The difference between sleep and anaesthesia is in the intracellular signal: Propofol and GABA use different subtypes of the GABAA receptor β subunit and vary in their interaction with actin

Acta Anaesthesiologica Scandinavica

Volumn 47, Issue 2, 2003, Pages 157-164

  Bjornstrom K ^a   Eintrei, C ^a  

^a LINKÖPING UNIVERSITY   (Sweden)

Author keywords

2, 6 di isopropylphenol; Anesthetic mechanisms; Cytoskeleton; GABAA receptor; Propofol; Tyrosine phosphorylation

Indexed keywords

4 AMINOBUTYRIC ACID; 4 AMINOBUTYRIC ACID A RECEPTOR; ACTIN; CALCIUM; PROPOFOL; RECEPTOR SUBUNIT; TYROSINE;

ANESTHESIA; ANIMAL CELL; ARTICLE; CELL MEMBRANE; CONTROLLED STUDY; CYTOSKELETON; CYTOSOL; DRUG PROTEIN BINDING; DRUG RECEPTOR BINDING; NERVE CELL CULTURE; NONHUMAN; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN LOCALIZATION; PROTEIN PHOSPHORYLATION; RAT; RECEPTOR BINDING; SLEEP;

ACTINS; ANESTHESIA; ANESTHETICS, INTRAVENOUS; ANIMALS; BLOTTING, WESTERN; CELL MEMBRANE; CELLS, CULTURED; CYTOSKELETON; CYTOSOL; GAMMA-AMINOBUTYRIC ACID; INDICATORS AND REAGENTS; NEURONS; PHOSPHORYLATION; PRECIPITIN TESTS; PROPOFOL; PROTEIN SUBUNITS; RATS; RECEPTORS, GABA-A; SIGNAL TRANSDUCTION; SLEEP; SUBCELLULAR FRACTIONS; TYROSINE;

EID: 0037300779     PISSN: 00015172     EISSN: None     Source Type: Journal    
DOI: 10.1034/j.1399-6576.2003.00007.x     Document Type: Article

References (34)

1. Cockshott ID. Propofol ('Diprivan') pharmacokinetics and metabolism: an overview. Postgrad Med J 1985: 61 (Suppl. 3): 45-50.
2. Concas A, Santoro G, Mascia MP, Serra M, Biggio G. The general anaesthetic propofol enhances the function of γ-aminobutyric acid-coupled chloride channel in the rat cerebral cortex. J Neurochem 1990: 55: 2135-2138.
3. 1 γ-aminobutyric acid type A receptors: actions of the anesthetics propofol and pentobarbital. Mol Pharmacol 1995: 47: 213-217.
4. A receptors: influence of receptor subunits. J Pharmacol Exp Ther 1995: 274: 353-360.
5. A receptor-ion channels: evaluation of pharmacological and physiological properties. Mol Pharmacol 1996: 49: 494-504.
6. Krasowski MD, Koltchine VV, Rick CE, Ye Q, Finn SE, Harrison NL. Propofol and other intravenous anesthetics have sites of action on the γ-aminobutyric acid type A receptor distinct from that for isoflurane. Mol Pharmacol 1998: 53: 530-538.
7. Hales TG, Lambert JJ. The actions of propofol on inhibitory amino acid receptors of bovine adrenomedullary chromaffin cells and rodent central neurones. Br J Pharmacol 1991: 104: 619-628.
8. A receptor complex. Brain Res 1992: 596: 238-242.
9. A receptors. Brain Res 1991: 542: 225-232.
10. A receptor-chloride ionophore complex in dissociated hippocampal pyramidal neurons of rat. Anesthesiology 1993: 79: 781-788.
11. Adodra S, Hales TG. Potentiation, activation and blockade of GABA (A) receptors of clonal murine hypothalamic GT1-7 neurones by propofol. Br J Pharmacol 1995: 115: 953-960.
12. 2 -subunits. Brain Res 1998: 784: 179-187.
13. A receptors in embryonic mouse neurons. Anesthesiology 1998: 89: 1444-1454.
14. A receptor subtypes. Int Rev Neurobiol 1995: 38: 95-138.
15. A receptors by tyrosine phosphorylation. Nature 1995: 377: 344-348.
16. A receptors. Mol Brain Res 1995: 31: 165-172.
17. Huang R-Q, Dillon GH. Maintenance of recombinant type A γ-aminobutyric acid receptor function: role of protein tyrosine phosphorylation and calcineurin. J Pharmacol Exp Ther 1998: 286: 243-255.
18. Bureau MH, Laschet JJ. Endogenous phosphorylation of distinct γ-aminobutyric acid type A receptor polypeptides by ser/thr and tyr kinase activities associated with the purified receptor. J Biol Chem 1995: 270: 26482-26487.
19. - ion channels modulated by multiple drug-binding sites. Trends Pharmacol Sci 1992: 13(12): 46-450.
20. A receptor and release of intracellular calcium. Acta Phys Scand 2002: 175: 227-235.
21. 3- subunits. J Biol Chem 1991: 266: 4478-4483.
22. A receptor subtypes differing in the β-subunit variant. J Biol Chem 1994: 269: 27100-27107.
23. A receptor. FEBS Lett 1991: 287 (1,2): 27-30.
24. Alberts B, Bray D, Lewis J, Raff M, Roberts K, Watson JD. Intracellular compartments and protein sorting. In: Molecular biology of the cell, 3rd edn. New York: Garland Publishing Inc, 1994: 551-599.
25. Thelen M, Rosen A, Nairn AC, Aderem A. Regulation by phopsphorylation of reversible association of a myristoylated protein kinase C substrate with the plasma membrane. Nature 1991: 351: 320-322.
26. Luna EJ, Hitt AL. Cytoskeleton-Plasma membrane interactions. Science 1992: 258: 955-964.
27. Jensen AG, Lindroth M, Sjölander A, Eintrei C. Propofol induces changes in the cytosolic free calcium concentrations and the cytoskeletal organization of cultured human glial cells and primary embryonic rat brain cells. Anesthesiology 1994: 81: 1220-1229.
28. Hansson E, Rönnbäck L. Primary cultures of astroglia and neurons from different brain regions. In: Shahar A, de Vellis J, Vernadakis A, Haber B, eds. A dissection and tissue culture manual of the nervous system. New York: Alan R. Liss, 1989: 92-104.
29. 2+. J Biol Chem 1990: 265: 20976-20981.
30. Laemmlie UK. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 1970: 227(259): 680-685.
31. Leader DP, Gall I, Campbell P, Frischauf AM. Isolation and characterization of cDNA clones from mouse skeletal muscle actin mRNA. DNA 1986: 5(3): 235-238.
32. Howard PK, Sefton BM, Firtel RA. Tyrosine phosphorylation of actin in Dictystelium associated with cell-shape changes. Nature 1993: 259: 241-244.
33. Thomas D, Patterson SD, Bradshaw RA. Src homolgous and collagen (Shc) protein binds to F-actin and translocates to the cytoskeleton upon nerve growth factor stimulation in PC12 cells. J Biol Chem 1995: 270: 28924-28931.
34. Oscarsson A, Massoumi R, Sjölander A, Eintrei C. Reorganization of actin in neurons after propofol exposure. Acta Anaesthesiol Scand 2001: 45: 1215-1220.