Determination of prostate specific antigen mRNA in peripheral blood by reverse transcriptase polymerase chain reaction and a simple chemiluminometric hybridization assay in a high-throughput format

Analytical Biochemistry

Volumn 313, Issue 1, 2003, Pages 97-105

  Emmanouilidou, Evaggelia ^a   Ioannou, Penelope C ^a   Christopoulos, Theodore K ^b,c   Polizois, Konstantinos ^d  

^a UNIVERSITY OF ATHENS   (Greece)

^b UNIVERSITY OF PATRAS   (Greece)

^c INSTITUTE OF CHEMICAL ENGINEERING SCIENCES   (Greece)

^d Gen District Hospital G Gennematas   (Greece)

Author keywords

[No Author keywords available]

Indexed keywords

ANTIGENS; BINDING SITES; BLOOD; DISEASES; LUMINESCENCE; MAMMALS; OLIGONUCLEOTIDES; PHOSPHATASES; PROBES; RNA; UROLOGY;

ALKALINE PHOSPHATASE; BENIGN PROSTATE HYPERPLASIA; BOVINE SERUM ALBUMINS; HYBRIDIZATION ASSAY; OLIGONUCLEOTIDE PROBES; PROSTATE SPECIFIC ANTIGEN; REVERSE TRANSCRIPTASE- POLYMERASE CHAIN REACTION; REVERSE-TRANSCRIPTASE POLYMERASE CHAIN REACTIONS;

POLYMERASE CHAIN REACTION;

ALKALINE PHOSPHATASE; BIOTIN; BOVINE SERUM ALBUMIN; CHEMICAL COMPOUND; DIOXETANE; MESSENGER RNA; OLIGONUCLEOTIDE; PRIMER DNA; PROSTATE SPECIFIC ANTIGEN; RECOMBINANT RNA; STREPTAVIDIN; UNCLASSIFIED DRUG;

ADSORPTION; AMINO ACID SEQUENCE; ANALYTIC METHOD; ARTICLE; BINDING SITE; BIOTINYLATION; BLOOD ANALYSIS; CANCER; CANCER CELL CULTURE; CANCER DIAGNOSIS; CELL COUNT; CHEMICAL LABELING; CHEMOLUMINESCENCE; CLINICAL ARTICLE; CLINICAL TRIAL; CONJUGATE; CONTROLLED CLINICAL TRIAL; CONTROLLED STUDY; DENATURATION; DIAGNOSTIC VALUE; HEAT; HUMAN; HYBRIDIZATION; IMMOBILIZATION; OLIGONUCLEOTIDE PROBE; PRIORITY JOURNAL; PROSTATE CANCER; PROSTATE HYPERTROPHY; PROTEIN BLOOD LEVEL; PROTEIN DETERMINATION; QUANTITATIVE ANALYSIS; REPRODUCIBILITY; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RNA EXTRACTION; STANDARD;

BOVINAE;

EID: 0037325769     PISSN: 00032697     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0003-2697(02)00566-3     Document Type: Article

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