Fluorescent chloramphenicol as a substitute for radioactive [14C]-chloramphenicol for CAT reporter assays in Plasmodium falciparum

Molecular and Biochemical Parasitology

Volumn 126, Issue 2, 2003, Pages 285-286

  Balu, Bharath ^a   Adams, John H ^a  

^a UNIVERSITY OF NOTRE DAME   (United States)

Author keywords

Chloramphenicol acetyl transferase; Plasmodium; Reporter assay; Transfection

Indexed keywords

CARBON 14; CHLORAMPHENICOL;

ACETYLATION; ANALYTIC METHOD; ARTICLE; CLONING; FLUORESCENCE; GENE CONTROL; GENE DISRUPTION; GENE EXPRESSION; GENETIC TRANSFECTION; MALARIA; NONHUMAN; PLASMODIUM FALCIPARUM; PRIORITY JOURNAL; RADIOACTIVITY;

ANIMALS; CARBON RADIOISOTOPES; CHLORAMPHENICOL; CHLORAMPHENICOL O-ACETYLTRANSFERASE; FLUORESCENT DYES; PLASMODIUM FALCIPARUM;

PLASMODIUM FALCIPARUM;

EID: 0037325019     PISSN: 01666851     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0166-6851(02)00285-2     Document Type: Article

References (4)

1. Murray I.A., Lewendon A., Williams J.A., Cullis P.M., Shaw W.V., Leslie A.G. Alternative binding modes for chloramphenicol and 1-substituted chloramphenicol analogues revealed by site-directed mutagenesis and X-ray crystallography of chloramphenicol acetyltransferase. Biochemistry. 30:1991;3763-3770.
2. Murray I.A., Lewendon A., Williams J.A., Cullis P.M., Lashford A.G., Shaw W.V. A novel substrate for assays of gene expression using chloramphenicol acetyltransferase. Nucleic Acids Res. 19:1991;6648.
3. Crabb B.S., Triglia T., Waterkeyn J.G., Cowman A.F. Stable transgene expression in Plasmodium falciparum. Mol. Biochem. Parasitol. 90:1997;131-144.
4. Wu Y., Kirkman L.A., Wellems T.E. Transformation of Plasmodium falciparum malaria parasites by homologous integration of plasmids that confer resistance to pyrimethamine. Proc. Natl. Acad. Sci. U.S.A. 93:1996;1130-1134.