Microscopic examination of intracellular organisms in protected bronchoalveolar mini-lavage fluid for the diagnosis of ventilator-associated pneumonia

Chest

Volumn 123, Issue 2, 2003, Pages 518-523

  Sirvent, Josep Maria ^a   Vidaur, Loreto ^a   Gonzalez, Susana ^a   Castro, Pilar ^a   De Batlle, Jordi ^a   Castro, Antoni ^a   Bonet, Alfons ^a  

^a HOSPITAL JOSEP TRUETA   (Spain)

Author keywords

Intracellular organisms; Microscopic examination; Previous antibiotic; Protected bronchoalveolar mini lavage; Ventilator associated pneumonia

Indexed keywords

ANTIBIOTIC AGENT;

ADULT; AGED; ANTIBIOTIC THERAPY; AREA UNDER THE CURVE; ARTICLE; ARTIFICIAL VENTILATION; BACTERIAL GROWTH; BACTERIUM CULTURE; BRONCHOSCOPY; CENTRIFUGATION; CLINICAL TRIAL; COMPARATIVE STUDY; CONFIDENCE INTERVAL; CONTROLLED CLINICAL TRIAL; CONTROLLED STUDY; CYTOLOGY; DIAGNOSTIC ACCURACY; DIAGNOSTIC PROCEDURE; DIAGNOSTIC VALUE; FEMALE; GIEMSA STAIN; GRAM STAINING; HUMAN; INTENSIVE CARE UNIT; LOWER RESPIRATORY TRACT; LUNG LAVAGE; MAJOR CLINICAL STUDY; MALE; MICROBIOLOGICAL EXAMINATION; MICROSCOPY; PNEUMONIA; PRIORITY JOURNAL; QUANTITATIVE ANALYSIS; RECEIVER OPERATING CHARACTERISTIC; SENSITIVITY AND SPECIFICITY; STATISTICAL SIGNIFICANCE; UNIVERSITY HOSPITAL; VENTILATOR;

EID: 0037331521     PISSN: 00123692     EISSN: None     Source Type: Journal    
DOI: 10.1378/chest.123.2.518     Document Type: Article

References (29)

1. Torres A, Aznar R, Gatell JM, et al. Incidence, risk and prognosis of nosocomial pneumonia in mechanically ventilated patients. Am Rev Respir Dis 1990; 142:523-528
2. Kollef MH. Ventilator-associated pneumonia. JAMA 1993; 270:1965-1970
3. Papazian L, Martin C, Meric B, et al. A reappraisal of blind bronchial sampling in the microbiologic diagnosis of nosocomial bronchopneumonia: a comparative study in ventilated patients. Chest 1993; 103:236-242
4. Kollef MH, Bock K, Richards R, et al. The safety and diagnostic accuracy of mini-bronchoalveolar lavage in patients with suspected ventilator-associated pneumonia. Ann Intern Med 1995; 122:743-748
5. Flanagan PG, Findlay PG, Magee JT, et al. The diagnosis of ventilator-associated pneumonia using nonbronchoscoping, non-directed lung lavages. Intensive Care Med 2000; 26:20-30
6. Fagon JY, Chastre J, Hance AJ, et al. Evaluation of clinical judgment in the identification and treatment of nosocomial in ventilated patients. Chest 1993; 103:547-553
7. Kollef MH, Ward S. The influence of mini-BAL cultures on patients' outcomes: implications for the antibiotic management of ventilator-associated pneumonia. Chest 1998; 113:412-420
8. Chastre J, Fagon JY, Soler P, et al. Quantification of BAL cells containing intracellular bacteria rapidly identifies ventilated patients with nosocomial pneumonia. Chest 1989; 95:190S-192S
9. Allaouchiche B, Jaumain H, Dumontet C, et al. Early diagnosis of ventilator-associated pneumonia: is it possible to define a cutoff value of infected cells in BAL fluid? Chest 1996; 110:1558-1565
10. Dotson RG, Pingleton SK. The effect of antibiotic therapy on recovery of intracellular bacteria from bronchoalveolar lavage in suspected ventilator-associated nosocomial pneumonia. Chest 1993; 103:541-546
11. Timsit JF, Misset B, Renaud B, et al. Effect of previous antimicrobial therapy on the accuracy of the main procedures used to diagnose nosocomial pneumonia in patients who are using ventilation. Chest 1995; 108:1036-1040
12. Le Gall JR, Lemeshow S, Saulnier F. A new Simplified Acute Physiology Score (SAPS II) based on a European/North American multicenter study. JAMA 1993; 270:2957-2963
13. De Brauwer E, Jacobs J, Nieman F, et al. Test characteristies of acridine orange, Gram's, and May-Grünwald-Giemsa stains for enumeration of intracellular organisms in bronchoalveolar lavage fluid. J Clin Microbiol 1999; 37:427-429
14. Rouby J, Rossignon M, Nicholas M, et al. A prospective study of protected bronchoalveolar lavage in the diagnosis of nosocomial pneumonia. Anesthesiology 1989; 71:679-685
15. Meduri G, Chastre J. The standardization of bronchoscopic techniques for ventilator-associated pneumonia. Chest 1992; 102:557S-564S
16. Papazian L, Thomas P, Garbe L, et al. Bronchoscopic or blind sampling techniques for the diagnosis of ventilator-associated pneumonia. Am J Respir Crit Care Med 1995; 152:1982-1991
17. Hanley JA, McNeil BA. The meaning and use of the area under a receiver operating (ROC) curve. Radiology 1982; 143:29-36
18. DeLong ER, DeLong DM, Clarke-Pearson DL. Comparing the areas under two or more correlated receiver operating characteristic curves: a nonparametric approach. Biometrics 1988; 44:837-845
19. Aubas S, Aubas P, Capdevila X, et al. Bronchoalveolar lavage for diagnosing bacterial pneumonia in mechanically ventilated patients. Am J Respir Crit Care Med 1994; 149:860-866
20. Solé-Violán J, Rodríguez de Castro F, Rey A, et al. Usefulness of microscopic examination of intracellular organisms in lavage fluid in ventilator-associated pneumonia. Chest 1994; 106:889-894
21. Pugin J, Auckenthaler R, Mili N, et al. Diagnosis of ventilator-associated pneumonia by baeteriologie analysis of bronchoscopic and nonbronchoscopic "blind" bronchoalveolar lavage fluid. Am Rev Respir Dis 1991; 143:1121-1129
22. Torres A, El-Ebiary M, Padro L, et al. Validation of different techniques for diagnosis of ventilator-associated pneumonia: comparison with immediate postmortem pulmonary biopsy. Am J Respir Crit Care Med 1994; 149:324-331
23. Kirtland SH, Corley DE, Winterbauer RH, et al. The diagnosis of ventilator-associated pneumonia: a comparison of histologic, microbiologic, and clinical criteria. Chest 1997; 112:445-457
24. Rouby J, De Lassale E, Poete P, et al. Nosocomial bronchopneumonia in the critically ill: histologic and bacteriologic aspects. Am Rev Respir Dis 1992; 146:1059-1066
25. Sirvent JM, Torres A, Vidaur L, et al. Tracheal colonization within 24 h of intubation in patients with head trauma: risk factor for developing early-onset ventilator-associated pneumonia. Intensive Care Med 2000; 26:1369-1372
26. Souweine B, Veber B, Bedos JP, et al. Diagnostic accuracy of protected specimen brush and bronchoalveolar lavage in nosocomial pneumonia: impact of previous antimicrobial treatments. Crit Care Med 1998; 26:236-244
27. Niederman MS. Bronchoscopy for ventilator-associated pneumonia: show me the money (outcome benefit)! Crit Care Med 1998; 26:198-199
28. Wunderink RG. Mortality and the diagnosis of ventilator-associated pneumonia: a new direction. Am J Respir Crit Care Med 1998; 157:349-350
29. Fagon JY, Chastre J, Wolff M, et al. Invasive and non-invasive strategies for management of suspected ventilator-associated pneumonia: a randomized trial. Ann Intern Med 2000; 132:621-630