Large-scale discovery of induced point mutations with high-throughput TILLING

Genome Research

Volumn 13, Issue 3, 2003, Pages 524-530

  Till, Bradley J ^a   Reynolds, Steven H ^a   Greene, Elizabeth A ^a   Codomo, Christine A ^a   Enns, Linda C ^b   Johnson, Jessica E ^b   Burtler, Chris ^b   Odden, Anthony R ^a   Young, Kim ^a   Taylor, Nicholas E ^a   Henikoff, Jorja G ^a   Comai, Luca ^b   Henikoff, Steven ^a  

^a USA   (United States)

^b UNIVERSITY OF WASHINGTON   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ADENOSINE TRIPHOSPHATE; MESYLIC ACID ETHYL ESTER;

ALLELE; ANALYTIC METHOD; ARABIDOPSIS; ARTICLE; CHEMICAL MUTAGENESIS; GENE LOCUS; GENE SEQUENCE; GENE TARGETING; GENETIC SCREENING; MEDICAL RESEARCH; MUTATION RATE; NONHUMAN; POINT MUTATION; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL;

ALKYLATING AGENTS; ARABIDOPSIS; DNA MUTATIONAL ANALYSIS; DNA, PLANT; ETHYLNITROSOUREA; GENES, PLANT; GENOME, PLANT; INTERNET; MUTAGENESIS; POINT MUTATION; SOFTWARE;

ARABIDOPSIS;

EID: 0037352032     PISSN: 10889051     EISSN: None     Source Type: Journal    
DOI: 10.1101/gr.977903     Document Type: Article

References (19)

1. Altschul, S.F., Gish, W., Miller, W., Myers, E.W., and Lipman, D.J. 1990. Basic local alignment search tool. J. Mol. Biol. 215: 403-410.
2. Ashburner, M. 1989. Drosophila. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
3. Colbert, T., Till, B.J., Tompa, R., Reynolds, S., Steine, M.N., Yeung, A.T., McCallum, C.M., Comai, L., and Henikoff, S. 2001. High-throughput screening for induced point mutations. Plant Physiol. 126: 480-484.
4. Fraser, A.G., Kamath, R.S., Zipperlen, P., Martinez-Campos, M., Sohrmann, M., and Ahringer, J. 2000. Functional genomic analysis of C. elegans chromosome I by systematic RNA interference. Nature 408: 325-330.
5. Giaever, G., Chu, A.M., Ni, L., Connelly, C., Riles, L., Veronneau, S., Dow, S., Lucau-Danila, A., Anderson, K., Andre, B., et al. 2002. Functional profiling of the Saccharomyces cerevisiae genome. Nature 418: 387-391.
6. Henikoff, J.G., Greene, E.A., Pietrokovski, S., and Henikoff, S. 2000. Increased coverage of protein families with the blocks database servers. Nucleic Acids Res. 28: 228-230.
7. Liu, L.X., Spoerke, J.M., Mulligan, E.L. Chen, J., Reardon, B., Westlund, B., Sun, L., Abel, K., Armstrong, B., Hardiman, G., et al. 1999. High-throughput isolation of Caenorhabditis elegans deletion mutants. Genome Res. 9: 859-867.
8. Markiewicz, P., Kleina, L.G., Cruz, C., Ehret, S., and Miller, J.H. 1994. Genetic studies of the lac repressor. XIV. Analysis of 4000 altered Escherichia coli lac repressors reveals essential and non-essential residues, as well as "spacers" which do not require a specific sequence. J. Mol. Biol. 240: 421-433.
9. McCallum, C.M., Comai, L., Greene, E.A., and Henikoff, S. 2000a. Targeted screening for induced mutations. Nat. Biotechnol. 18: 455-457.
10. McCallum, C.M., Comai, L., Greene, E.A., and Henikoff, S. 2000b. Targeting induced local lesions in genomes (TILLING) for plant functional genomics. Plant Physiol. 123: 439-442.
11. Ng, P.C. and Henikoff, S. 2001. Predicting deleterious amino acid substitutions. Genome Res. 11: 863-874.
12. Piano, F., Schetter, A.J., Mangone, M., Stein, L., and Kemphues, K.J. 2000. RNAi analysis of genes expressed in the ovary of Caenorhabditis elegans. Curr. Biol. 10: 1619-1622.
13. Ross-Macdonald, P., Coelho, P.S., Roemer, T., Agarwal, S., Kumar, A., Jansen, R., Cheung, K.H., Sheehan, A., Symoniatis, D., Umansky, L., et al. 1999. Large-scale analysis of the yeast genome by transposon tagging and gene disruption. Nature 402: 413-418
14. Rozen, S. and Skaletsky, H. 2000. Primer3 on the WWW for general users and for biologist programmers. Methods Mol. Biol. 132: 365-386.
15. Till, B., Colbert, T., Tompa, R., Enns, L., Codomo, C., Johnson, J., Reynolds, S., Henikoff, J., Greene, E., Steine, M., et al. 2003. High-throughput TILLING for functional genomics. In Plant functional genomics: Methods and protocols (ed. E. Grotewald). Humana Press, Totowa, NJ (in press).
16. Tompa, R., McCallum, C.M., Delrow, J., Henikoff, J.G., van Steensel, B., Henikoff, S. 2002. Genome-wide profiling of DNA methylation reveals transposon targets of CHROMOMETHYLASE3 Curr. Biol. 12:65-68.
17. Torii, K.U., Mitsukawa, N., Oosumi, T., Matsuura, Y., Yokoyama, R., Whittier, R.F., and Komeda, Y. 1996. The Arabidopsis ERECTA gene encodes a putative receptor protein kinase with extracellular leucine-rich repeats. Plant Cell 8: 735-746.
18. Winzeler, E.A., Shoemaker, D.D., Astromoff, A., Liang, H., Anderson, K., Andre, B., Bangham, R., Benito, R., Boeke, J.D., Bussey, H., et al. 1999. Functional characterization of the S. cerevisiae genome by gene deletion and parallel analysis. Science 285: 901-906.
19. Zambrowicz, B.P., Friedrich, G.A., Buxton, E.C., Lilleberg, S.L., Person, C., and Sands, A.T. 1998. Disruption and sequence identification of 2,000 genes in mouse embryonic stem cells. Nature 392: 608-611.