Effects of blood collection methods on gelatin zymography of matrix metalloproteinases [5]

Clinical Chemistry

Volumn 49, Issue 2, 2003, Pages 339-340

  Mannello, Ferdinando ^a  

^a UNIVERSITY OF URBINO   (Italy)

Author keywords

[No Author keywords available]

Indexed keywords

EDETIC ACID; GELATIN; GELATINASE A; GELATINASE B; HEPARIN; MATRIX METALLOPROTEINASE; SILICA GEL; TUMOR MARKER;

ADULT; BLOOD SAMPLING; CENTRIFUGATION; CONTROLLED STUDY; DENSITOMETRY; DISEASE MARKER; ENZYME ACTIVITY; ENZYME ASSAY; ENZYME MECHANISM; HUMAN; LETTER; METHODOLOGY; NORMAL HUMAN; PROTEIN DEGRADATION; PROTEIN FAMILY; QUANTITATIVE ANALYSIS; THROMBOCYTE ACTIVATION; ZYMOGRAPHY;

BLOOD SPECIMEN COLLECTION; ENZYME PRECURSORS; GELATIN; HUMANS; MATRIX METALLOPROTEINASE 2; MATRIX METALLOPROTEINASE 9; MATRIX METALLOPROTEINASES;

EID: 0037305435     PISSN: 00099147     EISSN: None     Source Type: Journal    
DOI: 10.1373/49.2.339     Document Type: Letter

References (9)

1. Woessner JF, Nagase H. Matrix metalloproteinases and TIMPs. Oxford: Oxford University Press, 2000:223 pp.
2. McDonnell S, Morgan M, Lynch C. Role of matrix metalloproteinases in normal and disease processes. Biochem Soc Trans 1999;27:734-40.
3. Oberg A, Hoyhtya M, Tavelin B, Stenling R, Lindmark G. Limited value of preoperative serum analyses of matrix metalloproteinases (MMP-2, MMP-9) and tissue inhibitors of matrix metalloproteinases (TIMP-1, TIMP-2) in colorectal cancer. Anticancer Res 2000;20:1085-91.
4. Pucci-Minafra I, Minafra S, La Rocca G, Barranca M, Fontana S, Alaimo G, et al. Zymographic analysis of circulating and tissue forms of colon carcinoma gelatinase A (MMP-2) and B (MMP-9) separated by mono- and two-dimensional electrophoresis. Matrix Biol 2001;20:419-27.
5. Jung K, Lein M, Laube C, Lichtinghagen R. Blood specimen collection methods influence the concentration and the diagnostic validity of matrix metalloproteinases 9 in blood. Clin Chim Acta 2001;314:241-4.
6. Jung K, Laube C, Lein M, Lichtinghagen R, Tschesche H, Schnorr D, et al. Kind of sample as preanalytical determinant of matrix metalloproteinases 2 and 9 and tissue inhibitor of metalloproteinase 2 in blood [Technical Brief]. Clin Chem 1998;44:1060-2.
7. Makowski GS, Ramsby ML. Calibrating gelatin zymograms with human gelatinase standards. Anal Biochem 1996;236:353-6.
8. Mannello F. Circulating 92-kilodalton matrix metalloproteinase (MMP-9) activity is enhanced in the euglobulin plasma fraction of head and neck squamous cell carcinoma. Cancer 2002;95:in press.
9. Makowski GS, Ramsby ML. Interaction of amorphous calcium phosphate with fibrin in vitro causes decreased fibrinolysis and altered protease profiles: implications for atherosclerotic disease. Inflammation 2001;25:319-29.