Evaluation of drug-muscarinic receptor affinities by using cell membrane chromatography

Chinese Journal of Pharmacology and Toxicology

Volumn 17, Issue 1, 2003, Pages 70-73

  Hou, Jin ^b   Yuan, Bing Xiang ^a   He, Lang Chong ^a   Yang, Guang De ^a   Mi, Man ^b  

^a XI'AN JIAOTONG UNIVERSITY   (China)

^b Shanxi Medical College   (China)

Author keywords

Chromatography, cell membrane; Radioimmunoassay; Receptors, muscarinic

Indexed keywords

4 DIPHENYLACETOXY 1 METHYLPIPERIDINE; ACETYLCHOLINE; ATROPINE; BENZILIC ACID DERIVATIVE; LIGAND; MUSCARINIC RECEPTOR; NICOTINE; OTENZEPAD; PILOCARPINE; PIPERIDINE DERIVATIVE; PIRENZEPINE; RADIOLIGAND; SILICON DIOXIDE; TRILATE QUINUCLIDINYL BENZILATE; UNCLASSIFIED DRUG;

ACCURACY; ANIMAL TISSUE; ARTICLE; BINDING ASSAY; CELL MEMBRANE; CELL MEMBRANE CHROMATOGRAPHY; CHROMATOGRAPHY; CONTROLLED STUDY; CORRELATION ANALYSIS; DRUG RECEPTOR BINDING; HEART ATRIUM; IMMOBILIZATION; LIGAND BINDING; NONHUMAN; ONLINE ANALYSIS; RAT; SURFACE PROPERTY; VALIDATION PROCESS;

ANIMALIA;

EID: 0037301713     PISSN: 10003002     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (4)

1. He LC, Geng XD. Cell membrane chromatography-a new technique in studying drug-receptor interaction [A]. In: Zhou TH, Liu GQ, ed. Biomedical Chromatography Progress [M]. Beijing: Chemical Industry Publishing House, 1996. 8-10.
2. Michael ID, Robert PS. Ligand interactions with membrane-bound porcine atrial muscarinic receptors [J]. Biochemistry, 1980, 19(15):3407-3413.
3. Nedoma J, Slavikova J, Tucek S. Muscarinic acetylcholine receptors in the heart of rats before and after birth [J]. Pflugers Arch, 1986, 406(1):45-50.
4. He LC, Yang GD, Geng XD. Enzymatic activity and chromatographic characteristics of the cell membrane immobilized on silica surface [J]. Chin Sci Bull, 1999, 44(9):826-831.