Monoclonal antibodies to human cartilage oligomeric matrix protein: Epitope mapping and characterization of sandwich ELISA

Clinica Chimica Acta

Volumn 328, Issue 1-2, 2003, Pages 59-69

  Vilím, Vladimír ^a   Vobůrka, Zdeněk ^b   Vytášek, Richard ^a,c   Šenolt, Ladislav ^a   Tchetverikov, Ilja ^d,e   Kraus, Virginia B ^f   Pavelka, Karel ^a  

^a INSTITUTE OF RHEUMATOLOGY   (Czech Republic)

^b INSTITUTE OF ORGANIC CHEMISTRY AND BIOCHEMISTRY   (Czech Republic)

^c CHARLES UNIVERSITY   (Czech Republic)

^d LEIDEN UNIVERSITY MEDICAL CENTER   (Netherlands)

^e TNO   (Netherlands)

^f DUKE UNIVERSITY MEDICAL CENTER   (United States)

Author keywords

COMP; Epitope mapping; Monoclonal antibodies; Sandwich ELISA; Thrombospondin 5

Indexed keywords

CARTILAGE OLIGOMERIC MATRIX PROTEIN; EPIDERMAL GROWTH FACTOR; EPITOPE; MONOCLONAL ANTIBODY; TRYPSIN;

AMINO TERMINAL SEQUENCE; ARTICLE; BODY FLUID; CLINICAL ARTICLE; CONTROLLED STUDY; ENZYME LINKED IMMUNOSORBENT ASSAY; GENE MAPPING; HUMAN; PRIORITY JOURNAL; PROTEIN ANALYSIS; RHEUMATOID ARTHRITIS; SYNOVIAL FLUID LEVEL;

ANTIBODIES, MONOCLONAL; ENZYME-LINKED IMMUNOSORBENT ASSAY; EPITOPE MAPPING; EXTRACELLULAR MATRIX PROTEINS; GLYCOPROTEINS; HUMANS; SYNOVIAL FLUID;

EID: 0037306401     PISSN: 00098981     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0009-8981(02)00375-3     Document Type: Article

References (24)

1. Bornstein P., Sage E.H. Thrombospondins. Methods Enzymol. 245:1994;62-85.
2. Chen H., Deere M., Hecht J.T., Lawler J. Cartilage oligomeric matrix protein is a calcium-binding protein, and a mutation in its type 3 repeats causes conformational changes. J. Biol. Chem. 275:2000;26538-26544.
3. Oldberg A., Antonsson P., Lindblom K., Heinegård D. COMP (cartilage oligomeric matrix protein) is structurally related to the thrombospondins. J. Biol. Chem. 267:1992;22346-22350.
4. Rosenberg K., Olsson H., Morgelin M., Heinegård D. Cartilage oligomeric matrix protein shows high affinity zinc-dependent interaction with triple helical collagen. J. Biol. Chem. 273:1998;20397-20403.
5. Thur J., Rosenberg K., Nitsche D.P., Pihlajamaa T., Ala-Kokko L., Heinegård D.et al. Mutations in cartilage oligomeric matrix protein causing pseudoachondroplasis and multiple epiphyseal displasia affect binding of calcium and collagen I, II, and IX. J. Biol. Chem. 276:2001;6083-6092.
6. Holden P., Meadows R.S., Chapman K.I., Grant M.E., Kadler K.E., Briggs M.D. Cartilage oligomeric matrix protein interacts with type IX collagen, and disruptions to these interactions identify a pathogenetic mechanism in a bone dysplasia family. J. Biol. Chem. 276:2001;6046-6055.
7. Efimov V.P., Lustig A., Engel J. The thrombospondin-like chains of cartilage oligomeric matrix protein are assembled by a five-stranded α-helical bundle between residues 20 and 83. FEBS Lett. 341:1994;54-58.
8. Guo Y., Bozic D., Malashkevich V.N., Kammerer R.A., Schulthess T., Engel J. All-transretinol, vitamin D and other hydrophobic compounds bind to the axial pore of the five-stranded coiled-coil domain of cartilage oligomeric matrix protein. EMBO J. 17:1998;5265-5272.
9. Fang C., Carlson C.S., Leslie M.P., Tulli H., Stolerman E., Perris R.et al. Molecular cloning, sequencing, and tissue and developmental expression of mouse cartilage oligomeric matrix protein (COMP). J. Orthop. Res. 18:2000;593-603.
10. Newton G., Weremowics S., Morton C.C., Copeland N.G., Gilbert D.J., Jenkins N.A.et al. Characterization of human and mouse cartilage oligomeric matrix protein. Genomics. 24:1994;435-439.
11. Stevens J.W. Pseudoachondroplastic dysplasia: an Iowa review from human to mouse. Iowa Orthop. J. 19:1999;53-65.
12. Kumar S., Connor J.R., Dodds R.A., Halsey W., VanHorn M., Mao J.et al. Identification and initial characterization of 5000 expressed sequenced tags (ESTs) each from adult human normal and osteoarthritic cartilage cDNA libraries. Osteoarthr. Cartil. 9:2001;641-653.
13. Otterness I.G., Swindell A.C., Zimmerer R.O., Poole A.R., Ionescu M., Weiner E. An analysis of 14 molecular markers for monitoring osteoarthritis: segregation of the markers into clusters and distinguishing osteoarthritis at baseline. Osteoarthr. Cartil. 8:2000;180-185.
14. Sharif M., Saxne T., Shepstone L., Kirwan J.R., Elson C.J., Heinegård D.et al. Relationship between serum cartilage oligomeric matrix protein levels and disease progression in osteoarthritis of the knee joint. Br. J. Rheumatol. 34:1995;306-310.
15. Petersson I.F., Boegard T.B., Svensson D., Heinegård D., Saxne T. Changes in cartilage and bone metabolism identified by serum markers in early osteoarthritis of the knee joint. Br. J. Rheumatol. 37:1998;46-50.
16. Conrozier T., Saxne T., Shan Sei Fan C., Mathieu P., Tron A.-M., Heinegård D.et al. Serum concentrations of cartilage oligomeric matrix protein and bone sialoprotein in hip osteoarthritis: a one year prospective study. Ann. Rheum. Dis. 57:1998;527-532.
17. Clark A.G., Jordan J.M., Vilím V., Renner J.B., Dragomir A., Luta G.et al. Serum cartilage oligomeric matrix protein reflects osteoarthritis presence and severity: the Johnston county osteoarthritis project. Arthritis Rheum. 42:1999;2356-2364.
18. Vilím V., Olejárová M., Macháček S., Gatterová J., Kraus V.B., Pavelka K. Serum levels of cartilage oligomeric matrix protein (COMP) correlate with radiographic progression of knee osteoarthritis. Osteoarthr. Cartil. 10:2002;707-713.
19. Vilím V., Lenz M.E., Vytášek R., Masuda K., Pavelka K., Kuettner K.E.et al. Characterization of monoclonal antibodies recognizing different fragments of cartilage oligomeric matrix protein in human body fluids. Arch. Biochem. Biophys. 341:1997;8-16.
20. Hirano H., Komatsu S., Kajiwara H., Takagi Y., Tsunawa S. Microsequence analysis of the N-terminally blocked proteins immobilized on polyvinylidene difluoride membrane by Western blotting. Electrophoresis. 14:1993;839-846.
21. Vilím V., Vytášek R., Olejárová M., Macháček S., Gatterová J., Procházka B.et al. Serum cartilage oligomeric matrix protein reflects the presence of clinically diagnosed synovitis in patients with knee osteoarthritis. Osteoarthr. Cartil. 9:2001;612-618.
22. Rodbard D. Statistical quality control and routine data processing for radioimmunoassays and immunometric assays. Clin. Chem. 20:1974;1255-1270.
23. Zaia J., Boynton R.E., McIntosh A., Marshak D.R., Olsson H., Heinegård D.et al. Post-translational modification in cartilage oligomeric matrix protein. Characterization of the N-linked oligosaccharides by matrix-assisted laser desorption ionization time-of-flight mass spectrometry. J. Biol. Chem. 272:1997;14120-14126.
24. Saxne T., Heinegård D. Cartilage oligomeric matrix protein: a novel marker of cartilage turnover detectable in synovial fluid and blood. Br. J. Rheumatol. 31:1992;583-591.