Stability of the prion protein-encoding (PRNP) gene in HeLa cells

Biologicals

Volumn 31, Issue 1, 2003, Pages 83-86

  Amexis, Georgios ^a   Ridge, Jeanette ^a   Cervenakova, Larisa ^b   Enterline, Joan C ^a   Chumakov, Konstantin M ^a   Asher, David M ^a  

^a CENTER FOR DRUG EVALUATION AND RESEARCH   (United States)

^b HOLLAND LABORATORY   (United States)

Author keywords

Creutzfeldt Jakob disease; Gene stability; HeLa cell; Prion; PRNP; PrP protein; Transmissible spongiform encephalopathies

Indexed keywords

COMPLEMENTARY DNA; PRION PROTEIN; RNA;

ARTICLE; CELL CULTURE; CELL LINE; CELL LINEAGE; CHROMOSOME LOSS; CONTROLLED STUDY; GENE DELETION; GENE MUTATION; GENETIC STABILITY; HELA CELL; HETEROPLOIDY; HOMOZYGOSITY; HUMAN; HUMAN CELL; PRION DISEASE; PRIORITY JOURNAL; PROTEIN DEFECT; PROTEIN EXPRESSION; RISK FACTOR; TIME;

EID: 0037335417     PISSN: 10451056     EISSN: None     Source Type: Journal    
DOI: 10.1016/S1045-1056(02)00069-6     Document Type: Article

References (6)

1. Cashman C.R. Transmissible spongiform encephalopathies: vaccine issues. Dev Biol. 106:2001;455-461.
2. Gartler S.M. Apparent HeLa cell contamination of human heteroploid cell lines. Nature. 217:(5130):1968;750-751.
3. Gey G.O., Coffman W.D., Kubicek M.T. Tissue culture studies of the proliferative capacity of cervical carcinoma and normal epithelium. Cancer Res. 12:1952;264-265.
4. Krause P.R., Lewis A.M. Jr. Safety of viral DNA in biological products. Biologicals. 26:(4):1998;317-320.
5. Prusiner S.B. Shattuck lecture - neurodegenerative diseases and prions. N Engl J Med. 344:(20):2001;1516-1526.
6. Vnencak-Jones C.L., Phillips J.A. Identification of heterogeneous PRNP gene deletions in controls by detection of allele-specific heteroduplexes. Am J Hum Genet. 50:(4):1992;871-872.