A radiochemical pyruvate dehydrogenase assay: Activity in heart

Analytical Biochemistry

Volumn 313, Issue 1, 2003, Pages 179-182

  Sterk, Joseph P ^a   Stanley, William C ^a   Hoppel, Charles L ^a,c,d   Kerner, Janos ^b,c  

^a CASE WESTERN RESERVE UNIVERSITY SCHOOL OF MEDICINE   (United States)

^b CASE WESTERN RESERVE UNIVERSITY   (United States)

^c LOUIS STOKES CLEVELAND VA MEDICAL CENTER   (United States)

^d Geriatric Research   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BIOCHEMISTRY;

DEHYDROGENASE ASSAYS; PYRUVATES;

CHEMISTRY;

HEART ENZYME; PYRUVATE DEHYDROGENASE;

CHROMATOGRAPHY; DOG; ELUTION; ENZYME ACTIVITY; ENZYME SUBSTRATE; FROZEN SECTION; HEART MUSCLE BIOPSY; ISOTOPE LABELING; MOLECULAR WEIGHT; NOTE; PRIORITY JOURNAL; RADIOACTIVITY; RADIOENZYMATIC ASSAY; SWINE; TISSUE HOMOGENATE;

CANIS FAMILIARIS; SUS SCROFA;

EID: 0037330830     PISSN: 00032697     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0003-2697(02)00538-9     Document Type: Article

References (16)

1. Maas E., Bisswanger H. Localization of the alpha-oxoacid dehydrogenase multienzyme complexes within the mitochondrion. FEBS Lett. 277:1990;189-190.
2. Behal R.H., Buxton D.B., Robertson J.G., Olson M.S. Regulation of the pyruvate dehydrogenase multienzyme complex. Annu. Rev. Nutr. 13:1993;497-520.
3. Stanley W.C., Chandler M.P. Energy metabolism in the failing heart: potential for therapeutic interventions. Heart Failure Rev. 7:2002;115-130.
4. Cooper R.H., Randle P.J., Denton R.M. Regulation of heart muscle pyruvate dehydrogenase kinase. Biochem. J. 143:1974;625-641.
5. Wieland O., Siess E., Schulze-Wethmar F.H., Funcke H.G., Winton B. Active and inactive forms of pyruvate dehydrogenase in rat heart and kidney: effect of diabetes, fasting and refeeding on pyruvate dehydrogenase interconversion. Arch. Biochem. Biophys. 143:1971;593-601.
6. Hansford R.G., Cohen L. Relative importance of pyruvate dehydrogenase interconversion and feed-back inhibition in the effect of fatty acids on pyruvate oxidation by rat heart mitochondria. Arch. Biochem. Biophys. 191:1978;65-81.
7. Gohil K., Jones D.A. A sensitive spectrophotometric assay for pyruvate dehydrogenase and oxoglutarate dehydrogenase complexes. Biosci. Rep. 3:1983;1-9.
8. Linn T.C., Pelley J.W., Pettit F.H., Hucho F., Randall D.D., Reed L.J. Alpha-keto acid dehydrogenase complexes. XV. Purification and properties of the component enzymes of the pyruvate dehydrogenase complexes from bovine kidney and heart. Arch. Biochem. Biophys. 148:1972;327-342.
9. Taylor S.I., Mukherjee C., Jungas R.L. Studies on the mechanism of activation of adipose tissue pyruvate dehydrogenase by insulin. J. Biol. Chem. 248:1973;73-81.
10. Coore H.G., Denton R.M., Martin B.R., Randle P.J. Regulation of adipose tissue pyruvate dehydrogenase by insulin and other hormones. Biochem J. 125:1971;115-127.
11. Constantin-Teodosiu D., Cederblad G., Hultman E. A sensitive radioisotopic assay of pyruvate dehydrogenase complex in human muscle tissue. Anal. Biochem. 198:1991;347-351.
12. Kerner J., Hoppel C.L. Radiochemical malonyl-CoA decarboxylase assay: activity and subcellular distribution in heart and skeletal muscle. Anal. Biochem. 306:2002;283-289.
13. Hall J.L., Stanley W.C., Lopaschuk G.D., Wisneski J.A., Pizzurro R.D., Hamilton C.D., McCormack J.G. Impaired pyruvate oxidation but normal glucose uptake in diabetic pig heart during dobutamine-induced work. Am. J. Physiol. 271:1996;H2320-H2329.
14. Stanley W.C., Hernandez L.A., Spires D., Bringas J., Wallace S., McCormack J.G. Pyruvate dehydrogenase activity and malonyl-CoA levels in normal and ischemic swine myocardium: effects of dichloroacetate. J. Mol. Cell. Cardiol. 28:1996;905-914.
15. Richards J.G., Heigenhauser G.J., Wood C.M. Lipid oxidation fuels recovery from exhaustive exercise in white muscle of rainbow trout. Am. J. Physiol. 282:2002;R89-R99.
16. Schoeder H., Knight R.J., Kofoed K.F., Schelbert H.R., Buxton D.B. Regulation of pyruvate dehydrogenase activity and glucose metabolism in post-ischemic myocardium. Biochim. Biophys. Acta. 1406:1998;62-72.