Efficient gene delivery into murine ovarian cells by intraovarian injection of plasmid DNA and subsequent in vivo electroporation

Genesis (United States)

Volumn 35, Issue 3, 2003, Pages 169-174

  Sato, Masahiro ^a   Tanigawa, Maya ^b   Kikuchi, Natsuko ^b   Nakamura, Shingo ^b   Kimura, Minoru ^b  

^a TOKAI UNIVERSITY   (Japan)

^b TOKAI UNIVERSITY SCHOOL OF MEDICINE   (Japan)

Author keywords

Electroporation; Gene transfer; Green fluorescent protein; LacZ; Ovary; Transfection

Indexed keywords

PLASMID DNA; TRYPAN BLUE;

ANIMAL CELL; ANIMAL EXPERIMENT; ARTICLE; DISPERSION; DNA TRANSFECTION; ELECTROPORATION; FEMALE; GENE ACTIVITY; GENE DELIVERY SYSTEM; GENE EXPRESSION; GENE TRANSFER; IN VIVO STUDY; MICROPIPETTE; MOUSE; NONHUMAN; OOCYTE; OVARY; OVARY FOLLICLE CELL; PRIORITY JOURNAL; REPORTER GENE;

MURINAE;

EID: 0037351355     PISSN: 1526954X     EISSN: None     Source Type: Journal    
DOI: 10.1002/gene.10182     Document Type: Article

References (7)

1. Gordon JW. 2001. Direct exposure of mouse ovaries and oocytes to high doses of an adenovirus gene therapy vector fails to lead to germ cell transduction. Mol Ther 3:557-564.
2. Muramatsu T, Nakamura A, Park H-M. 1998. In vivo electroporation: a powerful and convenient means of nonviral gene transfer to tissues of living animals. Int J Mol Med 1:55-62.
3. Niwa H, Yamamura K, Miyazaki J. 1991. Efficient selection for high-expression transfectants with a novel eukaryotic vector. Gene 108:193-200.
4. Sato M, Ishikawa A, Kimura M. 2002. Direct injection of foreign DNA into mouse testis as a possible in vivo gene transfer system via epididymal spermatozoa. Mol Reprod Dev 61:49-56.
5. Tsukui T, Miyake S, Azuma S, Ichise H, Saito I, Toyoda Y. 1995. Gene transfer and expression in mouse preimplantation embryos by recombinant adenovirus vector. Mol Reprod Dev 42:291-297.
6. Yamazaki Y, Fujimoto H, Ando H, Ohyama T, Hirota Y, Noce T. 1998. In vivo gene transfer to mouse spermatogenic cells by deoxyribonucleic acid injection into seminiferous tubules and subsequent electroporation. Biol Reprod 59:1439-1444.
7. Yamazaki Y, Yagi T, Ozaki T, Imoto K. 2000. In vivo gene transfer to mouse spermatogenic cells using green fluorescent protein as a marker. J Exp Zool 286:212-218.