Versatile method employing basic techniques of genetic engineering to study the ability of low-molecular-weight compounds to bind covalently with DNA in cell-free systems

Analytical Biochemistry

Volumn 313, Issue 1, 2003, Pages 53-59

  Bartoszek, Agnieszka ^a   Sielenko, Anna ^a   Wȩsiora, Magdalena ^a  

^a GDANSK UNIVERSITY OF TECHNOLOGY   (Poland)

Author keywords

CC 1065; Cisplatin; Covalent binding to DNA; Restriction endonucleases

Indexed keywords

CELL ENGINEERING; DNA; ELECTROPHORESIS; FREE RADICALS; GENETIC ENGINEERING; MOLECULAR WEIGHT; OLIGONUCLEOTIDES;

CC-1065; CIS-PLATIN; COVALENT BINDING; COVALENT MODIFICATIONS; LOW MOLECULAR WEIGHT COMPOUNDS; MODIFIED OLIGONUCLEOTIDES; POLYACRYLAMIDE GEL ELECTROPHORESIS; RESTRICTION ENDONUCLEASES;

POLYMERASE CHAIN REACTION;

ADENINE; ANTINEOPLASTIC AGENT; CISPLATIN; DNA FRAGMENT; DOUBLE STRANDED DNA; ENDONUCLEASE; GUANINE; OLIGONUCLEOTIDE; RACHELMYCIN;

ARTICLE; BASE PAIRING; CELL FREE SYSTEM; COVALENT BOND; DNA ADDUCT; DNA BINDING; DNA CLEAVAGE; DNA MODIFICATION; DRUG DNA BINDING; GENE AMPLIFICATION; GENETIC ENGINEERING; MOLECULAR WEIGHT; POLYACRYLAMIDE GEL ELECTROPHORESIS; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; RESTRICTION SITE; TECHNIQUE;

EID: 0037328344     PISSN: 00032697     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0003-2697(02)00564-X     Document Type: Article

References (18)

1. Miller E.C., Miller J.A. Mechanisms of chemical carcinogenesis. Cancer. 47:1981;1055-1064.
2. Swenberg J.A., Richardson F.C., Boucheron J.A., Dyroff M.C. Relationships between DNA adduct formation and carcinogenesis. Environ. Health Perspect. 62:1985;177-183.
3. Melendez-Colon V.J., Luch A., Seidel A., Baird W.M. Cancer initiation by policyclic aromatic hydrocarbons results from formation of stable DNA adducts rather than apurinic sites. Carcinogenesis. 20:1999;1885-1891.
4. Iyer V.N., Szybalski W.A. A molecular mechanism of mitomycin C action: linking of complementary DNA strands. Proc. Natl. Acad. Sci. USA. 50:1963;355-362.
5. Hemminki K., Ludlum D.B. Covalent modification of DNA by antineoplastic agents. J. Natl. Cancer Inst. 73:1984;1021-1028.
6. Konopa J. Interstrand DNA crosslinking in tumor cells by 1-nitroacridines, anthracyclines and aminoanthraquinones. Pharmacol. Ther. 7(Suppl.):1990;83-94.
7. Harris C.C. Future directions in the use of DNA adducts as internal dosimeters for monitoring human exposure to environmental mutagens and carcinogens. Environ. Health Perspect. 62:1985;185-191.
8. 32P-Postlabeling analysis of non-radioactive aromatic carcinogen-DNA adducts. Carcinogenesis. 3:1982;1081-1092.
9. Phillips D.H., Castegnaro M. Standardization and validation of postlabelling methods: report of interlaboratory trials and production of recommended protocols. Mutagenesis. 14:1999;301-315.
10. Butour J.-L., Johnson N.P. Chemical reactivity of monofunctional platinum-DNA adducts. Biochemistry. 25:1986;4534-4539.
11. Reynolds V.L., Molineux I.J., Kaplan D.J., Swenson D.H., Hurley L.H. Reaction of the antitumor antibiotic CC-1065 with DNA. Location of the site of thermally induced strand breakage and analysis of DNA sequence specificity. Biochemistry. 24:1985;6228-6237.
12. Singer B. In vivo formation and persistance of modified nucleosides resulting from alkylating agents. Environ. Health Perspect. 62:1985;41-48.
13. Roberts J.J., Thompson A.J. The mechanism of action of antitumor platinum compounds. Prog. Nucleic Acid Res. Mol. Biol. 22:1979;71-109.
14. Hurley L.H., Reynolds V.L., Swenson D.H., Petzold G.L., Scahill T.A. Reaction of the antitumor antibiotic CC-1065 with DNA: structure of a DNA adduct with DNA sequence specificity. Science. 226:1984;843-844.
15. ′ -dimethylamino-n-propylamino)acridine, a new antitumor and DNA crosslinking agent, to macromolecules of subcellular fractions isolated from rat liver and HeLa cells Biochem. Pharmacol. 28:1979;3391-3402.
16. 32P-Postlabeling analysis of DNA adduct formation by antitumor drug nitracrine (Ledakrin) and other nitroacridines in different biological systems. Biochem. Pharmacol. 38:1989;1301-1312.
17. Cummings J., Bartoszek A., Smyth J.F. Determination of covalent binding to intact DNA, RNA, and oligonucleotides by intercalating anticancer drugs using high-performance liquid chromatography. Studies with doxorubicin and NADPH cytochrome P-450 reductase. Anal. Biochem. 194:1991;146-155.
18. 32P-Postlabeling analysis of adduct formed by mitoxantrone and ametantrone with DNA and homopolydeoxyribonucleotides after enzymatic activation. Chem.-Biol. Interact. 98:1995;1-14.