Quantification of feto-maternal haemorrhage (FMH) by flow cytometry: Anti-fetal haemoglobin labelling potentially underestimates massive FMH in comparison to labelling with anti-D

Transfusion Medicine

Volumn 13, Issue 1, 2003, Pages 25-34

  Kennedy, Glen A ^a   Shaw, R ^a   Just, S ^a   Bryson, G ^a   Battistutta, F ^a   Rowell, J ^a   Williams, B ^a  

^a ROYAL BRISBANE AND WOMEN'S HOSPITAL   (Australia)

Author keywords

Anti D; Anti HbF; Feto maternal haemorrhage; Flow cytometry

Indexed keywords

HEMOGLOBIN F; HEMOGLOBIN F ANTIBODY; RHESUS D ANTIBODY; UNCLASSIFIED DRUG;

ANTIBODY LABELING; ARTICLE; DEVICE; DIAGNOSTIC ACCURACY; DIAGNOSTIC ERROR; DIAGNOSTIC TEST; DIAGNOSTIC VALUE; FETOMATERNAL TRANSFUSION; FETUS; FETUS CELL; FLOW CYTOMETRY; HUMAN; HUMAN CELL; HUMAN TISSUE; IMMUNOPROPHYLAXIS; MEDICAL AUDIT; QUANTITATIVE ANALYSIS; QUANTITATIVE ASSAY; QUANTITATIVE DIAGNOSIS; UMBILICAL CORD BLOOD;

EID: 0037329320     PISSN: 09587578     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1365-3148.2003.00416.x     Document Type: Article

References (21)

1. Al-Mufti, R., Hambley, H., Farzaneh, F. & Nicolaides, K.H. (2000) Fetal and embryonic hemoglobins in erythroblasts of chromosomally normal and abnormal fetuses at 10-40 weeks of gestation. Haematologica, 85, 690-693.
2. Bromilow, I.M. & Duguid, J.K.M. (1997) Measurement of feto-maternal haemorrhage: a comparative study of three Kleihauer techniques and two flow cytometry methods. Clinical and Laboratory Haematology, 19, 137-142.
3. Chapman, J.F., Bain, B.J., Bates, S.C., Knowles, S.M., Shwe, K.H., Parker-Williams, J., Robson, L. & Robson, S.C. (1999) The estimation of fetomaternal haemorrhage. Transfusion Medicine, 9, 87-92.
4. Dacie, J.V. & Lewis, S.M. (1984) Practical Haematology, 6th edn. Churchill Livingstone, New York.
5. Davis, B.H. (2001) Diagnostic advances in defining erythropoietic abnormalities and red blood cell diseases. Seminars in Hematology, 38, 148-159.
6. Davis, B.H., Olsen, S., Bigelow, N.C. & Chen, J.C. (1998) Detection of fetal red cells in fetomaternal hemorrhage using a fetal hemoglobin monoclonal antibody by flow cytometry. Transfusion, 38, 749-756.
7. Higgs, D.R., Thien, S.L. & Wood, W.G. (2001) Human haemoglobin. In: The Thalassaemia Syndromes (eds Weatherall, D. & Clegg, J.) 100-102. Blackwell Science Ltd, Oxford.
8. Huang, C.H., Liu, P.Z. & Cheng, J.G. (2000) Molecular biology and genetics of the Rh blood group system. Seminars in Hematology, 37, 150-165.
9. Janssen, W.C.M. & Hoffmann, J.J.M.L. (2002) Evaluation of flow cytometric enumeration of foetal erythrocytes in maternal blood. Clinical and Laboratory Haematology, 24, 89-92.
10. Kumpel, B.M. (2000) Analysis of factors affecting quantification of fetomaternal hemorrhage by flow cytometry. Transfusion, 40, 1376-1383.
11. Kumpel, B.M. (2001) Labeling of D+ RBCs for flow cytometric quantification of fetomaternal hemorrhage after the RBCs have been coated with anti-D. Transfusion, 41, 1059-1063.
12. Lee, D., Contreras, M., Robson, S.C., Rodeck, C.H. & Whittle, M.J. (1999) Recommendations for the use of anti-D immunoglobulin for Rh prophylaxis. Transfusion Medicine, 9, 93-97.
13. VI red cells in D-negative mixtures by flow cytometry: implications for feto-maternal haemorrhage quantification and D typing policies for newborns. British Journal of Haematology, 104, 621-625.
14. Lloyd-Evans, P., Kumpel, B.M., Bromelow, I., Austin, E. & Taylor, E. (1996) Use of a directly conjugated monoclonal anti-D (BRAD-3) for quantification of fetomaternal hemorrhage by flow cytometry. Transfusion, 36, 432-437.
15. Mollison, P.L. (1972) Quantitation of transplacental haemorrhage. British Medical Journal, 3, 31-34.
16. Mollison, P.L., Engelfriet, C.P. & Contreras, M. (1997) Blood Transfusion in Clinical Medicine. Blackwell Science Ltd, Oxford.
17. Nance, S.J., Nelson, J.M., Arndt, P.A., Lam, H.C. & Garratty, G. (1989) Quantitation of fetal-maternal hemorrhage by flow cytometry. American Journal of Clinical Pathology, 91, 288-292.
18. Nelson, M., Popp, H., Horky, K., Forsyth, C. & Gibson, J. (1994) Development of a flow cytometric test for the detection of D-positive fetal cells after fetomaternal hemorrhage and a survey of the prevalence in D-negative women. Immunohematology, 10, 55-59.
19. Nelson, M., Zarkos, K., Popp, H. & Gibson, J. (1998) A flow-cytometric equivalent of the Kleihauer test. Vox Sanguinis, 75, 234-241.
20. Polesky, H.F. & Sebring, E.S. (1981) Evaluation of methods for detection and quantification of fetal cells and their effect on Rh IgG usage. American Journal of Clinical Pathology, 76 (Suppl. 1), 525-529.
21. Urbaniak, S.J. (1998) The scientific basis of antenatal prophylaxis. British Journal of Obstetrics and Gynaecology, 105 (Suppl. 18), 11-18.