Calcaelin, a new protein with translation-inhibiting, antiproliferative and antimitogenic activities from the mosaic puffball mushroom Calvatia caelata

Planta Medica

Volumn 69, Issue 3, 2003, Pages 212-217

  Ng, Tzi Bun ^a   Lam, Ying Wai ^a   Wang, Hexiang ^b  

^a and Hong Kong Branch of CAS Center for Excellence in Animal Evolution and Genetics   (Hong Kong)

^b CHINA AGRICULTURAL UNIVERSITY   (China)

Author keywords

Calvatia caelata; Lycoperdaceae; Lycoperdales; Mushroom; Ribosome inactivating protein

Indexed keywords

ALPHA SARCIN; CALCAELIN; CALVATIA CAELATA EXTRACT; HYDROCHLORIC ACID; PLANT EXTRACT; RESTRICTOCIN; RIBOSOME INACTIVATING PROTEIN; UNCLASSIFIED DRUG;

ADSORPTION; AMINO ACID SEQUENCE; ANIMAL CELL; ANTIBACTERIAL ACTIVITY; ANTIFUNGAL ACTIVITY; ARTICLE; BREAST CANCER; CALVATIA CAELATA; CELL PROLIFERATION; CHROMATOGRAPHY; CONTROLLED STUDY; DRUG ACTIVITY; DRUG ISOLATION; DRUG PURIFICATION; DRUG SYNTHESIS; ELUTION; FUNGUS; HEMAGGLUTINATION; MITOGENICITY; MOLECULAR WEIGHT; MOUSE; MUSHROOM; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; RETICULOCYTE; SEQUENCE HOMOLOGY; SPLEEN CELL; TRANSLATION REGULATION;

ANIMALS; ANTI-BACTERIAL AGENTS; ANTI-INFECTIVE AGENTS; ANTINEOPLASTIC AGENTS, PHYTOGENIC; BACTERIA; BASIDIOMYCOTA; CELL DIVISION; ELECTROPHORESIS, POLYACRYLAMIDE GEL; HUMANS; MICE; MICE, INBRED C57BL; MICROBIAL SENSITIVITY TESTS; PHYTOTHERAPY; PLANT EXTRACTS; PLANT PROTEINS; RABBITS; RIBONUCLEASES; SPLEEN; TUMOR CELLS, CULTURED;

ANIMALIA; BASIDIOMYCOTA; CALVATIA; FUNGI; LYCOPERDACEAE; LYCOPERDALES; ORYCTOLAGUS CUNICULUS;

EID: 0037362020     PISSN: 00320943     EISSN: None     Source Type: Journal    
DOI: 10.1055/s-2003-38492     Document Type: Article

References (24)

1. Barbieri L, Battelli MG, Stirpe F. Ribosome-inactivating proteins from plants. Biochim Biophys Acta 1993; 1154: 237-82
2. Fernandez-Luna JL, Lopez-Otin C, Soriano F, Mendez E. Complete amino acid sequence of the Aspergillus cytotoxin mitogillin. Biochemistry 1985; 24: 861-7
3. Lamy B, Davies J. Isolation and nucleotide sequence of the Aspergillus restrictocin gene coding for the ribonucleolytic toxin restrictocin and its expression in Aspergillus nidulans: the leader sequence protects producing strains from suicide. Nucleic Acids Res 1991; 19: 1001-6
4. Turnay J, Olmo N, Jiminez A, Lizarbe M, Gavilanes J. Kinetic study of the cytotoxic effect of α-sarcin, a ribosome inactivating protein from Aspergillus giganteus, on tumor cell line: protein biosynthesis inhibition and cell binding. Mol Cell Biochem 1993; 122: 39-47
5. Laemmli UK, Favre M. Maturation of the head of bacteriophage II. J Mol Biol 1973; 80: 575-99
6. Lam SSL, Wang HX, Ng TB. Purification and characterization of novel ribosome inactivating proteins, alpha- and beta-pisavins, from seeds of the garden pea Pisum sativum. Biochem Biophys Res Commun 1998; 253: 135-42
7. Mock JWY, Ng TB, Wong RNS, Yao QZ, Yeung HW, Fong WP. Demonstration of ribonuclease activity in the plant ribosome-inactivating proteins alpha- and beta-momorcharins. Life Sciences 1996; 59: 1853-9
8. Wang HX, Ng TB, Ooi VEC. A ribonuclease from sclerotia of the edible mushroom Pleurotus tuber-regium. Biochem Biophys Res Commun 1998; 250: 544-6
9. Endo Y, Tsurugi K. N-Glycosidase activity of ricin A chain. Mechanism of action of toxic lectin rich on eukaryotic ribosomes. J Biol Chem 1987; 262: 8128-30
10. Wang HX, Ng TB. Studies on the anti-mitogenic, anti-phage and hypotensive effects of several ribosome inactivating proteins. Comp Biochem Physicol Part C 2001; 128: 359-66
11. Tsao SW, Ng TB, Yeung HW. Toxicities of trichosanthin and alpha-momorcharin, abortifacient proteins from Chinese medicinal plants, on cultured tumor cell lines. Toxicon 1990; 28: 1183-92
12. Ooi LSM, Ng TB, Geng Y, Sun SSM, Ooi VEC. Lectins from bulbs of the Chinese daffodil Narcissus tazetta (family Amaryllidaceae). Biochem Cell Biol 2000; 78: 463-8
13. Lam YW, Wang HX, Ng TB. A robust cysteine-deficient chitinase-like antifungal protein from inner shoots of the edible chive Allium tuberosum. Biochem Biophys Res Commun 2000; 279: 74-80
14. Wang HX, Ng TB, Liu WK, Ooi VEC, Chang ST. Isolation and characterization of two distinct lectins with antiproliferative activity from the mycelium of the edible mushroom Tricholoma mongolicum. Int J Peptide Protein Res 1995; 46: 508-13
15. Wong RNS, Dong TX, Ng TB, Choi WT, Yeung HW. Alpha-kirilowin, a novel ribosome-inactivating protein from seeds of Trichosanthes kirilowii (Family Cucurbitaceae): a comparison with β-kirilowin and other related proteins. Int J Peptide Protein Res 1996; 47: 103-9
16. Wool IG. In: Ribonucleases. Structures and Functions, Allessio GD, Riordan JF, editors. Academic Press, New York: 1997: 131-62
17. Wang HX, Ng TB. Quinqueginsin, a novel protein with anti-human immunodeficiency virus, antifungal, ribonuclease and cell-free translation-inhibitory activities from American ginseng roots. Biochem 8iophys Res Commun 2000; 269: 203-8
18. Endo Y, Wool IG. The site of action of α-sarcin on eukaryotic ribosomes. The sequence at the α-sarcin cleavage site in 28S ribosomal ribonucleic acid. Biochem Biophys Res Commun 1982; 257: 9054-60
19. Gao W, Ling J, Zhong X, Liu W, Zhang R, Yang H, Gao H, Zhang Z. Luffin S - a small novel ribosome-inactivating protein from Luffa cylindrical. FEBS Lett 1994; 347: 257-60
20. Endo Y, Huber PW, Wool IG. The ribonuclease activity of the cytotoxin α-sarcin. The characteristics of the enzymatic activity of α-sarcin with ribosomes and ribonucleic acid as substrates. J Biol Chem 1983; 258: 2662-7
21. Roberts WK, Selitrennikoff CP. Isolation and partial characterization of two antifungal proteins from barley. Biochim Biophys Acta 1986; 880: 161-70
22. Zhu RH, Ng TB, Yeung HW, Shaw PC. High level synthesis of biologically active recombinant trichosanthin in Escherichia coli. Int J Peptide Protein Res 1991; 39: 77-81
23. Lacadena J, Pozo AM, Barbero J, Mancheno JM, Gasset M, Onaderra M, Lopez-Otin C, Ortega S, Garcia J, Gavilanes JG. Overproduction and purification of biologically active native fungal α-sarcin in Escherichia coli. Gene 1994; 142: 147-51
24. Chan WY, Ng TB, Yeung HW. Differential abilities of the ribosome inactivating proteins luffaculin, luffins and momorcochin to induce abnormalities in developing mouse embryos in vitro. Gen Comp Endocrinol 1994; 25: 363-70