIENT GEL ELECTROPHORESIS; DNA EXTRACTION; DNA ISOLATION; DNA SEQUENCE; ESTROGEN THERAPY; FEMALE; GARDNERELLA VAGINALIS; GENE AMPLIFICATION; HUMAN; MAJOR CLINICAL STUDY; NONHUMAN; PHENOTYPE; POLYMERASE CHAIN REACTION; POSTMENOPAUSE; PRIORITY JOURNAL; VAGINITIS;

EID: 0037380964     PISSN: 00207292     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0020-7292(02)00408-3     Document Type: Article

References (4)

1. Korshunov V.M., Gudieva Z.A., Efimov B.A., Pikina A.P., Smeianov V.V., Reid G., et al. The vaginal Bifidobacterium flora in women of reproductive age. Zh Mikrobiol Epidemiol Immunobiol. 4:1999;74-78.
2. Gavini F., Van Esbroeck M., Touzel J.P., Fourment A., Goossens H. Detection of fructose-6-phosphate phosphoketolase (F6PPK), a key enzyme of the Bifid-Shunt, in Gardnerella vaginalis. Anaerobe. 2:1996;191-193.
3. Satokari R.M., Vaughan E.E., Akkermans A.D., Saarela M., de Vos W.M. Bifidobacterial diversity in human feces detected by genus-specific PCR and denaturing gradient gel electrophoresis. Appl Environ Microbiol. 67:2001;504-513.
4. Nath K., Sarosy J.W., Stylianou S.P. Suitability of a unique 16S rRNA gene PCR product as an indicator of Gardnerella vaginalis. Biotechniques. 28:2000;222-224.