Hydroxyapatite chromatography: Altering the phosphate-dependent elution profile of protein as a function of pH

Analytical Biochemistry

Volumn 313, Issue 1, 2003, Pages 176-178

  Schröder, Ewald ^a   Jönsson, Thomas ^a   Poole, Leslie ^a  

^a WAKE FOREST SCHOOL OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BIOCHEMISTRY; CHEMISTRY;

ELUTION PROFILES; HYDROXYAPATITE CHROMATOGRAPHY;

HYDROXYAPATITE;

BUFFER; HYDROXYAPATITE; PHOSPHATE; PROTEIN;

ADSORPTION; COLUMN CHROMATOGRAPHY; CONCENTRATION (PARAMETERS); ELUTION; NOTE; PARTICLE SIZE; PH; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN BINDING; PROTEIN PURIFICATION; ROOM TEMPERATURE; TEMPERATURE;

EID: 0037328565     PISSN: 00032697     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0003-2697(02)00567-5     Document Type: Article

References (9)

1. Tiselius A., Hjerten S., Levin O. Protein chromatography on calcium phosphate columns. Arch. Biochem. Biophys. 65:1956;132-155.
2. Gorbunoff M.J. Protein chromatography on hydroxyapatite columns. Deutscher M.P. Methods Enzymology. vol. 182:1990;329-339 Academic Press, New York.
3. Schirch V., Hopkins S., Villar E., Angelaccio S. Serine hydroxymethyltransferase from Escherichia coli: purification and properties. J. Bacteriol. 163:1985;1-7.
4. Bernardi G. Chromatography of proteins on hydroxyapatite. Hirs C.H.W., Timasheff S.N. Methods Enzymology. vol. 27:1973;471-479 Academic Press, New York.
5. Rossano R., D'Elia A., Riccio P. One-step separation from lactose: recovery and purification of major cheese-whey proteins by hydroxyapatite: a flexible procedure suitable for small- and medium-scale preparations. Prot. Expr. Purif. 21:2001;165-169.
6. Scopes R.K. Protein Purification Principles and Practice. third ed. 1993;Springer, New York.
7. Ritz D., Lim J., Reynolds C.M., Poole L.B., Beckwith J. Conversion of a peroxiredoxin into a disulfide reductase by a triplet repeat expansion. Science. 294:2001;158-160.
8. Poole L.B., Ellis H.R. Flavin-dependent alkyl hydroperoxide reductase from Salmonella typhimurium. 1. Purification and enzymatic activities of overexpressed AhpF and AhpC proteins. Biochemistry. 35:1996;56-64.
9. Tonella L., Walsh B.J., Sanchez J-C., Ou K., Wilkins M.R., Tyler M., Frutiger S., Gooley A.A., Pescaru I., Appel R.D., Yan J.X., Bairoch A., Hoogland C., Morch F.S., Hughes G.J., Williams K.L., Hochstrasser D.F. '98 Escherichia coli SWISS-2DPAGE database update. Electrophoresis. 19:1998;1960-1971.