Methyl green-pyronin Y staining of nucleic acids: Studies on the effects of staining time, dye composition and diffusion rates

Biotechnic and Histochemistry

Volumn 78, Issue 1, 2003, Pages 27-33

  Prentø, Poul ^a   Lyon, H O ^a  

^a UNIVERSITY OF COPENHAGEN   (Denmark)

Author keywords

Differential staining; Kinetics; Methyl green pyronin Y; Nucleic acid staining; Rate model

Indexed keywords

BRILLIANT GREEN; CELL DNA; HEPARIN; METHYL GREEN; NUCLEIC ACID; PYRONINE; RNA;

ANIMAL TISSUE; ARTICLE; BINDING KINETICS; COMPUTER PROGRAM; COMPUTER SYSTEM; CONCENTRATION (PARAMETERS); CONTROLLED STUDY; DIFFUSION COEFFICIENT; DNA CONTENT; DNA DETERMINATION; FILTER; HISTOCHEMISTRY; HYPOTHESIS; MEDICAL TECHNOLOGY; METHODOLOGY; NONHUMAN; PH MEASUREMENT; RAT; RNA ANALYSIS; TISSUE SECTION;

ANIMALS; DIFFUSION; DNA; HEPARIN; KINETICS; LIVER; METHYL GREEN; NECK; PANCREAS; PYRONINE; QUALITY CONTROL; RATS; RNA, FUNGAL; STAINING AND LABELING; SWINE;

ANIMALIA;

EID: 0037309423     PISSN: 10520295     EISSN: None     Source Type: Journal    
DOI: 10.1080/10520290312120006     Document Type: Article

References (25)

1. Andersen AP, Jakobsen P, Lyon H, Hoyer PE (1986) purification of methyl green using polyamide. Histochem. J. 18: 461-462.
2. Brachet J (1940) La detection histochimique des acides pentosenucleiques. C. R. Soc. Biol. 133: 88-90.
3. Goldstein DJ (1961) Mechanism of differential staining of nucleic acids. Nature (Lond.) 191: 407-408.
4. Goldstein DJ (1962) Differential staining of nucleic acids. Nature (Lond.) 193: 999-1001.
5. Goldstein DJ (1963) An approach to the thermodynamics of histological dyeing illustrated by experiments with Azur A. Q. J. Microsc. Sci. 104: 413-439.
6. Goldstein DJ, Horobin RW (1974) Rate factors in staining by alcian blue. Histochem. J. 6: 157-174.
7. Horobin RW (1974) A preliminary quantitative study of the "rate of staining" model for rationalising various mixed acid dye stains ("trichromes"). Proc. Roy. Microsc. Soc. 9: 110.
8. Horobin RW (1982) Histochemistry: an Explanatory Outline of Histochemistry and Biophysical Staining. Gustav Fischer Verlag, Stuttgart, New York. pp. 56-110.
9. Horobin RW, Bennion PJ (1973) The interrelation of the size and substantivity of dyes; the role of van der Waals attractions and hydrophobic bonding in biological staining. Histochemie 33: 191-204.
10. Horobin RW, Flemming, L (1980) Structure-staining relationships in histochemistry and biological staining. II. Mechanistic and practical aspects of the staining of elastic fibres. J. Microsc. 119: 357-372.
11. Horobin RW, Goldstein DJ (1972) Impurities and staining characteristics of alcian blue samples. Histochem. J. 4: 391-399.
12. Høyer PE, Lyon H, Jakobsen P, Andersen AP (1986) Standardized methyl green-pyronin Y procedures using pure dyes. Histochem. J. 18: 90-94.
13. Jakobsen P, Andersen AP, Lyon H, Treppendahl S (1983) Preparation and characterization of pyronin Y. Microsc. Acta 87: 41-47.
14. Jakobsen P, Andersen AP, Lyon H (1984) Preparation and characterization of methyl green tetrafluoroborate. Histochemistry 81: 177-179.
15. Kasten FH (1962) Comparisons of pyronin dyes obtained from various commercial sources. I. History. Stain Technol. 37: 265-275.
16. Kasten FH (1967) Cytochemical studies with acridine orange and the influence of dye contaminants on the staining of nucleic acids. Int. Rev. Cytol. 21: 141-202.
17. Lyon H, Holm I, Prentø P, Balslev E (1995) Non-hazardous organic solvents in the paraffin-embedding technique: a rational approach. Aliphatic monoesters for clearing and dewaxing: butyldecanoate. Histochemistry 103: 263-269.
18. Marshall PN, Horobin RW (1973) Measurements of the affinities of basic and "mordant" dyes for various tissue substrates. Histochemie. 36: 303-312.
19. Pappenheim A (1899) Vergleichende Untersuchungen über die elementare Zusammensetzung des rothen Knochenmarkes einiger Säugetiere. Virchows Archiv. Path. Anat. Physiol. 157: 19-76.
20. Prentø P (1993) Van Gieson's picrofuchsin. The staining mechanisms for collagen and cytoplasm, and an examination of the dye diffusion rate model of differential staining. Histochemistry 99: 163-174.
21. Prentø P (2001) A contribution to the theory of biological staining based on the principles for structural organization of biological macromolecules. Biotech. & Histochem. 76: 137-162.
22. Puchtler H, Meloan SN, Waldrop FS (1988) Are picrodye reactions for collagens quantitative? Histochemistry 88: 243-256.
23. Scott JE (1967) On the mechanism of the methyl green-pyronin stain for nucleic acids. Histochemie 9: 30-47.
24. Telford CB (1974) Nucleic acids and methyl green-pyronin: fixation, pyronin purification and theory of staining. Can. J. Med. Technol. 36: 10-24.
25. Unna PG (1902) Eine Modifikation der Pappenheimschen Färbung auf Granuloplasma. Monatsch. Prakt. Dermat. 35: 76-80.