Overexpression of the waaZ gene leads to modification of the structure of the inner core region of Escherichia coli lipopolysaccharide, truncation of the outer core, and reduction of the amount of O polysaccharide on the cell surface

Journal of Bacteriology

Volumn 185, Issue 5, 2003, Pages 1659-1671

  Frirdich, Emilisa ^a   Lindner, Buko ^b   Holst, Otto ^b   Whitfield, Chris ^a  

^a UNIVERSITY OF GUELPH   (Canada)

^b RESEARCH CENTER BORSTEL   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

ESCHERICHIA COLI LIPOPOLYSACCHARIDE;

ARTICLE; BACTERIAL GENE; BACTERIAL OUTER MEMBRANE; BACTERIAL STRAIN; CARBON NUCLEAR MAGNETIC RESONANCE; ESCHERICHIA COLI; GENE CLUSTER; GENE OVEREXPRESSION; LIPID COMPOSITION; NONHUMAN; PRIORITY JOURNAL; PROTON NUCLEAR MAGNETIC RESONANCE; STRAIN DIFFERENCE;

CARBOHYDRATE SEQUENCE; CELL MEMBRANE; ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; GENE EXPRESSION REGULATION, BACTERIAL; LIPOPOLYSACCHARIDES; MAGNETIC RESONANCE SPECTROSCOPY; MOLECULAR SEQUENCE DATA; MULTIGENE FAMILY; MUTATION; O ANTIGENS; SPECTROMETRY, MASS, MATRIX-ASSISTED LASER DESORPTION-IONIZATION; SUGAR ACIDS;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; NEGIBACTERIA; SALMONELLA; SALMONELLA ENTERICA;

EID: 0037371328     PISSN: 00219193     EISSN: None     Source Type: Journal    
DOI: 10.1128/JB.185.5.1659-1671.2003     Document Type: Article

References (47)

1. Altschul, S. F., W. Gish, W. Miller, E. W. Myers, and D. J. Lipman. 1990. Basic local alignment search tool. J. Mol. Biol. 215:403-410.
2. Altschul, S. F., T. L. Madden, A. A. Schaffer, J. Zhang, Z. Zhang, W. Miller, and D. J. Lipman. 1997. Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res. 25:3389-3402.
3. Amor, K., D. E. Heinrichs, E. Frirdich, K. Ziebell, R. P. Johnson, and C. Whitfield. 2000. Distribution of core oligosaccharide types in lipopolysaccharides from Escherichia coli. Infect. Immun. 68:1116-1124.
4. Amor, P. A., and C. Whitfield. 1997. Molecular and functional analysis of genes required for expression of group IB K antigens in Escherichia coli: characterization of the his-region containing gene clusters for multiple cell-surface polysaccharides. Mol. Microbiol. 26:145-161.
5. Appelmelk, B. J., Y.-Q. An, T. A. M. Hekker, L. G. Thijs, D. M. MacLaren, and J. de Graaf. 1994. Frequencies of lipopolysaccharide core types in Escherichia coli strains from bacteraemic patients. Microbiology 140:1119-1124.
6. Binotto, J., P. R. MacLachlan, and P. R. Sanderson. 1991. Electrotransformation of Salmonella typhimurium LT2. Can. J. Microbiol. 37:474-477.
7. Brabetz, W., S. Miiller-Loennies, O. Holst, and H. Brade. 1997. Deletion of the heptosyltransferase genes rfaC and rfaF in Escherichia coli K-12 results in an Re-type lipopolysaccharide with a high degree of 2-aminoethanol phosphate substitution. Eur. J. Biochem. 247:716-724.
8. Campbell, J. A., G. J. Davies, V. Bulone, and B. Henrissat. 1997. A classification of nucleotide-diphospho-sugar glycosyltransferases based on amino acid sequence similarities. Biochem. J. 326:929-939.
9. Coutinho, P. M., and B. Henrissat. 1999. Carbohydrate-active enzymes: an integrated database approach, p. 3-12. In G. D. H. J. Gilbert, B. Henrissat, and B. Svensson (ed), Recent advances in carbohydrate bioengineering. The Royal Society of Chemistry, Cambridge, United Kingdom.
10. Coutinho, P. M., and B. Henrissat. 1999. The modular structure of cellulases and other carbohydrate-active enzymes: an integrated database approach, p. 15-23. In K. H. K. Ohmiya, K. Sakka, Y. Kobayashi, S. Karita, and T. Kimura (ed.), Genetics, biochemistry and ecology of cellulose degradation. Uni Publishers Co., Tokyo, Japan.
11. Currie, C. G., and I. R. Poxton. 1999. The lipopolysaccharide core type of Escherichia coli O157:H7 and other non-O157 verotoxin-producing E. coli. FEMS Immunol. Med. Microbiol. 24:57-62.
12. Finan, T. M., S. Weidner, K. Wong, J. Buhrmester, P. Chain, F. J. Vorholter, I. Hernandez-Lucas, A. Becker, A. Cowie, J. Gouzy, B. Golding, and A. Puhler. 2001. The complete sequence of the 1,683-kb pSymB megaplasmid from the N2-fixing endosymbiont Sinorhizobium meliloti. Proc. Natl. Acad. Sci. USA 98:9889-9894.
13. Galanos, C., O. Luderitz, and O. Westphal. 1969. A new method for the extraction of R lipopolysaccharides. Eur. J. Biochem. 9:245-249.
14. Gronow, S., W. Brabetz, and H. Brade. 2000. Comparative functional characterization in vitro of heptosyltransferase I (WaaC).and II and II (WaaF) from Escherichia coli. Eur. J. Biochem. 267:6602-6611.
15. BAD promoter. J. Bacteriol. 177:4121-4130.
16. Hamilton, C. A., M. Aldea, B. K. Washburn, P. Babtizke, and S. R. Kushner. 1989. New method of generating deletions and gene replacements in Escherichia coli. J. Bacteriol. 171:4617-4622.
17. Hämmerling, G., O. Lnderitz, O. Westphal, and P. H. Mäkelä. 1971. Structural investigations on the core polysaccharide of Escherichia coli O100. Eur. J. Biochem. 22:331-344.
18. Heinrichs, D. E., M. A. Monteiro, M. B. Perry, and C. Whitfield. 1998. The assembly system for the lipopolysaccharide R2 core-type Escherichia coli is a hybrid of those found in Escherichia coli K-12 and Salmonella enterica. J. Biol. Chem. 273:8849-8859.
19. Heinrichs, D. E., J. A. Yethon, P. A. Amor, and C. Whitfield. 1998. The assembly system for the outer core portion of R1- and R4-type lipopolysaccharides of Escherichia coli. J. Biol. Chem. 273:29497-29505.
20. Heinrichs, D. E., J. A. Yethon, and C. Whitfield. 1998, Molecular basis for structural diversity in the core regions of the lipopolysaccharides of Escherichia coli and Salmonella enterica. Mol. Microbiol. 30:221-232.
21. Hitchcock, P. J., and T. M. Brown. 1983. Morphological heterogeneity among Salmonella lipopolysaccharide chemotypes in silver-stained polyacrylamide gels. J. Bacteriol. 154:269-277.
22. Holst, O. 1999. Chemical structure of the core region of lipopolysaccharides, p. 115-154. In H. Brade, S. M. Opal, S. N. Vogel, and D. C. Morrison (ed.), Endotoxin in health and disease. Marcel Dekker, Inc., New York, N.Y.
23. Holst, O. 2000. Deacylation of lipopolysaccharides and isolation of oligosacharide phosphates, p. 345-353. In O. Holst (ed.), Methods in molecular biology, bacterial toxins: methods and protocols, vol. 145. Humana Press, Inc., Totowa, N.J.
24. Holst, O., K. Bock, L. Brade, and H. Brade. 1995. The structures of oligosaccharide bisphosphates isolated from the lipopolysaccharide of a recombinant Escherichia coli strain expressing the gene gseA [3-deoxy-D-manno-octulopyranosonic acid (Kdo) transferase] of Chlamydia psittaci 6BC. Eur. J. Biochem. 229:194-200.
25. Holst, O., and H. Brade. 1992. Chemical structure of the core region of lipopolysaccharides, p. 134-170. In D. C. Morrison and J. L. Ryan (ed.), Bacterial endotoxic lipopolysaccharides, vol. I. CRC Press, Boca Raton, Fla.
26. Holst, O., U. Zähringer, H. Brade, and A. Zamojski. 1991. Structural analysis of the heptose/hexose region of the lipopolysaccharide from Eseherichia coli K-12 strain W3100. Carbohydr. Res. 215:323-335.
27. Hull, R. A., R. E. Gill, P. Hsu, B. H. Minshew, and S. Falkow. 1981. Construction and expression of recombinant plasmids encoding type I or mannose-resistant pili from a urinary tract infection Escherichia coli isolate. Infect. Immun. 33:933-938.
28. Kaniuk, N. A., M. A. Monteiro, C. T. Parker, and C. Whitfield. 2002. Molecular diversity of the genetic loci responsible for lipopolysaccharide core oligosaccharide assembly within the genus Salmonella. Mol. Microbiol. 46:1305-1318.
29. Knirel, Y. A., O. V. Bystrova, A. S. Shashkov, B. Lindner, N. A. Kocharova, S. N. Senchenkova, H. Moll, U. Zahringer, K. Hatano, and G. B. Pier. 2001. Structural analysis of the lipopolysaccharide core of a rough, cystic fibrosis isolate of Pseudomonas aeruginosa. Eur. J. Biochem. 268:4708-4719.
30. Lindner, B. 2000. Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry of lipopolysaccharides, p. 311-325. In O. Holst (ed.), Methods in molecular biology, bacterial toxins: methods and protocols, vol. 145. Humana Press, Inc., Totowa, N.J.
31. Müller-Loennies, S., O. Holst, B. Lindner, and H. Brade. 1999. Isolation and structural analysis of phosphorylated oligosaccharides obtained from Escherichia coli J-5 lipopolysaccharide. Eur. J. Biochem, 260:235-249.
32. Raetz, C. R. H., and C. Whitfield. 2002. Lipopolysaccharide endotoxins. Annu. Rev. Biochem. 71:635-700.
33. Regué, M., N. Climent, N. Abitiu, N. Coderch, S. Merino, L. Izquierdo, M. Altarriba, and J. M. Tomás. 2001. Genetic characterization of the Klebsiella pneumoniae waa gene cluster, involved in core lipopolysaccharide biosynthesis. J. Bacteriol. 183:3564-3573.
34. Sambrook, J., E. F. Fritsch, and T. Maniatis. 1989. Molecular cloning: a laboratory manual, 2nd ed. Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y.
35. Schmidt, G., I. Fromme, and H. Mayer. 1970. Immunochemical studies on core lipopolysaccharides of Enterobacteriaceae of different genera. Eur. J. Biochem. 14:357-366.
36. -. Eur. J. Biochem. 42:303-309.
37. Schmidt, G., B. Jann, and K. Jann. 1969. Immunochemistry of R lipopolysaccharides of Escherichia coli. Different core regions in the lipopolysaccharides of O group 8. Eur. J. Biochem. 10:501-510.
38. Schnaitman, C. A., and J. D. Klena. 1993. Genetics of lipopolysaccharide biosynthesis in enteric bacteria. Microbiol. Rev. 57:655-682.
39. Sugai, T., C. H. Lin, G. J. Shen, and C. H. Wong. 1995. CMP-KDO synthetase: overproduction and application to the synthesis of CMP-KDO and analogs. Bioorg. Med. Chem. 3:313-320.
40. -). J. Biol. Chem. 273:7006-7017.
41. Tsai, G. M., and C. E. Frasch. 1982. A sensitive silver stain for detecting lipopolysaccharides in polyacrylamide gels. Anal. Biochem. 119:115-119.
42. Vinogradov, E., M. Cedzynski, A. Ziolkowski, and A. Swierzko. 2001. The structure of the core region of the lipopolysaccharide from Klebsiella pneumoniae O3. Eur. J. Biochem. 268:1722-1729.
43. Vinogradov, E., and M. B. Perry. 2001. Structural analysis of the core region of the lipopolysaccharides from eight serotypes of Klebsiella pneumoniae. Carbohydr. Res. 335:291-296.
44. Vinogradov, E. V., K. Van Der Drift, J. E. Thomas-Oates, S. Meshkov, H. Brade, and O. Holst. 1999. The structures of the carbohydrate backbones of the lipopolysaccharides from Escherichia coli rough mutants F470 (R1 core type) and F576 (R2 core type). Eur. J. Biochem. 261:629-639.
45. Whitfield, C., G. Schoenhals, and L. Graham. 1989. Mutants of Escherichia coli 09:K30 with altered synthesis and expression of the capsular K antigen. J. Gen. Microbiol. 135:2589-2599.
46. Yethon, J. A., J. S. Gunn, R. K. Ernst, S. I. Miller, L. Laroche, D. Malo, and C. Whitfield. 2000. Salmonella enterica serovar Typhimurium waaP mutants show increased susceptibility to polymyxin and loss of virulence in vivo. Infect. Immun. 68:4485-4491.
47. Yethon, J. A., D. E. Heinrichs, M. A. Monteiro, M. B. Perry, and C. Whitfield. 1998. Involvement of waaY, waaQ, and waaP in the modification of Escherichia coli lipopolysaccharide and their role in the formation of a stable outer membrane. J. Biol. Chem. 273:26310-26316.