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Differentially methylated forms of histone H3 show unique association patterns with inactive human X chromosomes
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This report shows accumulation of H3-K9 methylation at the inactive X chromosome in female mammals. By contrast, methylation of H3-K4 is largely absent from this specialized heterochromatic structure. In ChIP experiments, it is shown that silenced genes on the inactive X have high levels of H3-K9 methylation, whereas the promoters of the few genes that escape the silencing process have high levels of H3-K4 methylation.
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Boggs B.A., Cheung P., Heard E., Spector D.L., Chinault A.C., Allis C.D. Differentially methylated forms of histone H3 show unique association patterns with inactive human X chromosomes. Nat. Genet. 30:2002;73-76 This report shows accumulation of H3-K9 methylation at the inactive X chromosome in female mammals. By contrast, methylation of H3-K4 is largely absent from this specialized heterochromatic structure. In ChIP experiments, it is shown that silenced genes on the inactive X have high levels of H3-K9 methylation, whereas the promoters of the few genes that escape the silencing process have high levels of H3-K4 methylation.
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Nat. Genet.
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Boggs, B.A.1
Cheung, P.2
Heard, E.3
Spector, D.L.4
Chinault, A.C.5
Allis, C.D.6
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96
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0036338205
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Histone H3 lysine 9 methylation is an epigenetic imprint of facultative heterochromatin
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Metaphase-spreads from embryonic stem cells display selective accumulation of H3-K9 methylation on the inactivated X chromosome in female mammals. In Suv39h double-null cells, methylation of H3-K9 on the inactivated X chromosome is not disrupted. The authors suggest that an unidentified H3-K9-specific HMT is responsible for the establishment of the repressive H3-K9 methylation pattern on the inactive X chromosome.
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Peters A.H., Mermoud J.E., O'Carroll D., Pagani M., Schweizer D., Brockdorff N., Jenuwein T. Histone H3 lysine 9 methylation is an epigenetic imprint of facultative heterochromatin. Nat. Genet. 30:2002;77-80 Metaphase-spreads from embryonic stem cells display selective accumulation of H3-K9 methylation on the inactivated X chromosome in female mammals. In Suv39h double-null cells, methylation of H3-K9 on the inactivated X chromosome is not disrupted. The authors suggest that an unidentified H3-K9-specific HMT is responsible for the establishment of the repressive H3-K9 methylation pattern on the inactive X chromosome.
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(2002)
Nat. Genet.
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Peters, A.H.1
Mermoud, J.E.2
O'Carroll, D.3
Pagani, M.4
Schweizer, D.5
Brockdorff, N.6
Jenuwein, T.7
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The role of chromosomal RNAs in marking the X for dosage compensation
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Kelley R.L., Kuroda M.I. The role of chromosomal RNAs in marking the X for dosage compensation. Curr. Opin. Genet. Dev. 10:2000;555-561.
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Kelley, R.L.1
Kuroda, M.I.2
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Extent of chromatin spreading determined by roX RNA recruitment of MSL proteins
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Park Y., Kelley R.L., Oh H., Kuroda M.I., Meller V.H. Extent of chromatin spreading determined by roX RNA recruitment of MSL proteins. Science. 298:2002;1620-1623.
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Science
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Park, Y.1
Kelley, R.L.2
Oh, H.3
Kuroda, M.I.4
Meller, V.H.5
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