Comparison of quantification of histochemical staining by hue-saturation-intensity (HSI) transformation and color-deconvolution

Applied Immunohistochemistry and Molecular Morphology

Volumn 11, Issue 1, 2003, Pages 85-91

  Ruifrok, Arnout C ^a,b   Katz, Ruth L ^a   Johnston, Dennis A ^a  

^a UNIVERSITY OF TEXAS   (United States)

^b BAYLOR COLLEGE OF MEDICINE   (United States)

Author keywords

Color deconvolution; Color separation; Computer assisted; Image processing; Immunohistochemistry

Indexed keywords

KI 67 ANTIGEN;

ANTIGEN EXPRESSION; ARTICLE; CELL PROLIFERATION; COLOR; COMPUTER ASSISTED DIAGNOSIS; CONTROLLED STUDY; HISTOCHEMISTRY; HUMAN; HUMAN TISSUE; IMAGE ANALYSIS; IMAGE PROCESSING; IMMUNOHISTOCHEMISTRY; LUNG PARENCHYMA; METHODOLOGY; PRIORITY JOURNAL; STANDARD;

EID: 0037347757     PISSN: 15412016     EISSN: None     Source Type: Journal    
DOI: 10.1097/00129039-200303000-00014     Document Type: Article

References (9)

1. Lehr H-A, Mankoff DA, Corwin D, Santeusanio G, Gown AM. Application of Photoshop-based image analysis to quantification of hormone receptor expression in breast cancer. J Histochem Cytochem 1997;11:1559-1565.
2. Lehr H-A, van der Loos CM, Teeling P, Gown AM. Complete chromogen separation and analysis in double immunohistochemical stains using Photoshop-based image analysis. I 1999;47:119-125.
3. Kai M, Nunobiki O, Taniguchi E, Sakamoto Y, Kakudo K. Quantitative and qualitative analysis of stain color using RGB computer color specification. Anal Quant Cytol Histol 1999;21:477-480.
4. Ruifrok AC. Quantification of immunohistochemical staining by color translation and automated thresholding. Anal Quant Cytol Histol 1997;19:107-113.
5. Montorini R, Diamanti L, Thompson D, Bartels HG, Bartels PH. Analysis of the capillary architecture in the precursors of prostate cancer: recent findings and new concepts. Eur Urol 1996;30:191-200.
6. Ruifrok AC, Johnston DA. Quantification if histochemical staining by color deconvolution. Analyt Quant Cytol Histol 2001;23:291-299.
7. Pinder SE, Wenyck P, Sibbering DM, et al. Assessment of the new proliferation marker MIB1 in breast carcinoma using image analysis: associations with other prognostic factors and survival. Br J Cancer 1995;71:146-149.
8. nd edition, Addison-Wesley, New York 1992.
9. th edition. Prentice-Hall, Upper Saddle River, New Jersey 1999.